本研究通过对比不同中空纤维超滤膜孔径、洗滤液及剪切速率,建立猪肺炎支原体中空纤维浓缩纯化工艺;并采用该工艺对猪肺炎支原体活菌液和灭活菌液进行浓缩纯化,测定回收率、杂蛋白去除率及纯化前后抗原免疫原性变化.结果表明:750kD和0.1μm的中空纤维膜柱纯化效果较好,杂蛋白去除率达80％以上;不含猪血清的Friis基础液洗滤,抗原回收率高于PBS洗滤;浓缩纯化过程中剪切速率可控制在2000～6000s-1.猪肺炎支原体活菌液经0.1μm中空纤维膜浓缩纯化抗原回收率达100％;灭活抗原纯化前后按相同含量配制疫苗免疫兔子,猪肺炎支原体抗体效价没有明显差别,说明纯化后免疫原性未损失.

<正>新生儿脐带残端是一个开放的伤口,又有丰富的血液,是病原菌生长的好地方,如处理不当,病菌就会趁机而入,引发新生儿脐炎,处理不及时,甚至会导致发生新生儿败血症。目前临床上常用新生儿脐带消毒仍为碘酊,尤其是基层医院。但新生儿皮

Objective To compare the germicidal efficacy of three hydrogen peroxide compound disinfectants.Methods Suspension quantitative germicidal test and field test were used to observe the germicidal efficacy and stability of three hydrogen peroxide compound disinfectants.Results The average killing logarithm values of Escherichia coli,Staphylococcus aureus and Candida albicans exposed to hydrogen peroxide glutaraldehyde compound disinfectant for 20 min were 3.12,3.02,2.08 respectively;exposed to hydrogen peroxide benzalkonium bromide compound disinfectant for 20 min were 6.98,6.20,5.03 respectively;exposed to hydrogen peroxide polyhexamethylene biguanidine compound disinfectant for 20 min were 6.16、5.96、5.08 respectively and exposed to 30 g/L hydrogen peroxide disinfectant for 30 min were 2.99,2.62,1.65 respectively.Conclusion The three kinds of compound disinfectants have better germicidal efficacy than hydrogen peroxide disinfectant alone.The hydrogen peroxide polyhexamethylene biguanidine compound disinfectant has the best germicidal efficacy.