[Objective] Analyzing metabolic profiling changes in urine of type 2 diabetic rat model based on gas chromatography-mass spectrometry tech nology(GC-MS),to explore the metabonomics character and micromolecule marking compound closely related to type 2 diabetes.And proviede a new idea for the research of pathogenesis of type 2 diabetes.[Method] Sprague-Dawley rats were fed with high-fat diet for 9 weeks and on the fourth week end injected with streptozotocin(STZ) in a dose of 30 mg.kg-1 to set up type 2 diabetic rat model.Put into the mold after fed with high fat diet for 4 weeks.Fasting blood glucose(FBG) was monitored weekly after STZ injection.Urines of normal rats and model rats were collected at first to ninth week after STZ injection.Triglyceride(TG),total cholesterol(CHO) and plasma insulin(FINS) were measured at 9th week after STZ injection and the rats were sacrificed.The metabolic profiles were analyzed using trimethylsilane(TMCS) derivatization and gas chromatography-mass spectrometry(GC-MS).[Results] Compared with the control rats,the FBG,TG and CHO were significantly higher while the FINS was reduced in the model rats.The difference of metabolic profile of the normal group and model group was more and more outstangding as the time went on.Comparative study was applied and fourteen biological markers were different between the normal and model rats.[Conclusion] Metabonomics method with GC-MS was suitable for urinary metabonomics study of type 2 diabete model animals,reflecting the trend of discreting and closing of urinary metabolic profile between the normal group and model group,as well as inter-normal group and inter-model group,it may reflect a certain extent to the pathological changes of type 2 diabetic rats.
Metabonomics is a new science developing under the background of vigorously developing systematic biology.It can monitor state of metabolic network,diagnose disease,evaluate therapeutic effect and predict trend of devlopment of human health though metabolic fingerprint as a whole.With the initiation and development of diabetes,the metabolite will necessarily alter and metabonomic technique provide panoramic information for the metabolic status of diabetes to offer a new way of thinking for its personalized diagnosis and treatment.This paper mainly reviews the recent application progress of metabonomics in diabetes research.
目的研究冠心苏合丸物质组释放动力学特征。方法以GC-MS法为检测手段,60%乙醇为释放介质,在(37±0.5)℃温度下,采用浆法100 r/min测定同一处方不同厂家生产的和同一厂家生产的不同批号的冠心苏合丸的物质组释放度,以考察不同厂家、不同批号冠心苏合丸物质组释放动力学特征的差异。结果 A厂(批号9012742)、A厂(批号9011245)、A厂(批号8013353)、B厂、C厂和D厂的冠心苏合丸在240 min内物质组的平均累积释放率分别是:104.12%、107.38%、93.79%、69.33%、75.30%和101.36%,释放曲线的Weibull分布参数T50分别为(184.38±19.03)、(186.51±8.65)、(193.38±4.87)、(186.90±10.71)、(246.32±12.90)和(114.23±5.89)min,Td分别为(199.42±22.47)、(202.41±9.16)、(220.64±8.34)、(300.06±34.43)、(374.04±40.57)和(168.07±10.79)min。结论不同厂家的冠心苏合丸物质组释放动力学特征存在显著性差异,同一厂家不同批号间的冠心苏合丸物质组释放动力学特征无显著性差异。
