This study aimed to explore the spiritual needs of elderly patients with chronic wounds in China and to provide evidence for developing targeted nursing interventions. A descriptive qualitative design was employed. From February to June 2023, 15 elderly patients with chronic wounds were recruited from a tertiary hospital in Wuxi, China, using purposive sampling, and semi-structured interviews were conducted. Data were analyzed using conventional content analysis. Four major themes were identified: the need for role adaptation and self-worth realization, the need for peace and self-reconciliation, the need for emotional connection and support, and the need for control and hope. These findings indicate that the spiritual needs of elderly patients with chronic wounds are multidimensional and closely intertwined with their physical condition, psychological status, and sociocultural context. Targeted, patient-centered spiritual care strategies, including multidisciplinary collaboration and the use of tele-nursing, may help improve spiritual well-being and overall quality of life.

Background Enteral feeding intolerance (EFI) during enteral nutrition (EN) in adults with severe burn injuries warrants attention. In addition, there is a notable absence of reports from China on this topic. This study is the first to integrate EFI in adult patients with severe burn injuries into a machine-learning model for evaluation and validation. Methods This retrospective cohort study included 195 adults with severe burns. The modeling group comprised 155 eligible patients from the Department of Burns, Affiliated Hospital of Jiangnan University, and the validation group included 40 eligible patients from the Affiliated Hospital of Nantong University. We applied three machine learning algorithms: logistic regression, random forest, and support vector machine. The model performance was assessed using the area under the receiver operating characteristic curve (AUROC). SHapley Additive exPlanations (SHAP) values were used to interpret the model outputs and assess the feature importance. Results Among 195 adult patients with severe burn injuries, 96 (61.94%) were classified as having an EFI. RF achieved the best performance, with AUROC values of 0.992 (95% confidence interval (CI): 0.981–0.999) in the development cohort and 0.895 (95% CI: 0.783–0.997) in the validation cohort. The use of intestinal probiotics and white blood cell count within 24 h of admission were the most influential variables, followed by burn area, fasting blood glucose within 24 h of admission, and the number of underlying diseases. Conclusion The model highlights the key variables in the occurrence of EFI during EN through a SHAP-based interpretation. The use of intestinal probiotics, white blood cell count within 24 h of admission, burn area, fasting blood glucose within 24 h of admission, and the number of underlying diseases were significant contributing factors. Moreover, the final model may provide a basis for individualized EN management strategies for adult patients with severe burn injuries.

In the evolving landscape of nursing education in China, where only 0.2% of clinical nurses hold master’s degrees, upholding professional values among nursing students is crucial in ensuring good patient, nurse, and organization outcomes. Empathy and ethical sensitivity are pivotal in shaping professional values, affecting decision-making, stress management, and nurse-patient relationships. Yet, the underlying mechanisms of empathy and ethical sensitivity in enhancing professional values among nursing master’s students remain underexplored. This cross-sectional study aims to test and compare 2 mediation models with empathy and ethical sensitivity as mediators in affecting professional values among nursing master’s students in China. From September to October 2023, 236 nursing master’s students were selected from a university in Jiangsu Province, China, using convenience sampling. The students’ empathy, ethical sensitivity, and professional values were assessed using the Jefferson Scale of Physician Empathy-Nursing Student (JSPE-NS), the Ethical Sensitivity Questionnaire for Nursing Students (ESQ-NS), and the Nursing Professional Values Scale (NPVS), respectively. Multivariate linear regression analyses were used to assess the effects of empathy and ethical sensitivity on professional values. The mediation models were examined via bootstrapping techniques using SPSS PROCESS v.4.3 macros (Model 4). Findings revealed that the mean scores for empathy, ethical sensitivity, and professional values among nursing master’s students were 104.64 ± 17.28, 39.43 ± 7.71, and 97.99 ± 18.69, respectively. Both empathy (β = 0.385, P < .001) and ethical sensitivity (β = 0.371, P < .001) were independently associated with professional values. Both medication models were statistically significant, and we chose the model that aligns better with established nursing theory and practice: ethical sensitivity partially mediated the relationship between empathy and professional values. The level of professional values among nursing master’s students is moderate. Additionally, ethical sensitivity plays a partial mediating role in the relationship between empathy and professional values. Therefore, enhancing ethical education to improve the ethical sensitivity and empathy of nursing master’s students is essential for fostering the establishment of appropriate professional values.

