Objective:To investigate the effect of LINC00261 on the radiosensitivity of nasopharyngeal carcinoma and tumor formation and its underlying mechanism in nude mice.Methods:qRT-PCR was used to detect the relative expression levels of miR-620 and LINC00261 in radiosensitive and radioresistant nasopharyngeal carcinoma tissues. After the 6-10B and HNE-3 cells were irradiated with 0, 2, 4, 6, and 8 Gy 60Coγ-ray, the relative expression levels of miR-620 and LINC00261 were measured by qRT-PCR. After over-expression or silencing of LINC00261 and inhibition of miR-620 expression, the cells were irradiated with 4 Gy 60Coγ-ray. Clone formation assay was performed to detect the radiosensitivity of nasopharyngeal carcinoma cells. Flow cytometry was used to detect cell apoptosis. Western blot was utilized to detect the expression levels of Cleaved caspase-3 and Cleaved caspase-9 proteins. Luciferase reporter assay was adopted to analyze the targeting relationship between LINC00261 and miR-620. The changes in tumor formation were observed in tumor-bearing nude mice. Results:Compared with the radiosensitive tissues, the expression of LINC00261 was significantly down-regulated, whereas that of miR-620 was significantly up-regulated in radioresistant tissues (both P<0.05). After different doses of irradiation, the expression of LINC00261 was significantly down-regulated, whereas that of miR-620 was significantly up-regulated in 6-10B and HNE-3 cells (both P<0.05). After overexpression of LINC00261 and interference with miR-620 expression, the expression levels of Cleaved caspase-3 and Cleaved caspase-9 were significantly up-regulated (both P<0.05), the cell apoptosis rate was remarkably increased ( P<0.05) and the cell survival fraction was significantly enhanced in 6-10B and HNE-3 cells ( P<0.05). LINC00261 targetedly regulated the expression of miR-620. Overexpression of miR-620 could attenuate the radiosensitization and pro-apoptotic effects of LINC00261 overexpression on nasopharyngeal carcinoma cells. LINC00261 overexpression could significantly reduce the tumor formation weight of nasopharyngeal carcinoma in nude mice ( P<0.05). Conclusion:Overexpression of LINC00261 can increase the radiosensitivity of nasopharyngeal carcinoma cells probably by targeted regulation of miR-620 expression.
目的 ：总结儿童甲状腺肿瘤围手术期护理经验，探讨儿童甲状腺肿瘤围手术期临床护理方法。方法 ：通过回顾性分析我科自 2016 年 5 月至 2019 年 5 月收治的50 例儿童甲状腺肿瘤患者的临床资料，总结甲状腺肿瘤患儿围手术期的护理方法及注意事项。结果 ：入组甲状腺肿瘤患儿共 50 例，男性患儿 16 例，女性患儿 34 例。术后病理回报甲状腺乳头状癌 49 例，甲状腺腺瘤 1 例。术后所有患儿伤口均一期愈合术后临时性声音嘶哑患儿 17 例，经雾化吸入及静脉使用激素后痊愈；短暂性手足麻木 7 例，经静脉补钙或口服钙剂治疗后症状缓解。结论 ：对儿童甲状腺手术及并发症的充分认识是做好围手术期护理工作的关键 ；做好围手术期护理，能有效地预防和减少并发症的发生，可提高甲状腺手术的疗效，缩短手术康复时间。
