交叉引物扩增技术是一种新型的恒温扩增技术,为转基因生物的检测提供了新的手段.本研究针对转基因水稻Bt63的3’端边界序列设计5条CPA引物进行恒温扩增,反应条件为62℃50min.结果表明该方法特异性好,检测限为0.1％,可用于转基因水稻Bt63品系特异性检测.

AbstractThe techniques used to detect genetically modified organisms (GMO), including qualitative polymerase chain reaction (PCR), quantitative PCR, enzyme-linked immunosorbent assay (ELISA) and many others, are systematically described and discussed. The application progress of GMO in species-specific detection, endogenous genes, standard substances and restraining factors influencing detection are reviewed. The ongoing problems and development prospects of detection techniques of GMO are also pointed out.