BackgroundThis study aimed to develop and evaluate SabiWhite-loaded ethosomes (SW-ETH) for topical application, focusing on improving stability, biocompatibility, and therapeutic efficacy. Ethosomal formulations are known for their enhanced drug delivery properties, making them suitable for skin inflammation.MethodsThe SW-ETH formulations were developed utilizing an adapted cold preparation technique. A 3² factorial design was used to optimize phospholipid concentration and ethanol content, and their impact on vesicle size and entrapment efficiency (EE%) was assessed. Structural characterization of SabiWhite was performed using melting point determination, Fourier-Transform Infrared Spectroscopy (FTIR), and X-ray Diffraction (XRD). In vitro drug release was assessed using a Franz diffusion cell, and anti-inflammatory and skin irritation studies were performed on Wistar rats.ResultsSabiWhite exhibited a melting point of 96°C and characteristic FTIR peaks, confirming its identity and purity. XRD analysis revealed its crystalline nature, while ethosomal formulations showed a shift to an amorphous state. The optimized SW-ETH formulation (SW-ETH 6) had a vesicle size of 184.4 nm, an EE% of 92.5%, and a zeta potential of -13.50 mV, indicating stable and uniform vesicles. In-vitro drug release from SW-ETH 6 showed a sustained release profile with 93.12% drug release over 24 hours. In vivo, SW-ETH demonstrated significant anti-inflammatory effects with 36.17% edema inhibition at 150 minutes, comparable to Diclofenac gel (41.92%). No skin irritation was observed, and the formulation was classified as non-irritant. Stability tests confirmed minimal changes in appearance, viscosity, and drug content over 120 days at different storage conditions.ConclusionSW-ETH demonstrated effective drug encapsulation, enhanced anti-inflammatory activity, and excellent biocompatibility, making it a promising candidate for topical therapy. Further clinical validation is warranted to confirm its therapeutic potential.

Diabetes mellitus is a chronic metabolic disorder associated with persistent hyperglycemia, leading to severe complications affecting multiple organs, particularly the liver and wound healing processes. One major factor driving these complications is neutrophil elastase (NE), a protease that plays a pivotal role in inflammation and impaired tissue repair. Despite its crucial involvement, detecting NE activity in vivo has been challenging due to limitations in current detection methods. This study introduces a novel NE-specific probe, NEP, and its self-assembled form, NanoNEP, designed to overcome these challenges. NanoNEP was synthesized by assembling NEP with bovine serum albumin (BSA) to enhance its biocompatibility, solubility, and dispersion. The probe was characterized for its selectivity, stability, and biocompatibility, and demonstrated its ability to monitor NE activity in vivo, specifically in diabetic mice liver, wounds, and serum, as well as in human diabetic foot lesions. These findings highlight the potential of NanoNEP as a sensitive and innovative tool for real-time detection of NE activity, providing valuable insights into the pathogenesis of diabetes-related complications and offering new opportunities for improved diagnostic and therapeutic strategies.

OBJECTIVE:This qualitative study explores the experiences and feelings of older burn patients in China to inform targeted intervention strategies. METHODS:Eighteen older burn patients were purposively sampled for semi-structured interviews, which were transcribed and analyzed thematically. RESULTS:The interview identified four interrelated themes: physiological and psychological dual torment, unmet needs during hospitalization, gains in the rehabilitation process, and concerns and aspirations for future life. Specifically, we identified some findings that are unique to a Chinese socio-cultural context, including the unmet privacy needs of wound exposure, the positive gains and appreciation for their family members' care and support, as well as the concerns about imposing caregiver burden on their adult children. CONCLUSIONS:Older burn patients face both physical and psychological challenges, with unmet service needs and limited access to resources. This study offers valuable insights for developing integrated intervention strategies to improve care and health outcomes for this population.

