Objective To establish a qualitative and quantitative determination of glyphosate in serum using ultraviolet spectro-photometry(UV) to provide basis for clinical diagnosing and treating glyphosate poisoning .Methods The mixture of 0 .5 mL serum and 0 .2 mL 10% methanol solution of perchloric acid was shocked and centrifuged with 10 000 r/min for 5 min .A nitrosyla-tion reaction conducted on supernatant and 50 μL serum nitrosylation liquid was detected by UV scanning .Results The results of serum theophylline absorption maxima was(243 ± l) nm and the concentration of 10 .0-60 .0 μg/mL range linear regression equa-tion was Y=0 .0173 8X+0 .036 3(r= 0 .999 8) .The recovery rate was from 85 .5% to 102 .4% and the relative standard deviation (RSD) was from 3 .50% to 4 .90% .The intra-day and inter-day RSD were 3 .79% -5 .10% and 3 .88% -4 .55% .The minimum de-tectable concentration was 5μg/mL .Conclusion This method is simple ,rapid and accurate results for detecting glyphosate poison-ing .

患者,男,61岁,农民.因咽痛伴吞咽困难及双侧颌下肿痛3个月余,于2008年6月10日来我院就诊.

Objective To realize the simultaneous control of two laboratory instruments by a computer. Methods On the basis of analysing the mode of communication between computer and laboratory instrument, the author expanded the computer's COM interface through the USB / COM converter. Results The author realized a computer controlling MP280 CLIA chemiluminescence analyzer and anthos 2010 enzyme tester at the same time. Conclusion User can use computer's USB interface and USB / COM converter to achieve controlling multiple instruments and equipments at the same time, as long as the communication interface of equipment is open. This method has preferable potential application.

重症急性呼吸综合征(SARS)的病原体相继被不同实验室发现并确证为一种新型冠状病毒,又称为SARS病毒[1],已确定了病毒基因的一级结构和一些基因组特征,为利用分子免疫学手段研究和建立SARS的血清学诊断方法奠定了基础[2].我国军事医学科学院等单位已成功建立了检测SARS病毒抗体的ELISA方法,并开发出相应的检测试剂盒.本研究采用ELISA方法检测SARS患者和医务人员血清SARS病毒特异IgG抗体,观察SARS病毒抗体在患者和高危易感人群的产生规律和水平,为SARS的诊断及其流行传播特点的分析提供实验依据.