目的 验证基于实时荧光PCR技术对中东呼吸综合征冠状病毒临床诊断检测方法的准确性和有效性.方法 选取MERS-CoV的E基因上游区域(up-E)和N2基因保守区作为扩增靶区域,设计特异性引物探针,通过实时荧光PCR扩增体系对中东呼吸综合征冠状病毒核糖核酸的up-E和N2基因进行定性检测,对203例模拟阳性样本、175例阴性和干扰样本进行检测验证.结果 203例模拟阳性样本和175例阴性和干扰样本检测结果与对照方法完全一致,符合率为100％.结论 初步验证MERS-CoV核酸检测方法可靠、准确.

目的:研制一种传染病快检集成背囊,用于战现场早期群发疫情病原体快速检测.方法:传染病快检集成背囊由箱体、伸缩拉杆、滚轮及支架组成,其中箱体由上箱体和下箱体组成.上箱体为常温仓,内部通过隔断设置了多个普通试剂存放室;下箱体为低温仓,设有多个特殊试剂存放室,内含降温装置.结果:该背囊与外部环境相对隔离,可有效保障内存试剂的性能,实现对12种传染病病原体的快速检测,可有效解决传染病防控现场快检操作步骤烦琐、携带不便、携带试剂存放条件有限等问题.结论:该背囊具有结构精巧合理、携带使用方便、试剂存放安全、功能集成一体化等特点,为传染病防控现场的快速筛检提供了可靠、有效的检验设备,值得推广使用.

目的 分析原发性胆汁性肝硬化(PBC)实验室指标,为临床诊治提供依据.方法 选取2016年解放军第三〇二医院诊断为PBC的患者593例,对其自身抗体指标抗线粒体抗体(AMA)、抗核抗体(ANA)、抗平滑肌抗体(SMA)、抗线粒体抗体-M2(AMA-M2)、抗可溶性肝抗原/肝胰抗原抗体(SLP)、抗核孔复合物糖蛋白210抗体(gp210)、核点型靶抗原蛋白(Sp100)、早幼粒白血病蛋白(PML)以及生化免疫学指标谷氨酰转肽酶(GGT)、碱性磷酸酶(ALP)、免疫球蛋白 A (IgA)、免疫球蛋白 M(IgM)、免疫球蛋白 G(IgG)进行检测并分析结果.结果 PBC患者血清内 ANA、AMA、SMA、AMA-M2、SLP/LP、gp210、Sp100的检出率显著高于慢性乙型肝炎组,差异均有统计学意义(P<0.05).ANA的主要核型是核膜型(225例,52.33%)以及核点型(90例,20.93%),滴度以中高滴度为主(≥1:320),复合核型也有相当比例(113例,26.28%).AM A以高滴度(≥1:1000)为主(470例,84.79%).AM A-M2的滴度以高滴度(≥"3+")为主(358例, 88.18%).PBC组患者的GGT、ALP、IgM、IgG与慢性乙型肝炎组患者比较均显著升高,差异有统计学意义(P<0.05), IgA差异无统计学意义(P>0.05).结论 各型自身抗体对于 PBC具有诊断意义,ALP、GGT、IgM 不明原因升高也是PBC特殊指征,临床应该重视各种实验室指标,对于PBC患者早诊断早治疗,积极改善PBC患者的生存质量.

Hepatocellular carcinoma (HCC) is a highly lethal malignant tumor evolved from cirrhosis. It is quite significant to seek accurate, easy markers for early warning and diagnosis of HCC. Through prospective cohort follow-up study and mass spectrometry, we discovered and verified a serum marker valuable for early warning and diagnosis. Follow-up observation was performed on cirrhosis patients. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was adopted to detect the serums of patients, and the serum polypeptides with a potential value in early HCC warning and diagnosis were screened. Electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) was exploited to identify these screened polypeptides. Moreover, the serum marker concentration was determined by ELISA to validate the clinical value of the serum marker. Among 109 cirrhosis patients followed up for two years, 29 patients (26.6%) finally progressed into HCC. MALDI-TOF MS shows that the concentration of a 3155.66Da polypeptide was significantly different between the patients that progressed into HCC and those not. Through MS/MS identification, it is confirmed that the polypeptide is inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4). The serum ITIH4 concentrations in two groups were measured with ELISA and compared with Alpha-fetoprotein (AFP). Results show that serum ITIH4 and AFP concentrations were negatively correlated (r=−0.263, p=0.0006), and the ITIH4 concentration had a significant intergroup difference (p=0.000). Receiver operating characteristic (ROC) curve indicates that its predictive value (area under the curve, AUC) is 0.667, superior to AFP. For the patients progressing into HCC, serum samples were separately collected when they were recruited and diagnosed as cirrhosis. Measurement on these samples reveals that ITIH4 was declining during the progression of HCC (p=0.006). By virtue of mass spectrometry, we discovered and identified a biomarker valuable for early HCC warning and diagnosis. This marker overperforms the commonly used AFP, demonstrating a bright prospect.

