Objective To study the role of TLR4 in angiotension II(Ang II )-induced cardiac fibrosis in hypertensive mice.Methods Eighteen male wild-type mice were divided into blank control group,Ang II group,and TLR4 blocking group(6 in each group).A hypertension model was established by Ang II perfusion with a micropump.Blood pressure was measured in mice through the tail artey cuff and heart function of the mice was tested by echocardiacgraphy after injection of TLR4 neutralizing antibodies through the tail artery.Cardiac fibrosis in the mice was observed with immunohistochemical and Masson staining.Expressions of myocardial interleukin 1(3 and mononuclear cell chemotactic protein 1 mRNA were detected by RT-PCR.Results The blood pressure was lower,the left ventricular wall was thinner,the inner diameter of left ventricule at the end-diastolic phase was larger,the number of infiltated Mac-2 positive macrphygocytes was less,the expression level of interleukin 1βand mononuclear cell chemotactic protein 1 mRNA was lower,and the fibrosis of myocardial interstitium and peripheral blood vessels was milder in TLR4 blocking group than in Ang U group(P<0.05).Conclusion TLR4 participates in Ang II -induced cardiac fibrosis in hypertensive mice by mediating inflammatory reactions.

目的 研究ICP22蛋白缺失是否影响病毒的感染复制以及ICP22蛋白在病毒水平上功能.方法 利用同源重组方法用绿色荧光蛋白(green fluorescent protein,GFP)的编码基因取代ICP22蛋白的编码基因,以流式细胞仪分选结合蚀斑筛选的方法得到ICP22蛋白缺失的单克隆重组病毒.结果 对ICP22蛋白缺失重组病毒进行绿色荧光的表达情况的镜下观察、GFP基因序列的测定、重组病毒中GFP基因的定量PCR检测、GFP蛋白表达的Western 印迹 检测等方法进行了验证,成功构建了ICP22蛋白缺失的重组病毒,并在研究过程中发现ICP22蛋白缺失的重组病毒出现感染复制停滞的现象.结论 ICP22蛋白缺失的重组病毒出现感染复制停滞的现象提示其在细胞中具有重要的功能.

Enterovirus 71 (EV71) is a major etiological agent of hand-foot-and-mouth disease (HFMD). Since no effeetive antiviral agents are available,developing effective EV71 vaccines for prevention of HFMD has been the control measure of choice. Currently, the research of EV71 vaccines has made certain pro-gress,and various EV71 vaccines, including attenuated vccines, inactivated vaccine, VP1 subunit vaccine and DNA vaccine, are being developed. Although various EV71 vaccines have certain efficacy, the optimal vaccine needs to be identified by evaluating the safety and protective efficacy of various EV71 vaccines.

Objective To study the combined effect of foreign retinoblastoma 94 gene and ionizing radiation on the growth of esophageal carcinoma cells K150. Method K150 cells was transfected by recombinant Rb94 gene adenovirus vector and irradiated by 6 Gy ~(l37)Cs γ-ray after transfection. The cohorts were divided into blank control, Ad-lacZ, Ad-Rb94, irradiation and Ad-Rb94 combined with irradiation. Cell growth, cell cycle, cell apoptosis and expression of retinoblastoma 94 gene of K150 cells were detected. Results The growth of K150 cells transfected with Ad-Rb94, radiation and Ad-Rb94 combined with irradiation group at 7 day after infection was all inhibited. The group of Ad-Rb94 combined with irradiation resulted in greater inhibition of cells growth, reached 45.49 % , compared with Ad- Rb94 infection (20.26 % ) and radiation group (35.11 % ) (χ~2 = 14.25,P <0.05). Cells of G_1 phases and apoptosis of K150 cells for the Ad-Rb94 combined with irradiation group were the highest, reached 73.4% and 15.7% , respectively. The combination of Ad-Rb94 and irradiation resulted in the greatest expression of retinoblastoma 94 gene, which was 1.87 fold and 2.92 fold of Ad-Rb94 infection and irradiation, respectively. Conclusions Ad-Rb94 gene transfection combined with irradiation shows the synergism for the inhibition of esophageal cancer K150 cells growth.