Objective To study the Inhibitory effects of 20(S)-ginsenoside Rg3loaded human albumin nanopaticles(SPG-Rg3-HAS-NP)on human cervical cancer Hela cells in vitro.Methods Hela cells were treated with normal saline、blank human albumin nanopaticles、pure 20(S)-ginsenoside Rg3(SPG-Rg3)and 20(S)-ginsenoside Rg3loaded human albumin nanopaticles(SPG-Rg3-HAS-NP)respectively.Hela cells growth inhibition rates were detected after 24h、 48h、72hand 96hbeing treated with different drug concentration by MTT assay.Results Blank human albumin nanopaticles had no cytotoxicity on Hela cells without concentration-dependence and time-dependence in vitro.SPG-Rg3 group SPG-Rg3-HAS-NP group had significant anti-proliferation effect on Hela cells growth by MTT assay,and Hela cells growth had respectively concentration-dependence and time-dependence to SPG-Rg3group SPG-Rg3-HAS-NP group.Hela cells growth seemed to be platform phase and even have a down curve tendency after concentration of 80 μg/ml SPG-Rg3in SPG-Rg3group,but Hela cells growth had a continue ascend curve in SPG-Rg3-HAS-NP group.The SPG-Rg3-HAS-NP group had higher inhibitory effect than SPG-Rg3group after concentration of 20μg/ml SPGRg3and after 72hadministration(P0.05).The above results indicated anti-proliferation effect on Hela cells growth in SPG-Rg3-HAS-NP group had a certain time last and the SPG-Rg3-HAS-NP had a certain sustained-releasing effect.Conclusion SPG-Rg3-HAS-NP has a significant anti-proliferation effect on human cervical cancer Hela cells with concentration-dependence and time-dependence in vitro.