目的 评估肝硬化患者腹水直接检测降钙素原(PCT)和白介素6(IL-6)用来诊断自发性细菌性腹膜炎(SBP)的价值.方法 按照SBP的诊断标准,2015年1月至4月在解放军第三○二医院连续收集74例SBP肝硬化患者的腹水(SBP组)和75例无感染的肝硬化患者的腹水(对照组),检测患者的总胆红素、AST、ALT及凝血酶原活动度,电化学发光法检测患者腹水的PCT和IL-6,同时检测SBP组患者的血清PCT.SPSS13.0进行统计学分析.结果 肝硬化腹水患者男性明显多于女性,SBP组和对照组在性别、年龄比较差异无统计学意义(P＞0.05);SBP组的腹水分叶核细胞计数显著高于对照组(2634.3±7198对32.1±45.6,P=0.003),SBP组的AST显著高于对照组(276.5±868.7对66.1±51.9,P=0.044);SBP组腹水PCT和IL-6水平分别显著高于对照组(0.94±1.24 ng/mL对0.21±0.27 ng/mL,P＜0.001;3386.7±1551.9 pg/mL对1775.4±1551.9 pg/mL,P＜0.001).SBP组血液和腹水PCT具有相关性(R=0.771,95％CI:0.234～0.346);腹水PCT受试者工作曲线(ROC)的曲线下面积为0.783,95％CI:0.707～0.860,P＜0.001,PCT折点为0.4 ng/mL,敏感度为0.608,特异性为0.907.结论 肝硬化患者腹水直接检测PCT可以诊断肝硬化患者SBP,但敏感度不高.IL-6不太适用于肝硬化患者SBP的诊断.

Objective To evaluate a new reagent called reverse dot blot assay kit for hepatitis B virus geno-types(immunofluorescence method).Methods Three hundred and eighty-three serum samples were collect-ed.Another 50 cases of corresponding plasma were needed.Hepatitis B virus genotype A to D was detected by the diagnostic kit for hepatitis B virus genotypes(fluorescence immunochromatography assay).The gene se-quencing technique was regarded as a control method for genotype A,and other genotypes were determined by real-time quantitive PCR.The same sample was detected by using the experimental reagent and control reagent at the same time.If the results were inconsistent,another two previous detections were needed as the final re-sults.If the results of one more time detecting were still inconsistent,then the samples would be sequenced and the results were analyzed by kappa-test for symmetric measures.In addition,the results of the correspond-ing serum and plasma samples were analyzed to check the consistency.Results Fifteen genotype A samples were detected out by diagnostic kit,and 12 samples were detected out by the control reagent.For genotype B, C,D,the positive coincidence rates were of 98.00%,98.78%,87.50%,respectively;the negative coincidence rates were 99.06%,86.99%,100.00%,respectively;the total coincidence rates were 94.84%,94.84%, 99.73%,respectively;the value of kappa were 0.881,0.881,0.932,respectively.And the detecting results of plasm samples were same as that of the corresponding serum samples.Conclusion The experimental reagent was able to meet the requirements to the clinical diagnosis.

Dengue fever is caused by the dengue virus (DENV), and DENV1 is the prevalent epidemic serotype in south China. A new lateral flow assay (LFA) based on a near-infrared (NIR) fluorescent dye was developed to detect anti-DENV1 IgG antibodies. DyLight-800 was used as the marker conjugated to goat anti-human IgG antibodies, and recombinant dengue type 1 envelope protein was used as the capture protein on the test line. Twenty samples from patients infected with DENV1 and 160 negative controls were analyzed using this new NIR-LFA. The results of the NIR-LFA were compared with the results of Panbio Dengue IgG ELISA and the Dengue Duo IgM/IgG Cassette. Nineteen confirmed DENV1-positive samples were identified by NIR-LFA, giving 95% (19/20) sensitivity. No significant differences existed in the results when the 20 primary clinical samples were analyzed using NIR-LFA, Panbio ELISA, and the Dengue Duo Cassette. However, NIR-LFA had a lower limit of detection than IgG ELISA and Duo IgM/IgG Cassette did when analyzing a 2-fold dilution series of the 19 samples positively identified by NIR-LFA. When incorporated with an NIR POCT device, the new NIR-LFA was rapid, easy to use, and highly sensitive in detecting DENV1, and has potential for application to clinical diagnosis.

