In this paper,the protein removing methods and their effect factors on polysaccharide extraction from Porphyra yezoensis were studied.Sevag method and TCA method were used to remove protein from Porphyra polysaccharide extraction respectively,and effects of different ratios of solvent volume,dosages,treating temperature and time and other main factors on protein removing were studied respectively.The results showed that the optimum conditions of protein removing with Sevag method were: 3∶1 ratio of chloroform:n-butanol,3∶1 ratio of sample: chloroform-n-butanol,30 min stirring time;and the optimum conditions of protein removing with TCA were: 80 ℃ temperature,30 min treating time,4% trichloroacetic acid.The comparison between Sevag method and TCA method indicated that protein removing efficieny with TCA method was higher than that with Sevag method.
Objective:In order to research some biological functions of crude polysaccharide from Porphyra yezoensis,the lymphocytes and the sertoli cells were deteced.Methods:After primary culturing,the spleen lymphocytes of mice and rats and the sertoli cells were separated into 96-well cell culture plates.Different concentrations of crude polysaccharide from P.yezoensis(0.05,0.5,5,10 mg/mL)were selected and added to the culture.The cell survival rates were assessed by MTT assay.Results:Obviously,the crude polysaccharide from P.yezoensis can enhance the proliferation of these cells.When the concentration of the polysaccharide is 10 mg/mL,the rate of the proliferation reached 137.3%,149% and 454.5% respectively.Conclusion:The results indicates that crude polysaccharide from P.yezoensis may improve the immunity and the procreative function.Especially,the effect on the proliferation of the sertoli cells has not been reported yet.
紫菜多糖是海藻类多糖中的一种,在结构上与肝素(hep)相似,都是多糖的硫酸酯类[1].周慧萍等[2-5]研究发现紫菜多糖在抗凝血、降血脂、提高免疫力、抗衰老等方面都有一定的生物活性.张伟云等[6]研究发现一种紫菜多糖能开发成为免疫抑制剂用于临床免疫亢进的治疗.Yoshizawa等[7]还发现条斑紫菜多糖具有激活巨噬细胞的功能,可提高肿瘤坏死因子(TNF)的产量.以上研究说明紫菜多糖可以开发为一种重要海洋药物.目前关于紫菜多糖提取的影响因子还缺乏系统的研究,因此本文主要对影响紫菜多糖粗提的重要外界因素进行研究,为今后紫菜多糖提取工艺和紫菜多糖药物研究提供重要参考依据.
