Aimed as the heavy chain gene SLA-2 and the light chain gene β2m from Topics pigs,the complex was reconstructed with splicing overlap extension PCR(SOE,PCR) followed by cloning the complex gene into the expressing vector p2X and transforming the recombinant vector into E.coli.TB1 to be induced to express.The result indicated that the reconstructed complex gene was successfully expressed as a fusion protein MBP-SLA-2-(G4S)3-β2m with 84.1 ku in vitro.This research will pave the way to use the TOPICS swine SLA-2 and β2m complex to bind peptides in vitro.

By SOE PCR,the SLA-2 gene derived from SLA-Ⅰ heavy-chain molecule along with the light-chain gene β2m were linked to be a combined gene that named as SLA-2-(G4S)3-β2m,via a linker(G4S)3 encoding glycine/serine rich sequence.Then the combined gene SLA-2-(G4S)3-β2m was cloned into the expressing vector p2X followed by transforming the recombinant vector into E.coli TB1 and inducing them to be expressed.The result indicated that the combined gene SLA-2-(G4S)3-β2m was obtained by SOE PCR and cloned into p2X successfully.After being transformed into TB1 and induction,the combined gene was expressed to be a fusion protein MBP-SLA-2-(G4S)3-β2m with 84.1 ku.This research will supply a reference to recombine and express some relative genes in vitro.