目的 调查隐匿性HBV感染(OBI)的情况.方法 连续收集2016年8月至2017年3月HBsAg(-)且其他HBV标志物阳性患者血清样本1570份,同时排除单独抗-HBs阳性患者.采用全自动磁珠法核酸提取及实时荧光定量PCR系统进行HBV DNA定量检测,同时采用罗氏CAP/Prep HBV检测系统复核.结果 1570份标本中共有71份HBV DNA阳性标本.1570份标本中,肝炎506份,肝硬化399份,肝占位41份,肝癌189份,其他诊断435份.HBV DNA阳性标本中,肝炎31份,肝硬化7份,肝占位3份,肝癌16份,其他14份.71份OBI标本中有7份为健康查体者标本.结论 OBI在人群中有一定的发生率,肝占位和肝癌患者OBI发生率较高,健康体检人群中仍可筛查出OBI者.

The early prediction values of diagnostic markers for hepatocellular carcinoma (HCC) are still unclear at present. This study evaluated the prediction value of ten serum markers in HCC. A total of 109 cases of hepatic cirrhosis patients were followed up for 36 months and the relationship between the lifetime risk of developing HCC and levels of serum markers was analyzed. 31.2 (34/109) percent of hepatic cirrhosis patients developed HCC during the study’s timeframe. Higher alpha-fetoprotein (AFP), alpha-fetoprotein-L3 (AFP-L3), alanine aminotransferase (ALT), and AFP-L3/AFP ratio levels are potential risk factors for malignization in hepatic cirrhosis patients (RR=2.99, 2.92, 2.72, and 2.34); serum Golgi protein 73 (GP73) level of hepatic cirrhosis patients decreased significantly after developing HCC (t=2.212;p=0.041). The detection of ALT, AFP, AFP-L3, and GP73 has a certain guiding significance to predict the risk of HCC in hepatic cirrhosis patients.

Objective To investigate the clinical features of patients with pure red cell aplasia (PRCA)associated with human parvovirus B19 (HPV B19)infection after liver transplantation.Methods The clinical data of 420 patients who underwent liver transplantation in 302 Hospital of PLA from July 2007 to July 2016 and were followed up regularly were collected,and among these patients,five had a progressive reduction in hemoglobin (Hb)level within a short period of time.Bone marrow cytological examination showed erythropoiesis disorders and positive HPV B19 IgM,and the patients were diagnosed with PRCA after the exclusion of other causes.The patients were given human gam-ma -globulin,glucocorticoids,and adjustment of immunosuppressants.The patients′clinical manifestations during treatment and the chan-ges in reticulocyte count (RC),Hb,white blood cell count (WBC),platelet count (PLT),and myelogram findings determined by periph-eral blood cell analysis were observed,as well as the changes in liver and renal function parameters.Results After the multimodality thera-py using human gamma -globulin,glucocorticoids,and red blood cell infusion,the five patients had significant alleviation in the symptoms of weakness,short breath,and dizziness,and the peripheral blood cell analysis showed recovery of Hb and RC,suggesting that anemia was corrected.Conclusion As for patients with PRCA associated with HPV B19 infection,early diagnosis and treatment with human gamma -globulin and glucocorticoids can achieve a good therapeutic effect.

More than 70% of hepatocellular carcinoma (HCC) cases develop as a consequence of liver cirrhosis (LC). Here we have evaluated the diagnostic potential of four serum biomarkers, and developed models for HCC diagnosis and differentiation from LC patients. Serum levels of α-fetoprotein (AFP), AFP-L3, des-γ-carboxy prothrombin (DCP), and Golgi protein 73 (GP73) were analyzed in 114 advanced HCC patients, 81 early stage HCC patients, and 152 LC patients. Multilayer perceptron (MLP) and radial basis function (RBF) neural networks were used to construct the diagnostic models. Using all stages, HCC diagnostic models had a higher sensitivity (>70%) than the individual serum biomarkers, whereas only early stage HCC diagnostic models had a higher specificity (>80%). The early stage HCC diagnostic models could not be used as HCC screening tools due to their low sensitivity (about 40%). These results suggest that a combination of the two models might be used as a screening tool to distinguish early stage HCC patients from LC patients, thus improving prevention and treatment of HCC.

