Objective To isolate the adenovirus from clinical sample taken from a patient with respiratory tract infection and to identify the type of the isolated adenovirus. Methods One adenovirus positive sample from hospitalized patients with respiratory tract infection was seeded onto HeLa cell. Adenovirus strain was isolated and purified from the virus plaque assay. The hypervarible region of hexon gene was amplified by the extracted viral DNA via polymerase chain reaction( PCR). The PCR product was sequenced and the sequence was subjected alignment and basic local alignment search tool( BLAST) searching in GenBank. Results BLAST search analysis on Hexon gene showed that the adenovirus strain isolated was identical to human adenovirus type 3 strain USA / ak34_AdV3a2 with 100% homogeneity. Conclusion The adenovirus isolated from a clinical sample is identified as human adenovirus type 3.

Objective To investigate the etiological and epidemiological features of respiratory viral infection in hospitalized patients from Harbin region as a reference for clinical treatment and prevention of viral infections.Methods The nasopharyngeal aspirates samples were taken from 521 hospitalized patients with respiratory tract infection from August 2009 to September 2010.Seven kind respiratory viruses were screened using the fluresenant antibodies with direct immunofluorescence assay(DFA).Results Among all the 521 samples,74(14.2%) were respiratory virus positive.And among the 74 viral positive samples,single virus infection was 39 and more than one kind virus complex infection was 79 which accumulated as 118 viral infection.Among the 118 viral infections,respiratory syncytial virus accounted for 37.3%(44),adenovirus 5.1%(6),influenza virus A 31.4%(37),influenza virus B 7.6%(9),parainfluenzaⅠ16.2%(12),parainfluenzaⅡ0.8%(1),parainfluenza Ⅲ7.6%(9).The viral infection differences in season distribution were significant,and the differences in the others(age,gender and disease) were not significant.Conclusion RSV is dominant in respiratory infection,followed by influenza virus A.