应用SSU rDNA和线粒体cox 1序列研究甲藻（Dinoflagellate）中不同生态类群的演化关系。通过PCR扩增和测序获取4株甲藻SSU rDNA及其线粒体cox 1部分片段,结合GenBank中24株甲藻的相关序列,以Plasmodium falciparum为外群,构建ML树和NJ树,并采用自展支持度评估进化树分支结构,通过计算后验概率评估进化树整体结构,应用1sKH、SH、ELW和2sKH等方法评估两株ML树间的拓扑结构。结果显示,在由上述两序列构建的进化树上,底栖原甲藻均未与浮游原甲藻聚类,且前沟藻具有独特演化地位。表明联合选择SSU rDNA和线粒体cox 1序列构建的进化树在一定程度上能够反映出甲藻的演化规律。这为从基因层面深入探索甲藻中不同生态类群的演化关系初步奠定了基础。

DNA extraction of red tide-forming causative species Amphidinium sp.was performed.PCR technology was used to amplify 18S rDNA.The result of amplification indicated that the length of PCR productions of Amphidinium sp.was 1 736 bp,G+C was 47%.Then,homology analysis was performed using BLAST in NCBI,and the result indicated that the fragment of 18S rDNA of Amphidinium sp.we cloned had a high homology(99%)with that of Amphidinium carterae,95% homology with any other Amphidinium sp.in GenBank,85% homology with Gymnodinium and Prorocentrum.There was no distinct homology with human genomic and mouse genomic.The results show that,18S rDNA was an effective method for rapid identification of genus,while not quite sure for the species identification.

Gene chip usually refers to DNA chip.Its basic principle is that a large number of oligonucleotide molecules fixed on the support material were first hybridized with the labeled sample,and then the results can be acquired by measuring the hybridization signal.Over the years,a variety of gene chips in medicine,microbial detection,has been successfully developed in China and many impressive achievements have been obtained.However,the application of gene chips in marine microalgae has been few reported.This article introduced the application and perspective of gene chip in marine microalgae.