Objective To study the clinical diagnosis value and the clinical significance of anti-mitochondrial antibody(AMA-M2),antinuclear antibody(ANA)and immunoglobulin(IgG,IgM,IgA)in patients with Primary Biliary Cirrhosis(PBC) and hepar lesion or autoimmune diseases.Methods The serum levels of AMA M2,ANA and IgG,IgM,IgA were separately determined by ELISA,Western blot and scattering immunoturbidimetric assay in 12 patients with PBC,27 ones with viral hepatitis,21 ones with AIH,33 ones with SLE,34 ones with RF,26 ones with SS and 20 cases of nomal controls.Results The positive rate of PBC group was significantly higher than that in other groups(P<0.01).In humoral immunity,the serum level of IgG in patients with PBC was significantly higher than ones with SLE and RF,but it was no statistical significance comparing with viral hepatitis and AIH.Comparing with other groups,the serum level of IgA in patients with PBC was no statistical significance but IgM was significantly higher.Conclusion The results indicate that the measurement of AMA M2 is highly specific and sensitive in diagnosis PBC.It is useful for differentiation of PBC and viral hepatitis,AIH by detecting AMA M2 and ANA,and also for differentiation of PBC and SLE,RF,SS by detecting levels of immunoglobulin.

To evaluate the correlation of T cell subsets,serum immunoglobulin and complement levels with different kinds of syndromes of ZhongYi of post hepatitis liver cirrhosis,84 patients have been diagnosed as four kinds of syndrome of Zhong Yi including 40 patients with syndrome of accumulated dampness-heat(group①),20 patients with syndrome of blockade of dampness due to qistagnation(group②),12 patients with syndrome of Yin deficiency of liver and kidney(group③),11 patients with syndrome of Yang deficiency of spleen and kidney(group④).T-lymphocyte subsets(by flowcytometry) and serum immunoglobulin levels were measured for all the patients.Compared with control group,the IgA、IgG levels were increased significantly in all group(P0.05,P0.01).CD8+ T cells and NK amounts were decreased(P0.05)in group①.CD8+T cells were decreased significantly(P0.01),and CD4+ T cells were decreased(P0.05)in group②.CD4+/CD8+ ratio was significantly increased in group ② than in any other groups.The different kinds of syndrome of ZhongYi were related to status of immunity,and the syndrome of ZhongYi changed with the status of immunity.CD4+,CD8+,CD4+/CD8+ ratio,immunoglobulin levels determinations may help to define syndrome of ZhongYi and used to clinical diagnosis and treatment.