Objective Comparison of two different disinfectant on the surface of a preterm infant incubator and the influence of the air colony count .Methods Under the same environment ,Incubator were randomly divided into experimental group and control group ,The experimental group:250mg/L iodine volts detergent wipe the surface of the casing ,Operation window ,shuikou storage;Control group:250mg/L "250"chlorine disinfectant ,With the experimental group .Compare two groups disin‐fectant effect .Results Two kinds of disinfectant effect comparison before and after disinfection( P <0 0.5);Two kinds of disinfectant after disinfection effect comparison( P <0 0.5) .Conclusion 250mg/L iodine v solution used for disinfection of w arm box ,sterilization effect is good .