Based on the published gene sequence of the N gene sequence of Nigeria 75/ 1strainwas synthesized in GenBank, two pairs of specific primers were designed for amplifying the gene of N1and N5from N gene, connection to the N1and N5gene with Overlap-extension PCR, The sequencing of the N1-N5gene and the expression vector pET-32a(+) by EcoRI and NotI restriction enzyme digestions and inserted into the expression vector pET-32a(+) to construct the recombinant plasmid pET-32a-N1-N5. The recombinant protein was expressed in TransB(DE3) Chemically Competent Cell by IPTG induction and purified.SDS-PAGE showed that the protein was proved to be soluble and Western-blot analysis indicated that the target protein was proved to be immunological activity, The expressed N1-N5protein could be used for the preparation of a rapid surveillance of antibody level of PPRV.