Background: Excessive inflammation and fibrosis during wound healing can cause delayed repair and pathological scar formation. This study aimed to investigate the effects of butorphanol on inflammation and fibrosis during wound healing and to explore the underlying molecular mechanisms. Methods: A rat burn wound model was established and treated with varying doses of butorphanol. Wound closure rates were recorded on days 3, 7, 10, and 14 post-injury. Histological analyses were performed to assess tissue repair and fibrosis. Expression levels of pro-inflammatory cytokines, macrophage polarization markers, fibrosis-related proteins, and p38/c-Jun N-terminal kinase (JNK) signaling molecules were measured using real-time quantitative polymerase chain reaction (RT-qPCR), immunohistochemistry, and Western blotting. Pharmacological inhibitors and agonists targeting the interleukin-6 (IL-6)/p38/JNK pathway were applied to validate mechanistic involvement. Results: Butorphanol significantly accelerated wound healing, reduced inflammatory cell infiltration, and downregulated pro-inflammatory cytokines in the early stages (p < 0.05). It promoted macrophage polarization toward the anti-inflammatory M2 phenotype by increasing Cluster of Differentiation (CD)206 expression and suppressing CD86 expression (p < 0.05). Temporally, butorphanol enhanced the early expression of fibrosis-associated markers to support the transition to the proliferative phase, while suppressing their excessive late-stage expression to prevent pathological scarring (p < 0.05). Mechanistically, butorphanol inhibited activation of the IL-6/p38/JNK signaling pathway, as evidenced by altered pathway activity following pharmacological modulation, thereby regulating inflammation and fibrosis during healing (p < 0.05). Conclusion: Butorphanol promotes orderly wound healing by modulating inflammation and fibrosis through regulation of the IL-6/p38/JNK signaling axis. These findings establish butorphanol as a promising therapeutic candidate for enhancing burn wound repair, and offer new perspectives on the IL-6/p38/JNK axis as a potential molecular target for regulating inflammation and fibrosis.

High temperature-induced burn injury often leads to an excessive inflammatory cascade resulting in multiple organ dysfunction syndrome, such as acute lung injury (ALI), in addition to skin tissue damage. As a specific COX2 inhibitor, parecoxib sodium suppresses the inflammatory response during burn injury. The effect of parecoxib sodium on ALI induced by burn injury and the associated molecular mechanism still need to be investigated. The role of parecoxib sodium in burn injury-induced ALI through the TLR4/NF-xB pathway was explored in the present study. A burn-induced ALI mouse model was constructed, and M1/M2 macrophages in lung tissue and markers involved in the TLR4/NF-xB signalling pathway were evaluated in bronchoalveolar lavage fluid (BALF) and MH-S mouse alveolar macrophages in vitro. The results indicated that parecoxib sodium attenuated lung injury after burn injury, decreased iNOS and TNF-a expression, increased IL-10 expression in BALF, and regulated the CD86-and CD206-mediated polarization of M1/M2 macrophages in lung tissue along with MH-S mouse alveolar macrophages. The effect of parecoxib sodium might be reversed by a TLR4 agonist. Overall, the results suggested that parecoxib sodium can regulate the polarization of M1/M2 macrophages through the TLR4/ NF-xB pathway to attenuate ALI induced by skin burns.

