Objective To establish a method of cultivation and purification of dendritic cells(DCs) from mouse bone marrow and spleen in vitro and provide experimental material and establish basis for the study of immunological tolerance.Methods Extracted the ICR mice spleen lymphocytes and bone marrow cells under sterile conditions,and cultured DCs in co-induced of recombinant granulocyte-macrophage colony-stimulating factor(GM-CSF) and interleukin4(IL-4).Then,the changes of DC morphology were observed under light microscopy and CD80,CD86 expression levels were detected with flow cytometry.Results bone marrow cells appeared irregular and formed dendritic processes after 3 days cultured with stimulating factor in vitro which showed the typical morphology of dendritic cells,and costimulatory molecules(CD80,CD86) were low expression on the cellular suface.Conclusion Compared with the spleen cells,bone marrow cells are not only rich in DC precursor cells but also induced into DCs with a short time.

目的:探讨Nuss手术矫正漏斗胸的优越性。方法:实施漏斗胸矫形术共31例。其中10例使用胸腔镜,21例未使用胸腔镜;2例使用固定片,29例使用钢丝固定。结果:手术过程顺利,手术时间为60~90 m in,术中出血量5~15 m l,术后平均9~12 d出院,无并发症发生。出院后随诊2年,30例患者漏斗胸矫形满意,1例术后复发。矫形成功的患者中,有2例钢丝折断。结论:Nuss手术具有切口小而隐蔽、手术时间短、手术创伤小、出血少、活动早、无手术瘢痕、矫形效果好等优点,使用胸腔镜可确保术程清晰可示,手术方法安全可行,值得推广。

1 病历摘要 患者,女,52岁.1月前无明显诱因出现胸闷、胸痛,15天前于当地医院就诊,行肺部CT检查提示右肺巨大肿块,右侧胸腔积液,行胸腔穿刺引流术,引流出黄色液体约500 ml,症状未见好转,遂来我院就诊,门诊以右侧胸腔巨大肿物收入院.病程中未见咳嗽、咳痰,无发热、盗汗,二便如常,体重减轻2 kg.患者既往高血压病史10年,规律月侧用降压药血压控制尚可.

<正>2010年1月和7月,我院成功对2例复杂的气管隆突癌实施了外科手术,术后恢复良好,现报告如下。1病历摘要1.1患者,男,59岁,住院号736112,以哮喘6个月在中国内蒙古通辽市医院就诊,经检查诊断为气管(中下段)隆突癌,组织类型为基底细胞癌

To study the role of CD4 + CD25 + Treg cells in anti-graft rejection response and potential value in application of lung transplatation, we established mice trachea allograft transplant model and sort CD4 + CD25 + Treg cells from mice spleen lymphocytes by MACS. FCM assays were done to analyze the ratio of marker protein, Foxp3, expression in CD4 + CD25 + Treg cells. Alveolar cells were used as a non-specific antigen to activate iDCs into mDCs, and cocultureed with CD4 + CD25 + Treg cells to activate Treg cells. The activated Treg cells were inject into mice with trachea allograft transplantation to reduce rejection response. The allografts were extracted and done pathological examination with H&E staining. The purity of CD4 + CD25 + Treg cells were over 80% and specifically express Foxp3, the organizational structure of implanted trachea showed that the tracheal structure was intact. Our research imply that CD4 + CD25 + Treg cells play an essential role in reducing rejection, and lay the foundation for reject anti-graft response in clinical application.

