Objective To investigate the effect and molecular mechanism of botulinum neurotoxin serotype A (BoNT/A) heavy chain on neuron regeneration. Methods Cell culture, rats, immunofluorescence, SDS-PAGE and western blot, etc. were adopted in this study to explore the alterations of histone-3 acetylation (acetyl-H3 ) by local treatment of BoNT/A heavy chain to spinal cord injury (SCI) in rats (in vivo) or by adding it into cell culture (in vitro). Meanwhile, the relevance of acetyl-H3 to neurite out-growth based on SCI and cell culture with BoNT/A heavy chain application was approached as well. Results The application of BoNT/A heavy chain to cultured Neuro-2a cells increased the level of H3 acetylation. The increase of H3 acetylation was paralleled with the growth of neuritogenesis. Also, the neuronal treatment of BoNT/A heavy chain to SCI promoted the re-growth of neuronal processes surrounding the lesions. The growth of neuronal processes was positively correlated to the level of H3 acetylation. During the periods of BoNT/A heavy chain treatment in vivo or in vitro, the increase of H3 acetylation showed two peaks. Conclusions BoNT/A heavy chain increased the H3 acetylation, which might be one of its neuritogenic mechanisms.

随着近些年来我国经济取得的良好发展,使得人们的生活水平得到了稳步的提升,生活水平的提升促使人们对自身健康情况的关注度增加.当今社会的工作节奏飞快,工作压力巨大,这对于人们的健康有着一定程度的负面影响.而临床医学检测技术的出现,能够帮助人们对于自身的健康情况进行及时的了解,且能够为疾病的治疗提供准确的信息和更多的时间.可以说,临床医学检测技术已经成为了医学领域当前的主要研究内容.由于我国的人口数量较多,所以对于临床医疗检测技术的需求也相对较高,所以提升和发展我国的临床医学检测技术有着非常重要的意义.

目的:探讨人工重组A型肉毒毒素重链(BoNT/A重链)对大鼠脊髓损伤局部颈上神经节蛋白10(SCG10)表达、神经突起再生以及损伤侧后肢感觉及运动功能的影响,为阐明BoNT/A重链促神经突起生长的作用提供实验依据.方法:大鼠单侧腰段脊髓横断损伤模型基础上局部及鞘内给予BoNT/A重链;于用药后不同时间提取局部蛋白进行双向电泳,并根据双向电泳提示的蛋白点群变化的分子量和等电点,选取SCG10为目标蛋白行Western Blot检测及神经突起测定;通过抓力和热痛敏实验测试损伤侧后肢的运动和感觉功能情况.结果:①BoNT/A重链可干预脊髓损伤后局部蛋白谱的变化,MW位于18-25kDa、等电点在5-7范围的蛋白点可以作为蛋白点群变化的代表之一;②作为分子量位于18-25kDa/等电点在5-7的蛋白成分之一的SCG10在单纯损伤时较正常对照组其表达有所增高,给予BoNT/A重链后,SCG10的表达显著增强(P＜0.05);③免疫荧光结果显示:单纯损伤时SCG10的阳性表达主要分布于损伤周边细胞的胞体,应用BoNT/A重链后,SCG10阳性分布于胞浆和细胞突起且以损伤近头端的周边区域较为显著;④突起测定结果显示:BoNT/A重链可促进脊髓损伤周边神经细胞突起增长,包括突起数目增多及含有突起的神经细胞百分比增多(P＜0.05);⑤给予BoNT/A重链后,损伤侧后肢肌力明显优于单纯损伤组(P＜0.05),但感觉功能未见明显改善.结论:脊髓损伤局部给予BoNT/A重链可促进生长相关蛋白SCG10的表达并促进损伤周边神经细胞突起生长,改善肌肉抓力.BoNT/A重链有助于脊髓损伤后的再生修复.

Objective: To explore the surgical treatment experience of adult patent ductus arteriosus(PDA).Methods: Eighteen patients with adult patent ductus arteriosus(PDA) admitted from October 1998 to October 2009 were performed the surgical operation under deep hypothermia low flow(DHLF) cardiopulmonary bypass(CPB).Results: No surgical death happened in all patients.There were 5 patients with postoperative complications including 3 cases with low cardiac output syndrome(LCOS) and 2 cases with pericardial effusion but they were fully recovered by treatment.Conclusion: It is a safe and effective method to carry out the adult ductus arteriosus suture under deep hypothermia low flow cardiopulmonary bypass.