Purpose: The purpose of this study was to examine how fatalism acts as a mediator in the correlation between family resilience and self-management among patients with chronic wounds in China. Participants and Methods: This study used a cross-sectional research design. A total of 269 adult patients (18-94 years old) with chronic wounds residing in Wuxi, China participated in this study. Participants completed the Chinese version of the Walsh Family Resilience Questionnaire, 16-item Chinese version of the Fatalism Scale, and Self-Management Scale of Chronic Wound Patients. We conducted correlation and mediation analyses using SPSS 27.0 and PROCESS 4.0. Results: The results indicated family resilience was a significant positive predictor of self-management (beta = 0.7101, p < 0.0001), and the pathway between family resilience and self-management was partially mediated by fatalism (Effect = 0.1432, 95% confidence interval [0.0625, 0.2341]).Conclusion: The results indicated that incorporating spiritual interventions into future person-centered self-management programs could align with the motivation of patients with chronic wounds and their families, and reduce the negative impact of fatalism on health outcomes.

High temperature-induced burn injury often leads to an excessive inflammatory cascade resulting in multiple organ dysfunction syndrome, such as acute lung injury (ALI), in addition to skin tissue damage. As a specific COX2 inhibitor, parecoxib sodium suppresses the inflammatory response during burn injury. The effect of parecoxib sodium on ALI induced by burn injury and the associated molecular mechanism still need to be investigated. The role of parecoxib sodium in burn injury-induced ALI through the TLR4/NF-κB pathway was explored in the present study. A burn-induced ALI mouse model was constructed, and M1/M2 macrophages in lung tissue and markers involved in the TLR4/NF-κB signalling pathway were evaluated in bronchoalveolar lavage fluid (BALF) and MH-S mouse alveolar macrophages in vitro. The results indicated that parecoxib sodium attenuated lung injury after burn injury, decreased iNOS and TNF-α expression, increased IL-10 expression in BALF, and regulated the CD86−and CD206-mediated polarization of M1/M2 macrophages in lung tissue along with MH-S mouse alveolar macrophages. The effect of parecoxib sodium might be reversed by a TLR4 agonist. Overall, the results suggested that parecoxib sodium can regulate the polarization of M1/M2 macrophages through the TLR4/NF-κB pathway to attenuate ALI induced by skin burns.

A triple-layer matrix Collagen/Silk fibroin/Bioactive glass composited Nanofibrous was fabricated by linking electrospinning and freeze-drying systems, this typical three layered composite with a nanofibrous fragment as the key (top) layer, middle portion as inferior, and a spongy porous fragment as the third (bottom) deposit to develop the synergistic effect of composite materials resultant to physical and biological performances. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy were used to assess the final material's physicochemical properties (SEM). The triple-layer matrix had a nanofibrous and porous structure, which has qualities including high porosity, swelling, and stability, which are important in soft-tissue engineering. NIH 3 T3 fibroblast and humanoid keratinocyte (HaCaT) cell lines were also used to investigate the matrix's in vitro biological and fluorescent capabilities, which showed excellent cell adherence and proliferation across the composite layers. The synergistic arrangement of nanofibrous substantial deposition onto collagenous with silk fibroin candidates has therefore proven effective in the construction of a tri-layer matrix for skin-tissue-engineering applications.

