目的 观察通心络胶囊对血管舒张功能及动脉粥样硬化的影响.方法 将240只新西兰兔随机分为正常组、模型组、阿托伐他汀组和通心络高、中、低剂量组,每组40只.正常组给予普通饲料喂养,其余各组均给予高脂饲料喂养建立高脂血症模型,在造模同时阿托伐他汀组给予阿托伐他汀溶液2.5 mg/kg灌胃,通心络高、中、低剂量组分别给予通心络胶囊溶液0.6、0.3、0.15 g/kg灌胃,灌胃体积均为3 ml/kg,每日1次.分别于实验后第3天、1周、2周、4周、6周检测各组血脂水平、主动脉管壁微血管密度及主动脉内皮依赖性舒张功能变化,并对血管舒张功能与管壁微血管密度进行相关性分析.结果 通心络高、中剂量组和阿托伐他汀组均能有效降低总胆固醇、甘油三酯和低密度脂蛋白水平(P ＜0.05或P＜0.01);与模型组比较,阿托伐他汀组和通心络高剂量组在给药4周、6周时主动脉舒张率明显升高,管壁微血管密度降低(P ＜0.05或P＜0.01),通心络中剂量组在给药6周时主动脉舒张率也升高,管壁微血管密度降低(P＜0.05);内皮依赖性血管舒张功能与管壁微血管密度呈负相关(r=-0.824,P＜0.01). 结论 通心络胶囊通过降低血脂水平、抑制动脉管壁微血管的滋生,来改善血管舒张功能,从而延缓动脉粥样硬化的进一步发展.

Objective To observe the protective effects ot Jiweiling lyophilized powder(JWLF) on the neuron of spinal cord injury(SCI) rats,and the influence on primary neuron apoptosis induced by glutamate in vitro,also explore the molecular mechanism of JWLF on the motor neuron disease(MND).Methods The SCI model of rats were developed with static compressive pressure of 30 g on the spinal epidural for 10 min,the neuron function was evaluated at 1 d,7 d and 14 d after the surgery and the morphologic changes of the damaged spinal were determined.The primary cortical neuron was damaged by glutamate at low concentration,then measure the viability and the expression of Bcl-2,Bax with methods of MTT,flow cytometry and Western blot.Results(1) JWLF could obviously improve BBB rating score on SCI rats at different time and release the edema of spinal neurons.(2) JWLF could enhance neuron activity in rat which suffered from glutamate and alleviate neuronal injury.(3) JWLF remarkably inhibited the neuron apoptosis induced by glutamate.(4) JWLF could balance the expression of Bcl-2 and Bax of neuron.Conclusion JWLF could promote hind limb motor function of SCI rats through enhancing the neuron activity and restraining the neuron apoptosis.

Objective To explore the interventional effects of Tongxinluo super-micropowder on infarction area and neurological behavior of focal cerebral ischemia.Methods Using craniotomy ligation a side of the middle cerebral artery established the model of focal cerebral ischemia(MCAO),after filtration male SD rats were randomly divided into sham-operation group,model control group,TXL-H,TXL-M,TXL-L and nimodipine.After 3 d、7d、14 d,the neurological outcomes were scored.The infarct size of brain was measured by TTC staining.Results After 3d,compared with the model control group,the score of neurological symptom and the infarct size of brain were statistically significant in TXL-H and nimodipine(P<0.05).TXL-H and nimodipine were not statistically significant (P>0.05).TXL-M,TXL-L and model control group were not statistically significant(P>0.05 );After 7d,compared with the model control group,TXL-H,TXL-M,nimodipine were statistically significant(P<0.05,P<0.01).TXL-H was better than TXL-L.After 14d,compared with the model control group,TXL-H,TXL-M,TXL-L, nimodipine were statistically significant(P<0.05,P<0.01).TXL-H was better than either of TXL-M,TXL-L or nimodipine(P<0.05).Conclusion Tongxinluo super-micropowder can significantly improve the rats of focal cerebral ischemia in neurological deficits and infarction area.Tongxinluo showed obvious protective effects on focal cerebral ischemia in rats,but the mechanism need further study.