The objective of this study was to establish a loop-mediated isothermal amplification(LAMP) method for the detection of orf virus(OrfV).According to OrfV sequence available in GenBank,a set of four primers were designed based on the OrfV B2L gene sequence.Optimal conditions,specificity test and sensitivity test were performed.In result,ladder-like products were amplified from the OrfV-positive samples by LAMP,while no product was generated from goatpox virus,sheeppox virus,bluetongue virus,foot-and-mouth disease virus,and contagisas caprine pleuropneumonia.The LAMP assay had a detection limit equivalent to 5.3 fg of OrfV,which was 1 000 times more sensitive than PCR method.Tests of 6 clinical samples by the assay showed OrfV-positive rate of 83.33%(5/6).The agreement rate between LAMP and PCR was 100% in detection clinical samples.In conclusion,the developed LAMP is a novel method for the rapid detection of OrfV.

<正>猪直肠脱出俗称"脱肛",是直肠末端黏膜或直肠后侧全层垂脱到肛门外而不能自行复位。常因大便秘结、直肠炎、刺激性药物灌肠、顽固性腹泻、母猪难产、中气下陷、肛门括约肌弛缓或突然改变饲料和维生素缺乏等引起。本病多发于2～4月龄小猪、体弱的架子