Objective:To investigate the surgical treatment methods of femoral artery pseudoaneurysm combined with infectious wounds and to evaluate the clinical effects.Methods:The retrospective observational research method was used. Twelve patients with femoral artery pseudoaneurysm combined with infectious wounds who met the inclusion criteria were admitted to Nanjing University of Chinese Medicine Wuxi Integrated Traditional Chinese and Western Medicine Hospital (Affiliated Hospital of Jiangnan University) from October 2014 to September 2022, including 6 males and 6 females, aged from 46 to 78 years. In the primary operation, debridement, tumor resection, and artery suture/venous grafting to repair the artery/artery ligation were performed, and the wound area after tumor resection ranged from 4.0 cm×1.5 cm to 12.0 cm×6.5 cm. Wounds that could be sutured were treated with tension reduction suture and extracutaneous continuous vacuum sealing drainage (VSD), while large wounds that could not be sutured were treated with VSD to control infection. In the secondary operation, tension reduction suture was performed to repair the wounds that could be sutured; large wounds were repaired with adjacent translocated flaps with area of 9.0 cm×5.0 cm to 15.0 cm×7.0 cm. Additionally, when the length of the exposed femoral artery was equal to or over 3.0 cm, the wounds were repaired with additional rectus femoris muscle flap with length of 15.0 to 18.0 cm. The donor areas of the flaps were directly sutured. The wound with artery ligation was treated with stamp skin grafting and continuous VSD. The bacterial culture results of the wound exudate samples on admission were recorded. The intraoperative blood loss, the location of femoral artery rupture, the artery treatment method, and the wound repair method in the primary operation were recorded, and the durations of catheter lavage, catheter drainage, and VSD treatment, and the drainage volume after the operation were recorded. The repair method of wounds in the secondary operation, the durations of catheter drainage and VSD treatment, and the total drainage volume after the operation were recorded. The survivals of flap/muscle flap/stamp skin grafts were observed, and the wound healing time was recorded. Follow-up after discharge was performed to evaluate the quality of wound healing and the walking function and to check whether the pulsatile mass disappeared. B-ultrasound or computed tomography angiography (CTA) was performed again to observe potential pseudoaneurysm recurrence and evaluate the patency of blood flow of the femoral artery.Results:The bacterial culture results of wound exudate samples of all the patients were positive on admission. The blood loss was 150 to 750 mL in the primary operation. The arterial ruptures were located in the femoral artery in 8 cases, in the external iliac artery in 2 cases, and in the femoral arteriovenous fistula in 2 cases. Six cases received direct artery suture, 4 cases received autologous great saphenous vein grafting to repair the artery, 1 case received autologous great saphenous vein bypass surgery, and 1 case received artery ligation. The primary wound suture was performed in 4 cases, along with catheter lavage for 3 to 5 days, catheter drainage for 4 to 6 days, VSD treatment for 5 to 7 days, and a total drainage volume of 80 to 450 mL after the surgery. In the secondary operation, the wounds were sutured directly in 3 cases along with catheter drainage for 2 to 3 days, the wound was repaired with scalp stamp skin graft and VSD treatment for 5 days in 1 case, the wounds were repaired with adjacent translocated flaps in 2 cases with catheter drainage for 2 to 3 days, and the wounds were repaired with rectus femoris muscle flaps+adjacent translocated flaps in 2 cases with catheter drainage for 3 to 5 days . The total drainage volume after the secondary operation ranged from 150 to 400 mL. All the skin flaps/muscle flaps/skin grafts survived after operation. The wound healing time ranged from 15 to 36 days after the primary operation. Follow-up of 2 to 8 months after discharge showed that the wounds of all patients healed well. One patient who underwent femoral artery ligation had calf amputation due to foot ischemic necrosis, and the rest of the patients regained normal walking ability. The pulsatile mass disappeared in inguinal region of all patients. B-ultrasound or CTA re-examination in 6 patients showed that the blood flow of femoral artery had good patency, and there was no pseudoaneurysm recurrence.Conclusions:Early debridement, tumor resection, and individualized artery treatment should be performed in patients with femoral artery pseudoaneurysm combined with infected wounds. Besides, proper drainage and personalized repair strategy should be conducted according to the wound condition to achieve a good outcome.

BACKGROUND:Small-area burn is a common but specific type of injury that can still lead to serious complications if not managed properly. Researchers have introduced a number of interventions.OBJECTIVE:The objective of this study was to compare the effects of eCASH concept-based care (ie, eBCare) with those of standard treatment on wound healing in patients with small-area burns.METHODS:In this prospective randomized controlled trial, patients with small-area burns received either eBCare (n = 35) or standard burn treatment (n = 35) for 2 weeks. Pain, anxiety, heart rate, exudate, and wound area were measured during and after dressing changes. Scar color and thickness were assessed 4 years or longer after discharge.RESULTS:The eBCare group had a lower median pain score, anxiety score, and heart rate compared with the control group (P < .001). The eBCare group also had a higher median wound healing rate on day 14 than the control group (P < .05). At follow-up 4 years or more after discharge, the eBCare group had better scar color and thickness than the control group (P < .05).CONCLUSION:The use of eBCare reduced pain and anxiety, accelerated wound healing, and improved scar outcomes in patients with small-area burns, which suggests that eBCare may be a feasible option for this population.

