In order to confirm genetic stability and early fattening expression level difference of foreign porcine growth hormone gene(pGH) in F1 generation of transgenic pigs, growth hormone in 7 trans-pGH gene pigs and 9 non-transgenic pigs of 3-month-old were detected by the advantage of SYBR GreenⅠReal-time PCR method, which were produced by F0 transgenic sows made by nano-gene carrier method induced by sperm and normal boar. According to amplification curve and melting curve maps, Ct mean value was gained through the equation F=(1+E)-ΔΔCt. The results showed that pGH average expression level in F1 generation of transgenic pigs was 1.77 times as non-transgenic pigs. Suggesting that: 1) foreign gene pGH could stably passage in transgenic pigs, 2) not every pGH gene's expression level in transgenic pig was higher than that of non-transgenic pig, 3) average expression level of pGH gene in F1 generation transgenic pigs was observably higher than that of non-transgenic pigs in the same period. A set of molecule biology method of evaluating pGH relative expression level stably was built, and provided scientific method for discussing physical signs and quantitative expression between transgenic and non-transgenic animals.

Heat shock protein 70(HSP70) gene was regarded as a candidate gene for thermo resistance of cows.In this study,a 549 bp fragment of HSP70 gene of Chinese Holstein was amplified by polymerase chain reaction(PCR),the polymorphisms of three loci of HSP70 gene were detected by PCR-SSCP technique.By sequencing,three point mutations,G/C at nucleotide 6 400,C/T at nucleotide 6 600 and G/A at nucleotide 6 601,were identified in HSP70 gene sequence.χ2 test indicated that three polymorphisms in the population unfitted with Hardy-Weinberg equilibrium(P 0.01).Meanwhile,the relationship between milk performance and heat tolerance was analyzed.The results showed that in the three loci,the different genotypes of HSP70 gene had no significant influence on milk protein percent and fat percent,but had significant influence on 305-day milk yield.Individuals with genotype BB showed higher 305-day milk yield than those with genotypes AA and CC(P 0.05).And the cows with genotype BB showed lower potassium content in erythrocytes(PCE) and decrease rate of milk yield than those with genotypes AA and CC.It indicated that BB genotype was the advantageous genotype.These mutations could be used in MAS for anti-heat stress cows in our breeding program.