Chun-Ming Zhang Shanghai Changzheng Hospital, Second Military Medical University Qi-Tong Chen Shanghai Changzheng Hospital, Second Military Medical University Cheng Wu Chinese PLA General Hospital Yuan-Qing Huang Bazhong Municipal Hospital of Traditional Chinese Medicine Bin Shi Shanghai Changzheng Hospital, Second Military Medical University Jian Shi (  shijian2008@smmu.edu.cn ) Shanghai Changzheng Hospital, Second Military Medical University

ObjectiveTo discover new proteomic biomarkers of hepatocellular carcinoma.MethodsSurface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry was used to discover biomarkers for differentiating hepatocellular carcinoma and chronic liver disease. A population of 50 patients with hepatocellular carcinoma and 33 patients with chronic liver disease was studied.ResultsTwelve proteomic biomarkers of hepatocellular carcinoma were detected in this study. Three proteomic biomarkers were highly expressed in hepatocellular carcinoma and nine proteomic biomarkers were highly expressed in chronic liver disease. The most valuable proteomic biomarker with m/z=11498 had no similar diagnostic value as α-fetoprotein.ConclusionSome of the twelve proteomic biomarkers may become new biomarkers of hepatocellular carcinoma.