To prepare standard antigen and polyclonal antibodies for detection of chicken egg drop syndrome-76 virus ( EDS-76) , we developed a rapid method of virus purification with international strain AV-127. Our results showed that the purified virus particles could be obtained by chloroform extraction and PEG concentration from allantoic fluid. The HA titer of the purified virus reached to 19Log2. The polyclonal antibodies to EDS-76 generated here showed 13Log2 in HI titer and 1 ∶ 2000 in IFA titer. Western-blot analysis results showed the same pattern to the available commercial sera against EDS-76. Moreover, the generated polyclonal antibodies against EDS-76 did not show cross-reaction with 12 other avian pathogens( NDV, AIV-A, ALV-J, ALV, GPV, REV, MDV, IBV, CAV, IBDV, TMUV, REOV) . Overall, our rapid viral purification method provides efficient tools and materials in developing standard antigen and antibodies for detection of EDS-76 in future.

To investigate the biological function of UL14 protein during the course of MDV infection,UL14 gene from CEF cells infected with RB1B strain was amplified by PCR.UL14 gene was cloned into pET32a(+) vector and expressed in E.coli BL21(DE3).Recombinant His-UL14 protein expressed in E.coli was soluble.The BALB/c mice were immunized with recombinant UL14 protein and resulting antiserum was used in IFA.The specific inflorescence was visualized in CEF cells infected with MDV strain RB1B.