目的调查泸州市60岁以上老年人的营养状况并对其进行评价。方法在泸州市随机抽取两个社区内的682名60岁以上老年人进行身高体重等指标的测量,并计算体重指数(BMI)指数评价其营养状况。结果调查对象BMI均值为24.36,其中男性为24.23,女性为24.48,男女之间无差异;根据BMI指数评价的营养状况显示:超重和肥胖检出率分别是36.8%和13.6%,其中男性分别是37.9%和14.4%,女性分别是36.1%和13.1%。结论该地区居民超重和肥胖检出率较高,应加强该社区老年人群的健康教育和有针对性干预,以减少肥胖和相关并发症发生。
目的观察星形胶质细胞条件培养液(ACM)对鱼藤酮染毒嗜铬细胞瘤细胞(PC12)的神经保护作用。方法将新生SD大鼠中脑星形胶质细胞分离、纯化并培养至第三代后,收集星形胶质细胞条件培养液加入到鱼藤酮染毒PC12细胞中,观察其对染毒神经元形态、活力及其合成与释放一氧化氮(NO)、乳酸脱氢酶(LDH)和胞膜ATP酶(ATPase)的影响。结果20%ACM可明显改善染毒神经元的形态和活力;与对照组比较,20%ACM还有效减少了染毒神经元NO、LDH的合成和释放,保护和提高了ATPase活性。结论ACM明显促进鱼藤酮染毒PC12细胞的活力,其机制可能与减少NO、LDH合成释放及保护细胞膜ATPase的活性有关。
Objective To explore the toxic effect of rotenone on striatum and expression of connexin 43(CX43)in rats. Methods SD rats were administrated with rotenone via Alzet osmotic pump for 28 days;morphological changes of neurons and astrocytes in striatum were observed by light microscope with HE staining and immunohistochemistry method;while the expression of CX43 mRNA was detected by RT-PCR and the amount of CX43 protein was measured by Western blot. Results The results showed that the autonomic activities were decreased in rotenone exposed rats,quite similar to the manifestation of Parkinson's syndrome.The morphological changes of neurons and astrocytes in striatum were quite obvious,and the CX43 mRNA expression and CX43 protein amount were also increased significantly in rats treated with 2.0 mg/kg rotenone(P 0.05),however,which were obviously decreased in rats treated with 4.0 mg/kg rotenone(P 0.05). Conclusions It is suggested that rotenone may impair neurons in striatum of mesencephalon in rats and induce Parkinsonism-like symptoms,which might be attributed to the abnormal expression of CX43.
