Objective:To investigate the effect of human Leptin on the proliferation of human circulating T lymphocytes in vitro and the influence of Leptin on the action of PHA on the proliferation of human circulating T lymphocytes.Methods:PBMC were isolated from hepartinized venous blood of normal donors by density-gradient sedimentation over Ficoll-Hypaque and cultured in the 1640 complete medium.T lymphocytes were then obtained by depleting the monocyte and B cell populations.Cells were then cultured in the 1640 complete medium for 56 h in the presence of PHA.The proliferative effect was assessed by means of incorporation of thymidine.TdR was added and cultured for 16 hours.Cells were then processed in the harvester to measure the incorporation of [()~3H] thymidine.Radioactivity was determined by liquid scintillation counter.Results:The results showed that Leptin alone did not affect the proliferation of T lymphocytes,but it could enhance the effect of PHA on the proliferation of T lymphocytes at the concentrations of PHA from 2-8 μg/ml.A dose-response studies of T lymphocyte proliferation showed that the maximal effect of Leptin were observed at 10 nmol/L.Leptin did not affect the proliferation of T lymphocytes when the concentrations of PHA were over 8 μg/ml.Conclusion:The studies demonstrated that Leptin alone did not affect the proliferation of lymphocytes,but it could enhance the action of PHA on cell growth in a dose-dependent manner.

Objective To Discussion the Correlation between leptin and the proliferation of PBMC.Methods PBMC were isolated from hepartinized venous blood of normal donors by density-gradient sedimentation over Ficoll-Hypaque and cultured in the 1640 complete medium.Cells were than cultured in the 1640 complete medium for 56 h in the presence of leptin and PHA or ConA.The proliferative effect was assessed by means of incorporation of thymidine. TdR was added and cultured for 16 hours.Cells were then processed in the harvester to measure the incorporation of thymi-dine.Radioactivity was determined by liquid scintillation counter.Results The results suggested that leptin could affect the proliferation of PBMC and it could also enhance the effects of PHA or ConA on the proliferation of PBMC.Conclusion Leptin can affect the proliferation of PBMC.

Objective To investigate the effect of cyclophosphamide and hydrocortisone on T cell subpopula-tions in thymus, peripheral blood and spleen of mice. Methods Cyclophosphamide and hydrocortisone were injected into the mice respectively and the number of T cell subpopulations in thymus, peripheral blood and spleen of mice were determined by flow cytometry, using the method of direct immunofluorescence. Results Cyclophosphamide reduced strongly CD4+ , CD8+ thymocyte subpopulation and hydrocortisone administration induced a significant decrease in the percentage of blood CD4+ cells in peripheral blood and spleen. Conclusion Cyclophosphamide has impact on thymocyte subpopulation, hydrocortisone has impact on peripheral blood and spleen T lymphocytes.

目的:探讨基因芯片技术分析高低不同转移能力肺癌细胞系的差异表达基因.方法:采用基因芯片技术检测具有高低不同转移能力的肺癌细胞系BE-1和LH-7的差异表达基因.抽提细胞mRNA,利用荧光标记dUTP逆转录制备cDNA探针,与人肺癌表达谱基因芯片杂交,以ScanArray 4000荧光扫描仪扫描芯片上两种荧光信号,获得的荧光信号图像用计算机分析,每点上两种荧光信号的强度分别代表Cy3-dUTP和Cy5-dUTP的量,最后计算每点的Cy5和Cy3的比值.结果:在所检测的人高低肺癌转移细胞系BE-1和LH-7中,20个基因有表达差异,其中高表达14条,低表达6条.结论:基因芯片技术为筛选人类肺癌转移基因提供了有效方法.

本文对10例心性猝死的病人进行分析,以探讨其危险因素、先兆症状、诱因、预防保健措施.

目的观察CIK(cytokine induced killer)细胞对S180和H-22荷瘤鼠的抑制肿瘤作用.方法建立S180和H-22荷瘤鼠动物模型,静脉输入CIK细胞做抗肿瘤治疗,同时设LAK对照,用同位素法检测经治疗后荷瘤鼠T细胞增殖及NK细胞毒活性.结果CIK细胞对S180和H-22荷瘤鼠的抗肿瘤作用显著强于LAK细胞.结论CIK细胞对S180和H-22荷瘤鼠有明显的抗肿瘤作用,并且优于LAK细胞.

目的探讨HSV-1感染Hela细胞时,是否发生凋亡.方法用扫描电镜及透射电镜观察感染72h的Hela细胞的形态变化.结果正常的Hela细胞表面有丰富的微绒毛.细胞间隔清晰,可见幼稚连接,连接不紧密.胞浆内线粒体嵴结构完整,并可见大量的粗面内质网及游离核糖体,极少的脂滴.细胞核内多为常染色体,核仁清晰,核浆比约1:1.凋亡细胞中核内异染色质增多,染色质浓缩成块状,边集于核膜.线粒体轻度肿胀,溶酶体代偿性增多.核膜、胞膜、各细胞器膜均完整.坏死细胞的染色质呈不规则的团块状,但不象凋亡细胞那样集中于核周边.细胞膜、核膜和细胞器的结构破坏,线粒体空泡变,细胞崩解,胞浆及其内容物外泄.HSV-1感染的Hela细胞体积明显缩小,核浆比例明显增加.细胞表面的微绒毛断裂、减少,细胞膜球状突起.核膜完整,核致密,呈块状分布,边集于核膜.细胞膜、细胞器结构完整.可见出泡现象和凋亡小体形成现象.在胞核内可见病毒体.结论 ①HSV-1感染He1a细胞时,造成细胞死亡形式是核死亡形式即凋亡;②HSV-1感染Hela细胞时,细胞凋亡占绝对优势,同时伴有细胞坏死.