慢性伤口疼痛在大于65岁的老年人中尤其常见,并严重影响老年人生活质量,但目前却没有得到足够的重视.随着慢性疼痛分类的规范和大数据调查的展开,慢性疼痛的规范化管理也进入一个新时期.简易的GCPS-R量表、先进的通过镇痛、最小镇静药物和最大人文关怀获得早期舒适(eCASH)理念和管理策略都为医护人员治疗老年慢性伤口疼痛提供了新的借鉴.eCASH理念具有广泛适用性和人文科学性,对老年慢性伤口疼痛的管理具有重要参考意义.本文就老年慢性创面疼痛的特点、影响、新近评估和治疗方法进行综述,旨在为老年人慢性伤口的疼痛管理提供新的思路.

Nerve injury leads to the accumulation of white blood cells derived from the bone marrow in the lesioned nerve, but it is still unknown whether there are similar responses in unlesioned nerves. To address this question, sciatic nerves of mice expressing enhanced green fluorescent protein (EGFP) in their bone marrow were crushed unilaterally to observe the invasion of bone marrow-derived cells into the contralateral unlesioned nerve. Two days after surgery, EGFP+ cells began to infiltrate both the damaged and undamaged nerves. These cells gradually amplified to the highest point within 14 days and slowly lowered. In ipsilateral (lesioned) and contralateral (unlesioned) nerves, the time course of infiltration of EGFP+ cells was similar, but the magnitude was much less for the unlesioned one. Through CD68 staining, some cells were identified as macrophages. Transmission electron microscopy revealed slight demyelination and phagocytosing macrophages in the contralateral nerve. The data showed that infiltration by white blood cells is a response to nerve injury, even in uninjured nerves.

Objective:To investigate the clinical efficacy of abdominal multiple wide pedicled ultra-thin flaps for repair of multiple finger wounds.Methods:From January 2016 to December 2020, 20 patients (51 fingers) with multi finger wounds were treated, all of which were volar or dorsal wounds of unilateral multi finger hands. There were 8 cases of volar digital wounds and 12 cases of dorsal digital wounds. Among them, 11 cases had wounds in 3 fingers and 9 cases had wounds in 2 fingers. The single wound area ranged from 1.0 cm×2.0 cm to 3.0 cm×7.0 cm. The ipsilateral abdominal multiple wide pedicled ultra-thin flaps were used to repair the wound of multiple fingers, and the pedicle was broken in 14 to 18 days.Results:After operation, one of the flaps was cured after dressing change because of large suture tension and partial necrosis of the distal end. The flaps of other cases survived completely. The flap was follow-up for 3 to 20 months. The appearance of the flap was not bulky and the texture was good. According to the evaluation standard of hand tendon repair issued by Hand Surgery Society of Chinese Medical Association, the results were rated as excellent in 35 fingers, good in 9 fingers, fair in 5 fingers and poor in 2 fingers.Conclusion:The application of abdominal multiple wide pedicled ultra-thin flaps can repair the wound of multiple fingers at the same time. The survival rate of the flap is high, the appearance and texture are good, and the recovery of hand function is satisfactory.