Objective To analyze the effect of T2~T3 sympathicotomy in the treatment of primary palmar hyperhidrosis.Method Total 25 patients with primary palmar hyperhidrosis,including 12 males and 13 females,with the average age of 30.7 years,underwent T2~T3 sympathicotomy,in which the sympathetic chains were transected at the level of the third ribs.Results Monitoring the skin temperature of both palm.The skin temperatures of the right palm were(32.0±1.6)℃ preopratively and(34.5±1.3)℃ postoperatively(P0.05).The skin temperatures of the left palm were(32.6±1.5)℃ preopratively and(35.0±1.3)℃ postoperatively(P0.05).The procedures were successful.There were no severe intro-and post-operative complications or mortality.One case presented the Horner syndrome.All cases were followed for 6~29 months,average 16.5 months,presenting an effective rate of 100%.Conclusion Sympathicotomy is a simplified method with little side effect and higher satisfactory rate.It should be recommended for the treatment of primary palmar hyperhidrosis.

Establish a method of cultivation and purification of dendritic cells (DCs) from mouse bone marrow in vitro to provide experimental material and establish basis for the study of immunological tolerance. BALB/c (H-2d) mice spleen lymphocytes and bone marrow cells were extracted under sterile conditions and cultured in RPMI 1640 medium. DCs were induced by recombinant granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin (IL-4). Then, the morphology of DCs was observed under light microscopy and CD80, CD86 expression levels were detected by flow cytometry. The results showed that bone marrow cells appeared irregular and formed dendritic processes after 3 days cultured with stimulating factors in vitro which present the typical morphology of dendritic cells; Costimulatory molecules (CD80, CD86) were low expression on the cellular surface. Obtaining DCs from bone marrow cells is a promising method, which might meet further study on function, marker and anti-tumor role of DCs in vitro.

目的:比较电视胸腔镜技术(VATS)下肺叶切除术与开胸肺叶切除术的临床治疗效果.方法:随机选取施行VATS下肺叶切除术及开胸肺叶切除术各30例.比较两组患者的手术时间、术中出血量、引流管留置时间、术后拆线时间,术后住院时间.结果:VATS组手术时间100～410 min,平均191.4 min;术中出血量50～500 ml,平均211.67 ml;引流管留置时间2～15 d,平均6.03 d;术后拆线时间8～12 d,平均9.6 d;术后平均住院时间8.28 d.开胸组手术时间90～230 min,平均156.06 min;术中出血量100～1 000 ml,平均277.67 ml;引流管留置时间3～15 d,平均7.86 d;术后拆线时间9～20 d,平均10.6 d;术后平均住院时间10.83 d.两组五项指标对比,差异有统计学意义(P<0.05).结论:与开胸肺叶切除术相比,VATS下肺叶切除术具有创伤小、术后恢复快等优点.随着VATS下肺叶切除术技术的不断进步,VATS下肺叶切除术将成为治疗肺恶性肿瘤一项重要乃至主要手术术式.

Objective Investigate the expression of Survivin in esophageal carcinoma and its relationship with clinical pathological parameter.Methods Examine the expression of Survivin in 35 cases of esophagus cancer,5 cases of normal esophagus tissue and 12 cases of adjacent tissue of esophageal carcinoma with imunohistochemistry.Results The 35 specimen in esophagus cancer tissue appeared Survivin positive expression,and only 1 specimen in normal esophagus tissue and adjacent tissue present Survivin weakly positive expression.The expression of Survivin had no relationship with histological type.There is significant difference between the average optical density of the cancer tissue and of the normal tissue(P0.05),while there is no significant difference between the average optical density of the lateral cancer tissue and of the normal tissue(P0.05).Conclusion The apoptosis inhibition caused by Survivin protein may play an essential role in the origin and progression of esophagus cancer.

Objective To explore the effect of Heller improved operation in the treatement of achalasia of the cardia.Method Total 50 patients with achalasia of the cardia,17 males,33 females,average age of 30.7 years(2~73 years).Heller improved operation and intravenous anesthesia with tracheal intubation were used.Results For 50 cases′ operations were success and none of them appeared esophageal rupture,the recovery rate was 99.60% and without pleural effusion.Conclusion Heller improved operation is a simplified method for achalasia of the cardia with fewer side effects and higher satisfactory rate.It is effective than conventional surgery.So this is a recommended surgical method of operation.