Background: Differentiation of malignant from benign liver tumors remains a challenging problem. In recent years, mass spectrometry (MS) technique has emerged as a promising strategy to diagnose a wide range of malignant tumors. The purpose of this study was to establish classification models to distinguish benign and malignant liver tumors and identify the liver cancer-specific peptides by mass spectrometry.Material/ Methods: In our study, serum samples from 43 patients with malignant liver tumors and 52 patients with benign liver tumors were treated with weak cation-exchange chromatography Magnetic Beads (MB-WCX) kits and analyzed by the Matrix-Assisted Laser Desorption Time of Flight Mass Spectrometry (MALDI-TOF-MS). Then we established genetic algorithm (GA), supervised neural networks (SNN), and quick classifier (QC) models to distinguish malignant from benign liver tumors. To confirm the clinical applicability of the established models, the blinded validation test was performed in 50 clinical serum samples. Discriminatory peaks associated with malignant liver tumors were subsequently identified by a qTOF Synapt G2-S system.Results: A total of 27 discriminant peaks (p<0.05) in mass spectra of serum samples were found by ClinPro Tools software. Recognition capabilities of the established models were 100% (GA), 89.38% (SNN), and 80.84% (QC); cross-validation rates were 81.67% (GA), 81.11% (SNN), and 86.11% (QC). The accuracy rates of the blinded validation test were 78% (GA), 84% (SNN), and 84% (QC). From the 27 discriminatory peptide peaks analyzed, 3 peaks of m/z 2860.34, 2881.54, and 3155.67 were identified as a fragment of fibrinogen alpha chain, fibrinogen beta chain, and inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), respectively.Conclusions: Our results demonstrated that MS technique can be helpful in differentiation of benign and malignant liver tumors. Fibrinogen and ITIH4 might be used as biomarkers for the diagnosis of malignant liver tumors.

目的 探究HIV初筛试验与确证试验的结果之间的关系,通过随访研究确证试验不确定结果的抗体进展情况.方法 2014年1月-2016年8月解放军302医院HIV抗体初筛阳性的地方患者331例,初筛方法为化学发光法,以免疫印迹法(Westem blotting,WB)作为确证试验,分析结果.并对不确定患者进行随访,分析其出现的条带,对转阳以及转阴的结果汇总统计.结果 初筛S/CO值在1～5之间的有84例,阳性符合率为0.00％,5～20之间的有36例,阻性符合率为22.22％,20～100之间的有65例,阳性符合率为67.69％,大于100的有146例,阳性符合率为98.63％.免疫印迹法检测后,72例不确定结果中存在多种条带,终判定为阳性的病人有13例,判定为阴性的病人为38例,失访21例.结论 化学发光试剂存在有一定的假阳性,随着S/CO值的升高,其与确证试验的阳性符合率也会升高,但是高S/CO值的标本并不一定代表感染了HIV.同时免疫印迹法检测HIV也有一定的局限性,所以建议HIV抗体的报告应该用初筛试验、复检试验、确证试验的结果进行综合判断.

目的：分析肝硬化合并淋巴瘤患者的临床和实验室特点，提高对该病的认识。方法回顾调查患者的临床基本情况、原发病、实验室结果、治疗和预后；全自动生化分析仪检测患者肝功能结果，全血细胞分析仪检测血常规；骨髓穿刺进行骨髓检查分析。结果6例患者均为肝硬化，3例可以触及淋巴结肿大，4例深部淋巴结肿大；其做骨髓穿刺后，2例原始幼稚淋巴细胞，4例淋巴细胞异形性变；根据骨髓镜检高度怀疑其为淋巴瘤，淋巴穿刺活检证明为淋巴瘤。结论骨髓象检测可以对肝硬化患者合并淋巴瘤早期诊断提供重要线索。

