软枣猕猴桃作为一种营养成分丰富、生物活性成分多样的药食同源性植物,是水果市场的畅销产品,同时在癌症治疗中具有良好功效,现已引起人们的广泛关注.为推动软枣猕猴桃产业的进一步发展,文章系统总结了北方软枣猕猴桃在药用价值、果实贮藏加工、品种选育及栽培管理方面的研究进展,以期为软枣猕猴桃的进一步开发利用提供参考.
A method of rapid isolation of total RNA was developed by using Irpex lacteus as test materials.Isolation time is short in the method: the whole course of isolating RNA from hypha only needs an hour.Purity of RNA is high: test it by ultraviolet spectrophotometer,A260/A280 is from 1.9 to 2.0.Integrity is high: the lighteness of 28S rRNA is as twice as the lighteness of 18S rRNA by analysis of gel electrophoresis;the method does not need use special apparatus and special medicines.The method has a few obvious advantages.Its purity and integrity could wholly make the need of molecular biology and other fields.
Thirty-four species of big wild fungi were collected and purified from natural forests throughout four nature reserves in northeast China,the oxidases of which were examined qualitatively.Six species of white rot fungi were selected to study of decolorization,a high efficient and wide range decolorization fungus Pycnoporus sanguineus was found in the decolorization of four kinds of dye.Orthogonal test indicated decolorization rate of gongo red reached 89.04% in nitrogen-limited and still light-prevent condition for five days.The decolorization rate reached 98.01% when added trace elements to culture medium.The dyes(Gongo red) decolorization rate obviously decreased in five days when the inducer was added to the culture medium.Data of orthogonal test was dealt with DPS software showed that the ultimate reason of dyes decolorization decrease was adding the guaiacol to culture medium.The dye decolorization tests were carried out with Pycnoporus sanguineus which was immobilized by sodium alginate and polyvinyl alcohol respectively.The result showed that: the former had a high decolorization rate of Congo red,the dye decolorization rate in 7 days was 92.86%,while the latter was only 22.97%.