Objective:To investigate the pathogenic characteristics of viral encephalitis or meningitis in children living in Shijiazhuang city and surrounding areas, and to study the relationship between pathogenic and clinical findings.Methods:A total of 132 cerebrospinal fluid specimens were randomly collected from hospitalized children diagnosed with viral encephalitis or meningitis (January 2018 to December 2018) in the Department of Neurology of Hebei Children′s Hospital in Shijiazhuang city and surrounding areas. The nucleic acids of four viruses in cerebrospinal fluid were detected by real-time quantitative PCR. SPSS 21.0 software was used for statistical analysis.Results:Among the 132 cases, 78 were boys and 54 were girls, with a sex ratio of 1.44∶1. However, in the gender composition of children in each age group, there was no significant difference (χ 2=3.901, P=0.272). Of the 132 children, 121 had signs of fever, 109 had symptoms of headache, 92 had symptoms of vomiting, 17 had abnormal electroencephalogram(EEG), and 15 had abnormal head magnetic resonance imaging(MRI). 132 cerebrospinal fluid specimens were tested for pathogenic pathogens, and 80 of them were successfully detected. There was 1 case of human herpesvirus type I(HHV-I), 2 cases of varicella-zoster virus (VZV) and 77 cases of enterovirus(EV). The age group of 1~3 years′s EV detection rate was 66.67%, it is the highest, but overall, the EV detection rate, there was no significant difference among the four age groups (χ 2=3.147, P=0.369). The detection rate of EV in summer and autumn were 65.52% and 70.83%, respectively, which were significantly higher than those in spring and winter (33.33% and 0.00%), and there was a significant difference (χ 2=22.504, P=0.000). There was no significant difference in the positive rates of fever, headache and vomiting between EV-positive and non-EV-positive children ( P>0.05). There was no significant difference in the incidence of abnormal EEG and abnormal head MRI between EV-positive and non-EV-positive children ( P>0.05). Conclusions:In 2018, EV was the main pathogen of viral encephalitis and meningitis in children in Shijiazhuang city and surrounding areas, and EV detection rate was high in summer and autumn.

Fascaplysin is a natural marine product originating from sponges, attracting widespread attention due to its potential inhibitory activities against CDK4. However, its clinical application has been largely limited because of serious adverse effects caused by planar skeleton. To reduce the serious adverse effects, 18 tetrahydro-β-carboline analogs (compounds 6a-i and 7a-i) were designed and synthesized via breaking the planarity of fascaplysin, and the biological activities of the synthesized compounds were evaluated by MTT assay and CDK4/CycD3 enzyme inhibition assay. The title compounds showed varying degrees of inhibitory activities, especially the cytotoxicity of compound 6c against HeLa cells (IC 50 = 1.03 ± 0.19 μM) with quite weak cytotoxicity toward the normal cells WI-38 (IC 50 = 311.51 ± 56.06 μM), and the kinase inhibition test indicated that compound 6c was a potential CDK4 inhibitor. In order to further compare the action mechanisms of planar and nonplanar molecules on CDK4, the studied complexes of CDK4 bound with fascaplysin and three representative compounds (compound 6a-c) with bioactivities gradient were constructed by molecular docking and further verified through molecular dynamic simulation, which identified the key residues contributing to the ligands’ binding. By comparing the binding modes of the constructed systems, it could be found that the residues contributing significantly to compound 6c′s binding were highly consistent with those contributing significantly to fascaplysin’s binding. Through the design, synthesis of the nonplanar fascaplysin derivatives, and binding mechanism analysis, some valuable hints for the discovery of antitumor drug candidates could be provided.

目的 观察乌金液抑菌凝胶阴道外用治疗人乳头瘤病毒(HPV)阳性宫颈糜烂的效果.方法 将2018年7月—2020年2月在石家庄市第四医院和张家口市第一医院就诊的102例HPV阳性宫颈糜烂患者随机分为研究组和对照组各51例,研究组采用乌金液抑菌凝胶阴道注入治疗,对照组采用妇炎康栓阴道塞入治疗,均每晚1次,连续治疗3个月.观察2组治疗1个月及3个月后HPV感染程度评分(上皮组织特殊染色法评分)、HPV16阳性率、HPV18阳性率和治疗3个月后中医症状变化.结果 治疗1个月、3个月后,2组HPV感染程度评分均明显降低(P均<0.05),且研究组HPV感染程度评分和HPV16阳性率、HPV18阳性率均明显低于同期对照组(P均<0.05).研究组治疗3个月后外阴瘙痒、舌苔黄腻、脉弦数消失率和烦躁易怒改善率均明显高于对照组(P均<0.05).结论 乌金液抑菌凝胶治疗HPV阳性宫颈糜烂可有效抑制HPV感染,减轻患者症状.