Objective To identity microRNA (miRNA) diagnostic markers for chronic hepatitis B (CHB) and analyze the potential correlation between miRNA expression level and hepatitis B virus (HBV) replication.Methods We analyzed the expression level of 10 miRNAs in plasma samples from 50 CHB patients and 50 healthy Donors by Fluorescence quantitative PCR.A miRNA expression panel for chronic hepatitis B was found out through analysis of expression differences,accordingly a regression diagnosis model was established.The relationship between the miRNAs expression level and HBV DNA viral load was also visualized by Spearman rank correlation analysis.Results The expression level of 8 miRNAs were significantly different between CHB patients and healthy controls.miR-451 a was down-regulated and other 7 miRNAs were up-regulated.Logistic model based on data from miR-122-5p,miR-27a-3p and miR-451a showed that area under curve (AUC) was 1,and sensitivity and specificity of the diagnostics CHB from healthy sample reached 100％ in these case.The expression level of miR-122-5p had a significantly positive correlation with HBV DNA load.Conclusions For diagnosis of CHB,miR-122-5p,miR-27a-3p and miR451a could be used as new markers which play an important role in occurrence and development of CHB.

Objective: To investigate the application value of urinary sediment workstation with the index of LEU, BAC, MUC in diagnosis of urinary tract infection. Methods: The levels of LEU, BAC and MUC from urine of 292 patients with hepatitis were detected by Lab UMat urinary sediment workstation. The serum levels of PCT, CRP, DBIL, TBIL and ALT were detected by electrochemical luminescence methods and Immunoturbidimetric assays individually. Taking bacterial culture results as gold standard, the changing characterized and diagnostic value of LEU, BAC and MUC were evaluated. Results: Gram-negative bacteria of urine infection were the most common bacteria which accounted for 80.5%, while Escherichia coli took the most. And for Gram-positive bacteria Enterococcus faecium was the most common bacteria. Based on the gold standard of the bacteria cultures, the area under the receiver operating curve(AUC) of BAC was 0.82, and the sensitivity, specificity were 77.83% and 90.2% individually. The AUC of LEU was 0.85, and the sensitivity, specificity were 87.6% and 83.3% individually. The AUC of MUC was 0.63, and the sensitivity, specificity were 60.3% and 72.7% individually. With the rising levels of urinary BAC, the levels of LEU, MUC and PCT, DBIL, TBIL were significantly elevated(t=13.8, t=12.3, t=4.5, t=15.2, t=11.6; and t=16.7, t=17.1, t=10.8, t=20.3, t=19.6, P0.01). Conclusion: Lab UMat Urinary Sediment Workstation had a high value in diagnosis of urinary tract infection.

Objective To compare the coincidence rate and the equivalence of quantitative detection between the procalcitonin(PCT ) quantitative detection kit(immunochromatography) researched and developed by the Maans‐han Guosheng Biotechnology Co .,Ltd .and the approved marketed similar products to judge whether the developed product meeting the clinical use requirements .Methods 122 cases of various clinical samples collected from March to April 2014 were selected and detected by the two kinds of method .The detection results by using the two kinds of re‐agent kit were compared .The statistical software adopted the Bland‐Altman analysis ,regression analysis ,intraclass correlation analysis and Pearson product correlation analysis .The consistency of the two kinds of detection method was analyzed .Results When the cut off value was 0 .5 ng/mL ,the analysis consistency of the two kinds of method was 99 .18% ;when the cut off value was 2 ng/mL ,which was 95 .08% .The consistency limit values were 0 .205 7 and 0 .088 4 ,which were less than the profession value ± 0 .5 .The Pearson correlation coefficient was 0 .979 5 ,the Spearman correlation coefficient was 0 .972 1 ,showing the detection results by the two kinds of reagent kit had a sig‐nificant correlation(P< 0 .01) .Conclusion The two kinds of reagent kit possess the equivalence .