Given the new ideas on wound care offered by the eCASH (early Comfort using Analgesia, minimal Sedatives, and maximal Humane care) and the substantial differences in clinical treatment between acute and chronic wounds, we aimed to investigate the effect of comfort therapy under the eCASH concept on analgesic sedation and accelerated wound healing in patients with acute or chronic wounds. This randomized clinical study was conducted in two parts: acute wounds and chronic wounds. Patients with acute wounds were allocated into the acute wound control group (AWCG) and the acute wound experimental group (AWEG). Patients with chronic wounds were allocated into the chronic wound control group (CWCG) and two experimental groups, in which they received intermittent negative pressure therapy (IPTEG) and continuous negative pressure therapy (CPTEG). On the basis of the standard treatment for patients in the control group, eCASH therapy was used in the experimental groups. In addition, pain intensity and procedural anxiety were evaluated using the visual analogue score (VAS) and the Hamilton Anxiety Scale (HAM-A). In addition, clinical effects were assessed on the basis of the size of the surface area, rate of healing, and concentration of pro-inflammatory factors (IL-1, IL-6, TNF-α) and growth factors (VEGF, bFGF, TGF-β1). Compared with the control group, the VAS score and HAM-A score in the experimental groups were significantly decreased after intervention (P < 0.05). After intervention, the levels of IL-1β, IL-6, and TNF-α in AWEG, IPTEG, and CPTEG were significantly lower than those in AWCG. In addition, the levels of VEGF, bFGF, and TGF-β1 in IPTEG and CPTEG were significantly higher than those in CWCG (P < 0.05). These results indicated that comfort therapy under the eCASH concept has a significant effect on ameliorating the pain and anxiety of patients, reducing the inflammatory reaction during the period of wound healing in the treatment of acute and chronic wounds. The trial has been registered in the Chinese Clinical Trial Registry (ChiCTR2200057981).

A triple‐layer matrix Collagen/Silk fibroin/Bioactive glass composited Nanofibrous was fabricated by linking electrospinning and freeze‐drying systems, this typical three layered composite with a nanofibrous fragment as the key (top) layer, middle portion as inferior, and a spongy porous fragment as the third (bottom) deposit to develop the synergistic effect of composite materials resultant to physical and biological performances. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy were used to assess the final material's physicochemical properties (SEM). The triple‐layer matrix had a nanofibrous and porous structure, which has qualities including high porosity, swelling, and stability, which are important in soft‐tissue engineering. NIH 3 T3 fibroblast and humanoid keratinocyte (HaCaT) cell lines were also used to investigate the matrix's in vitro biological and fluorescent capabilities, which showed excellent cell adherence and proliferation across the composite layers. The synergistic arrangement of nanofibrous substantial deposition onto collagenous with silk fibroin candidates has therefore proven effective in the construction of a tri‐layer matrix for skin‐tissue‐engineering applications.

目的:比较瘢痕内注射A型肉毒毒素和曲安奈德的疗效及副作用.方法:选取2020年6月-2021年1月笔者医院整形美容科就诊的40例增生性瘢痕患者为研究对象,依据随机数字表法将患者分为两组,每组20例.对照组局部注射曲安奈德治疗,观察组局部注射A型肉毒毒素治疗,比较两组患者治疗后满意度和不良反应发生率,采用改良温哥华瘢痕量表(VSS)评估治疗前后增生性瘢痕的变化,采用视觉模拟评分表(VAS)评估治疗前后瘢痕处痛痒觉变化,采用CBS瘢痕检测仪评估治疗前后瘢痕处皮肤特性(瘢痕面积、色素含量、水分含量及油分含量)变化.结果:观察组患者总满意度为80.00％,显著高于对照组的45.00％,组间差异有统计学意义(P<0.05);对照组不良反应发生率为20.00％,显著高于观察组的0.00％,组间差异有统计学意义(χ2=4.444,P<0.05).治疗后,两组VSS分值及VAS均较治疗前降低,差异均有统计学意义(P<0.05).治疗后两组VSS、VAS改善程度比较,差异无统计学意义(P>0.05).结论:曲安奈德和A型肉毒毒素注射液治疗增生性瘢痕,其临床疗效未见明显差异,但肉毒毒素治疗不良反应发生率低,副作用少,患者满意度高,安全性更高,可临床推广应用.