目的 通过基于量化评估策略的护理干预在小儿支气管肺炎中的应用,探讨量化指标指导护理干预对患儿依从性和应激反应的影响效果,评估干预对预后的影响.方法 选择2017年2月至2020年9月收治的小儿支气管肺炎患者122例按随机数字表法分为对照组和观察组,每组61例.对照组采用常规护理,观察组在对照组基础上联合基于量化评估策略的护理干预,护理14 d后对患者效果进行评估,比较2组家属健康知识评分、应激反应、依从性及病情控制.结果 观察组疾病知识、饮食禁忌、急需就医知识、雾化吸入方式及定期复查知识评分高于对照组(P<0.05);观察组丙二醛(MDA)、隐性氧化蛋白产物(AOPP)水平均低于对照组(P<0.05);过氧化物酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及总抗氧化力(TAOP)水平高于对照组(P<0.05);观察组丙二醛患儿用药、运动及饮食依从性和患儿家属治疗方法、护理内容、服务态度满意度均高于对照组(P<0.05);观察组患儿治疗14 d后痊愈率100％,对照组痊愈率88.52％,观察组优于对照组(P<0.05).结论 基于量化评估策略的护理干预用于小儿支气管肺炎患者中能提高家属健康知识评估,降低患者治疗应激反应,能提高治疗依从性及患儿家属满意度,有利于改善预后.

目的:建立一种同时测定多柔比星、表柔比星及环磷酰胺血药浓度的方法并考察其在乳腺癌患者血浆中的浓度差异.方法:采用双波长高效液相色谱法,色谱柱为Waters XBridge C18(150 mm×4.6 mm,3.5μm),流动相为甲醇-0.01 mol· L-1磷酸二氢铵溶液-冰醋酸,梯度洗脱,流速1.0 mL· min-1,检测波长254nm(多柔比星、表柔比星)、190 nm(环磷酰胺).选取27例多柔比星、表柔比星联合环磷酰胺治疗方案的乳腺癌患者,检测其血药浓度.结果:多柔比星、表柔比星及环磷酰胺保留时间分别为8.86、9.89及13.95min,血药浓度分别在0.5～50 ng·mL-1、0.5～50 ng·mL-1及0.1～100μg·mL-1范围内呈线性且关系良好,方法回收率均在85.0％以上;日内、日间RSD均＜15％,不同剂量多柔比星/表柔比星与同剂量环磷酰胺联合使用,结果发现多柔比星高剂量给药方案组对患者环磷酰胺血药浓度有升高作用,但无统计学差异,表柔比星无此作用.结论:本研究建立了同时检测人血浆中多柔比星、表柔比星及环磷酰胺的方法,并且该方法适用于临床样本的测定,且通过不同剂量多柔比星、表柔比星与环磷酰胺联用,结果发现多柔比星对环磷酰胺血药浓度具有升高作用,但仍需进一步扩大样本量及开展作用机制研究.

Objective To investigate the expression of SDF-1 and its receptor CXCR4 in villi and decidua of patients with gestational hypertension,and to analyze the correlation between its expression levels and the pathogenesis of gestational hypertension.Methods The expression levels and extent of SDF-1,CXCR4 in villi and decidua of patients with gestational hypertension were detected by immunohistochemistry.Results With the deterioration and development of gestational hypertension,the expression levels and extent of SDF-1 and CXCR4 in placenta were gradually decreased(P<0.05).The expression levels of SDF-1 in villi in hypertension group and mild pre-eclampsia group were higher than those in decidua in both groups,however,the expression levels of SDF-1 in villi were lower than those in deciduas in severe pre-eclampsia group(P<0.05).The expression levels and extent of CXCR4 in villis were lower than those in deciduas in all the groups(P<0.05).Conclusion The changes of the expression levels and extent of SDF-1/CXCR4 in villi and decidua play an important role in the pathogenesis of gestational hypertension.

