Five aminoglycoside drug-resistance strains were selected from 89 clinical strains,to detect the expression of acrD gene by real-time RT-PCR,1 midrange drug-resistance strain,1 sensitive strain and ATCC25922 as control strains.The relative levels of acrD gene expression of drug-resistance strains was higher than the midrange drug-resistance strain,the sensitive strain and ATCC25922.The relative levels of acrD gene expression of five drug-resistance strains were 2.00,1.96,2.22,1.84 and 4.39,whereas it was 1.67 and 1.75 in susceptible strain and medium resistant strain,respectively.Remarkably,as the similar resistance level of strain NO.4 and NO.5,there is a big difference of acrD gene expression,the latter was 2.32 times of the former.The relative levels of acrD gene expression of the midrange drug-resistance strain was similar to the sensitive strain,both were higher than ATCC25922.The abover result proved that the expression of acrD gene are positive correlation with the level of aminoglycoside drug-resistant,while existing other mechanism contributes to aminoglycoside drug-resistant.

犬是杂食动物,极易发生呕吐.连续的呕吐会导致机体脱水和电解质流失(尤其是呕吐伴随腹泻).因此,做好犬呕吐疾病的诊疗具有重要意义.