AbstractIn der vorliegenden Arbeit wird die Block‐Herstellung von Desoxy‐ribo‐oligonucleotiden nach der Phosphortriester‐Methode ohne kondensierende Agentien untersucht.

AbstractEin neuer Typ eines polymeren Trägers für die Oligonucleotid‐Synthese entsteht durch auf eine inerte Polytetrafluorethylen‐Matrix aufgepfropftes Polystyrol.

The work of the authors are discussed, that they have been engaged in since the late forties, on synthesis and investigation of mono- and oligonucleotide derivatives. One class of mixed biopolymers (mono (oligo) nucleotides) is used to demonstrate the effect of covalent and noncovalent intramolecular interactions on the character of various protein-nucleic acid contacts. General approaches to creating regioselective reagents are outlined, that can be widely used for modification of both proteins and nucleic acids. Designing and application of some of such reagents is analyzed.

Nucleotidyl-(5' leads to N)-amino acids containing different heterocycle bases: adenine, guanine, hypoxanthine, cytosine, uracyl, and aromatic amino acids: phenylalanine, tyrosine and tryptophan, have been investigated by proton magnetic resonance and circular dichroism. For all the compounds studied folded conformation have been shown stabilized by hydrophobic interaction in aqueous solution. The comparison of the results of the studied nucleotidyl-(5' leads to N)-amino acids unable us to build four secondary structure types in these very compounds. Phenylalanine and tyrosine derivatives of purine nucleotides can be regarded as the first type, tryptophan derivatives of purine nucleotides as the second type, phenylalanine and tyrosine derivatives of pyrimidine nucleotides as the third type and tryptophan derivatives of pyrimidine nucleotides as the fourth type. For each group of these compounds conformational models have been built. In all these compounds the anti-conformation has been proved to exist.

AbstractEin Copolymerisat aus Styrol, Divinylbenzol und p‐Methoxy‐p′‐vinyltriphenylcarbinol nach Zh.

AbstractEin durch Monomethoxy‐triphenylmethylchlorid (Tr) modifiziertes Copolymerisat aus Styrol und Divinylbenzol (Ps) wird als polymerer Träger an das Desoxynucleosid (I) gebunden.

AbstractBei der Verwendung von polymeren Trägern bei der Herstellung von Oligo‐nucleotiden sind die Ausbeuten, vermutlich infolge der starken Quellung der Träger, gering.

AbstractBei der Phosphorylierung von Nucleosiden ist es schwer, das Phosphorylierungsprodukt mit guter Ausbeute von den Ausgangs‐ und Nebenprodukten zu trennen.