Objective The aim of this study is to analyze the clinical features of patients with Ebola virus (EBOV) infection at admission and to provide information for clinicians to manage patients with suspected Ebola virus disease (EVD).Methods Sixty-two suspected EVD cases visited China-Sierra leone Friendship Hospital which was set up by the first medical team from the Chinese People's Liberation Army in Africa were enrolled in this study.Demographic information,medical history and symptoms were collected for all the patients at admission.Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm or exclude the diagnosis.Data were compared between patients with or without EBOV infection by t test for ages,days from symptoms onset to admission,and x2 test for proportion of symptoms.Results Of 62 patients,35 were male and 27 were female,average age was (27.3 ± 15.4) years.Forty cases were diagnosed with EVD,and 22 were excluded.The incubation period for EVD patients was estimated to be 2 to 24 days with an average of (8.8±5.7) days.EBOV positive patients showed higher proportions of fever,nausea/vomiting,diarrhea,fatigue/weakness,loss of appetite and sore throat than those of EBOV negative patients (all P＜0.05).The proportions of eye pain,hiccup and insomnia were low in EBOV positive patients,while none was in EBOV negative patients.Only one patient had bloody stool at admission.An average of (8 ± 5) symptoms were noted in EVD patients at admission compared with (4±3) symptoms in non-EVD patients (t =8.46,P =0.001).The number of symptoms at admission was optimal in diagnosing EVD with cut-off value of 6.The sensitivity,specificity,positive predictive value and negative predictive value were 50.0％,86.4％,87.0％ and 48.7％,respectively.Thirty-six out of 62 patients had a history of contact with confirmed or suspected EVD cases,including 22 (55.0％) in EBOV positive patients and 14(63.6％) in EBOV negative patients (x2 =0.44,P=0.596).Among 36 cases with definite EVD contact history,19 (86.4％) presented with fever and 20(90.9％) presented with fatigue in EBOV positive patients,while 6 (42.9 ％) presented with fever and 5 (35.7 ％) presented with fatigue in EBOV negative patients (both P＜0.05).Conclusions Among suspected EVD patients,those who are diagnosed with EVD by RT-PCR have more symptoms than those with negative results,including fever,nausea/vomiting,diarrhea,fatigue/weakness,loss of appetite and sore throat,etc.Though these symptoms are not specific,they provide clues for diagnose EVD in the very early stage for clinicians.

Objective To analyze the clinical values of combined detection of procalcitonin(PCT),interleukin-6(IL-6),C-reaction protein(CRP),neutrophil CD64,white blood cell(WBC)count and neutrophil ratio for diagnosis of infection in patients with liver cirrhosis.Methods The hospitalized patients with liver cirrhosis were divided into the infection group and the non-infection group according to the bacterial culture and clinical symptoms.The serum levels of PCT and IL-6 were detected by Chemiluminescence immunoassay.The serum CRP was detected by biochemistry analyzer.The neutrophil CD64 was detected by flow cytometer,and WBC count and neutrophil ratio were detected by blood cell analyzer.The collected data were analyzed by logistic regression and receiver operating characteristic(ROC)curves.Results All the markers in the infection group were higher than those in the non-infection group(P0.01).The results of logistic regression analysis showed that PCT,IL-6and neutrophil CD64 could predict the infection in patients with liver cirrhosis,with the odd ratio being 7.199(95%CI,2.180-23.771),1.010(95%CI,1.002-1.017)and 2.312(95%CI,1.485-3.600),respectively.However,CRP,WBC count and neutrophil ratio showed no predictive values.The ROC curves showed that the area under curves(AUC)of PCT,IL-6and neutrophil CD64 were 0.791(95%CI,0.727-0.856),0.762(95%CI,0.693-0.832)and 0.884(95%CI,0.835-0.933),respectively.The AUC of combined detection of the three markers was 0.932(95%CI,0.897-0.967),with a diagnostic accuracy of 86.9%.Conclusion PCT,IL-6and neutrophil CD64 can predict infection in patients with liver cirrhosis,and combined detection of the three markers can improve the diagnostic efficiency.

Objective To investigate the significance of PCT ,CRP and IL‐6 in the diagnosis and treatment of chronic hepatitis B(CHB) .Methods 60 cases of CHB treated in our hospital from May 2013 to March 2014 were collected as the observation group;at the same time period ,60 individuals with healthy physical examination were se‐lected as the control group .The serum CRP ,PCT and IL‐6 levels were detected and their significance in the diagnosis and treatment of CHB was analyzed .Results The CRP ,PCT ,IL‐6 levels in the observation group were (10 .7 ± 6 .8) mg/L ,(11 .2 ± 6 .1)μg/L and (253 .2 ± 45 .1)ng/L respectively ,which were significantly higher than (3 .3 ± 1 .0) mg/L ,(1 .2 ± 0 .5)μg/L and (64 .5 ± 10 .1)ng/L in the control group ,the differences between the two groups were statistically significant (P<0 .05) .The ROC curve analysis results showed that the sensitivity of CRP was 56 .9%and its specificity w as 70 .6% ;the sensitivity of IL‐6 w as 92 .3% and its specificity w as 95 .5% ;the sensitivity of PCT was 67 .5% and its specificity was 85 .0% .Conclusion The combined detection of PCT ,CRP and IL‐6 has very important clinical value in the diagnosis and treatment of CHB .