Objective To investigate the method for the determination of concentration of erucic acid methyl ester in human hepatocellular line BEL-7404.Methods The concentration of erucic acid methyl ester in the blank cell lysate,the medium and the lysate of cells that added erucic acid methyl ester were detected by thin layer chromatography-gas chromatography(TLC-GC),and the relationship between the concentration of erucic acid methyl ester and the peak area.Results A good linear correlation between peak area and concentration were obtained within a concentration range of 50~5 000 μg/ml(r=0.999 95).The amount of erucic acid methyl ester microemulsion got into human hepatocellular line BEL-7404 were 24.9%.Conclusions TLC-GC can determine the concentration of the drug in the cell accurately;this method is simple,rapid and stability.

Isopropyl erucate was prepared by the reaction of erucic acid with isopropyl alcohol in concentrated sulfuric acid in microscale organic lab and purified with column chromatography. Its structure was characterized by IR and ~1H-NMR.The result showed that isopropyl erucate was synthetized and identified.Unsaturated fatty acids can be synthetized quickly and conveniently with microscale organic synthesis technique.