Objective: To construct a recombinant CypA eukaryocyte expression vector,and to establish a high expression CypA hepatocellular carcinoma(HCC) cell model for further studying the function and mechanism of CypA in HCC.Methods: The full gene fragment of human CypA was cloned,and subcloned into the eukaryotic gene expression vector pcDNA3.1(+).Then the recombinant vector was transfected into the FHCC98 cells,a hepatocellular carcinoma cell line,which were cultured selectively with 800 μg/ml G4l8 to get a stable CypA expression cell line.The expression of CypA was examined by immunobloting and the proliferation rate was determined by MTT.Results: The eukaryotic expression vector pcDNA3.1-CypA was examined by two restrictive endonuclease digesting and sequence measuring,and both results were correct.The immunobloting results showed that the stable transfected pcDNA3.1-CypA-FHCC98 cell expressed higher level of CypA than the stable transfected pcDNA3.1-FHCC98 cell or untransfected FHCC98 cell.Comparing to control,pcDNA3.1-CypA-FHCC98 cell had a higher proliferation rate.Conclusion: The recombinant eukaryocyte expression vector and its stable expression HCC cell line were established.Over expression of CypA in FHCC98 cell could stimulate its proliferation.