李明亮 | AMiner学者主页

全面呈现 [李明亮] 的学术影响力

深度挖掘高质量成果与权威引用，让优秀看得见

研究兴趣

暂无数据

论文（3）

专利

基金项目

按年份排序按引用量排序主题筛选期刊级别筛选合作者筛选合作机构筛选

1MiR -22 -3p inhibits the migration and invasion of human hepatocellular carcinoma SMMC -7721 cells by regulating epithelial - mesenchymal transformation through Snail1

Hong-jie HE,Ming-liang LI,Guang-song XU

目的探讨miR-22-3p与Snail1的靶向关系,及其对人肝癌SMMC-7721细胞的迁移和侵袭的影响.方法用实时荧光定量聚合酶链反应法检测人正常肝细胞株LO2及4种肝癌细胞株中miR-22-3p及Snail1的相对表达水平并分析二者相关性.将细胞分为miR-22-3p对照组(miR-NC)、miR-22-3p模拟物组(miR-22-3p mimics)、Snail1空载体组(pLV-NC)、Snail1过表达组(pLV-Snail1)和miR-22-3 p mimics+pLV-Snail1组.用划痕实验和Transwell小室分别检测5组细胞的迁移和侵袭能力,用Western blot检测Snail1和上皮-间质转化相关蛋白的表达.结果在4种肝癌细胞株中,miR-22-3 p和Snail1表达呈显著负相关性(r=-0.966,P<0.05).miR-NC组、miR-22-3 p mimics组、pLV-NC组、pLV-Snail1组和miR-22-3 p mimics+pLV-Snail1组细胞划痕愈合率分别为(39.00±2.16)％,(11.67±2.05)％,(38.00±2.16)％,(72.33±4.11)％和(20.34±2.41)％,侵袭细胞数分别为(132.00±6.68),(47.00±6.16),(136.67±7.41),(205.33±6.60)和(73.67±4.50)个,Snail1蛋白相对表达量分别为(0.39±0.05),(0.15±0.03),(0.37±0.05),(0.88±0.06)和(0.24±0.02),上皮型钙黏蛋白相对表达量分别为(0.24±0.03),(0.84±0.06),(0.23±0.03),(0.18±0.02)和(0.34±0.04),神经型钙黏蛋白相对表达量分别为(0.52±0.03),(0.15±0.05),(0.51±0.03),(0.92±0.05)和(0.35±0.03),波形蛋白相对表达量分别为(0.45±0.05),(0.14±0.02),(0.43±0.04),(0.84±0.05)和(0.31±0.03).miR-22-3p mimics组与miR-NC组、pLV-Snail1组与pLV-NC组或miR-22-3p mimics组、pLV-Snail1组与miR-22-3p mimics+pLV-Snail1组的上述指标比较,差异均有统计学意义(P<0.05或P<0.01).结论 miR-22-3p能够通过靶向调控Snail1表达,进而抑制人肝癌SMMC-7721细胞的迁移和侵袭.

2019The Chinese Journal of Clinical Pharmacology（2019）引用：6

引用

AI阅读

2Both Intraoperative and Postoperative Choledochofiberscope Application in Cholelithiasis:327 Cases Clinical Research

LI Ming-liang,QIN Chun-hong,WU Jiang,HE Feng

Objective To investigate the application of choledochofiberscope in cholelithiasis.Methods Clinical data of 327 gallstone patients who had undergone choledochofiberscope treatment during and after surgical interventions were analyzed.Results 262 cases had undergone open surgery,the average postoperative stay was(11±2) days,the rate of residual stones was 14.12% down to 2.67% after postoperative choledochoscopy and remove of stones.Corresponding results was(4±1) days,4.61% down to 0,in 65 cases who had undergong laparoscopic with common bile duct exploration extraction of stones(LCBDE),none had been converted to open surgery.All cases avoided reoperation so far.Conclusions Choledochofiberscope treatment both during and after surgical interventions is uesful in diagnosis,surgical decision-making and the reduction the rate of residual stones.Conventional application of choledochofiberscope in cholelithiasis should be advocated.

2011Guide of China Medicine（2011）

引用

AI阅读

368例脾切除术后发热的临床探讨