目的研究趋化因子基质细胞衍生因子-1（SDF-1）及其受体CXCR4在妊娠期高血压疾病患者绒毛、蜕膜中的表达,分析其表达水平变化与妊娠期高血压疾病发病之间的关系。方法采用免疫组织化学法检测妊娠期高血压疾病患者绒毛、蜕膜中SDF-1、CXCR4的表达强度及表达范围。结果随着妊娠期高血压疾病病情程度的加重,胎盘组织中SDF-1及CXCR4表达强度及范围均呈渐进性下降趋势（P〈0.05）。妊娠期高血压组、轻度子痫前期组SDF-1的表达绒毛强于蜕膜,重度子痫前期组SDF-1的表达绒毛弱于蜕膜（P〈0.05）。各组CXCR4的表达绒毛均弱于蜕膜（P〈0.05）。结论绒毛、蜕膜中SDF-1、CXCR4表达强度及范围的变化与妊娠期高血压疾病的发病有重要关系。

目的:研究胎盘中趋化因子SDF-1/CXCR4表达与妊娠期高血压疾病及新生儿出生体重之间的关系。方法:以10例妊娠期高血压患者为高血压组,10例轻度子痫前期患者为轻度子痫前期组,12例重度子痫前期患者为重度子痫前期组,35例正常产妇为对照组,以采用免疫组织化学法检测胎盘中SDF-1/CXCR4的表达强度,记录新生儿出生体重。结果:随着妊娠期高血压疾病病情加重,胎盘中SDF-1/CXCR4表达强度呈渐进性下降趋势,均低于对照组。对照组胎盘中SDF-1/CXCR4的表达与新生儿出生体重无统计学相关性。妊娠期高血压疾病患者胎盘中SDF-1、CXCR4的表达与新生儿出生体重有显著相关性。结论:胎盘中SDF-1、CXCR4表达强度变化与妊娠期高血压疾病发病及新生儿出生体重有重要关系。

目的观察孕期夫妻共同参加体操并配合盆底功能锻炼促进产后康复的效果。方法将197例健康初产妇随机分为2组。研究组95例由专人负责进行孕期夫妻体操和盆底功能锻炼的指导。对照组102例常规参加孕妇学校盆底功能健康宣教。结果产后42 d、3个月随访比较2组产后康复的优良率有显著性差异(P均<0.05)。结论孕期夫妻共同参加体操并配合盆底功能锻炼可促进女性产后盆底功能的康复,降低女性盆底功能障碍性疾病的发生率,并且对于提高生活质量和身心健康是积极有效的。

<正>女性性功能障碍(FSD)是指女性个体不能参与其所期望的性行为,且在性行为过程中不能得到或难于得到满足,这些感受包括性欲减退、性唤起障碍、性交痛、阴道痉挛。女性产后性功能障碍发病率为49%～83%,初产妇产后性问题发生率高达70.6%。其中性交疼痛发生率占第一位[1]。

目的 观察曼月乐避孕环对治疗乳痛症的辅助影响程度.方法 乳痛症患者100例作为对照组,同时患有乳痛症并且已经带上曼月乐避孕环的100例作为试验组,2组同时服用三苯氧胺治疗乳痛症,比较2组的治疗效果.结果 试验组疼痛改善较对照组明显,差异有统计学意义(P＜0.05).结论 曼月乐避孕环对缓解乳痛症有明显辅助治疗作用,值得推广.

Objective: To investigate the expression of SDF-1 and its receptor CXCR4 in placenta of patients of hypertensive disorder complicating pregnancy,analyze the relationship between their expression and the development of hypertensive disorder complicating pregnancy.Methods: The expression intension and extent of SDF-1,CXCR4 in placenta of patients of hypertensive disorder complicating pregnancy were measured by Immunohistochemical method.Results: Along with the deterioration and development of hypertensive disorder complicating pregnancy,the expression intension and extent of SDF-1 and CXCR4 in placenta were gradually decreasing.The expression intension and extent of SDF-1 were superior to the expression intension and extent of CXCR4.Conclusions: The variety of the expression intension and extent of SDF-1,CXCR4 has an important relationship with the development of hypertensive disorder complicating pregnancy.

<正>近年来,妊娠期营养及运动越来越受到学者的关注,孕期适量运动可以促进血液循环,提高血液中氧的含量,胎儿能通过脐带吸取更多的氧气和营养,更有利于胎儿的发育和智力开发,同时能消除孕妇身体的疲劳和不适,保持精神振奋和心情