Arabidopsis Tóxicos en Levadura (ATL) proteins are members of the RING-H2-type E3 ubiquitin ligase subfamily and play important roles in plant physiological processes and stress responses. However, their roles in eggplant (Solanum melongena) remain largely unexplored. In this study, 145 putative SmATL family members were identified and systematically analyzed. Phylogenetic analysis classified them into eight subgroups, and chromosomal mapping revealed their uneven distribution across the eggplant genome. Collinearity analysis identified 37 pairs of collinear genes, while gene structure and motif analyses revealed structural diversity and conserved protein motifs among SmATL members. Under cold stress, transcriptomic analysis showed that several SmATL genes were upregulated, with SmATL59 showing a strong response. qRT-PCR confirmed the gradual induction of SmATL59 during cold treatment. Subcellular localization showed that SmATL59-GFP was mainly localized to the plasma membrane. Overexpression of SmATL59 in Nicotiana benthamiana enhanced cold tolerance, reduced ROS accumulation, increased antioxidant enzyme activities, and upregulated cold-responsive genes. Yeast two-hybrid and luciferase complementation imaging assays demonstrated that SmATL59 interacts with SmATG5a. These results highlight the potential role of SmATL59 in improving cold stress tolerance.

Since type 2 diabetic patients often develop resistance to metformin as the progresses of diabetes, and almost all type 1 diabetic patients need receive insulin injection for hyperglycemia control. It is important to explore novel strategies with different mechanisms for diabetes management. Glucose-induced osmotic diuresis, known as polyuria, is the first clinical symptom in severe type 2 diabetes mellitus (T2DM) and type 1 diabetes mellitus (T1DM). Drinking green tea or black tea effectively mitigates diabetic symptoms including polyuria, polydipsia, polyphagia and hyperglycemia in db/db mice via regulating renal aquaporin 2 and urine transporter A1 (UT-A1), in favor renal water reabsorption. This unique mechanism of action of tea could be useful for the treatment of diabetes in humans. In this study, we found that drinking Large-leaf yellow tea (LYT) for 5 weeks effectively ameliorated polyuria, polydipsia, polyphagia, hyperglycemia and excessive body weight gain, as well as upregulated renal water reabsorption associated proteins, including protein kinase C-alpha (PKC-α), membrane PKC-α and glycosylated UT-A1 in db/db mice. Four-days experiment were also confirmed the rapidly response of these proteins in favor renal water reabsorption and the amelioration of diabetic symptoms by LYT. We also found that green tea drinking effectively mitigated symptoms of diabetes in a mouse model for T1DM via upregulating these proteins. Moreover, green tea drinking prevented hepatorenal damage caused by hyperglycemia as suggested by the reduced levels of aspartate aminotransferase and creatinine in serum and the enhanced antioxidant defense system in liver and kidney. These results suggest the possible application of tea or tea constitutes in the clinical treatment of severe T2DM and T1DM, and the kidney is the target organ.

BACKGROUND: Tea plants (Camellia sinensis) are highly sensitive to cold stress and exhibit significant genetic variability in cold tolerance. Although multiple studies have explored low-temperature response mechanisms, the physiological and proteomic basis underlying variation in cold tolerance among Camellia sinensis cultivars remain unclear. In this study, we conducted a comparative physiological and proteomic analysis of a cold-tolerant (SCZ) and a cold-sensitive (YH9) variety under cold acclimation and freezing stress. RESULTS: The cold-tolerant genotype SCZ exhibited greater cold tolerance than YH9, as evidenced by the accumulation of starch granules, less pronounced structural changes in chloroplasts, and a relatively higher Fv/Fm ratio. Using an iTRAQ-based proteomic approach, a total of 201, 261, 71, 70, 153 and 148 differentially abundant proteins (DAPs) were found in the comparisons of NA-CS vs. NA, CA vs. NA, and CA-CS vs. CA in SCZ and YH9, respectively. During cold acclimation, SCZ reduced light-induced damage through downregulation of photosynthesis-related proteins (Lhca, Lhcb, Psb, Psa, and ATPF) and ROS metabolic proteins (APX-2, GST-2/3/4/5/6, GRX-2), while upregulating the anthocyanin-related protein BZ1. In contrast, YH9 showed uncoordinated expression of ROS-scavenging enzymes, such as downregulation of GST-5 and PRX-2, alongside upregulation of SOD-2, GSR-1/2, GST-1, and GST-8. Notably, under freezing stress, cold-acclimated SCZ seedlings upregulated photosynthesis-related proteins (Lhca2, Lhcb2, PsbQa, PsaH, PsaN) and polyphenol metabolic pathway-related proteins (FLSc, ALDH), while downregulating ROS metabolism-related proteins (SOD-1, CAT-1/2/3, PRX-2). This response differred from that of non-acclimated SCZ seedlings, as well as both non-acclimated and acclimated YH9 seedlings under short-term freezing stress. Protein-protein interaction network analysis identified PSAN, LHCB5, PSAF, LHCB4.1, and others as potential hub proteins, suggesting their central regulatory roles in cold tolerance. However, their biological functions require further validation. CONCLUSIONS: Our findings reveal distinct proteomic and physiological strategies between two tea cultivars with contrasting cold tolerance, providing candidate targets and a theoretical foundation for future breeding efforts.

Micrografting technology has gained popularity in model plants, with the advantages of a wide grafting range and small space. However, this technique has not been fully explored in tea plants. In our study, different rootstocks [radicle (obtained from the germination in seed), epicotyl without cotyledons, epicotyl with cotyledons, tea varieties] and scion (red branch, green branch) grafting combinations were used to estimate the survival rate, plant growth, the compatibility behavior, and cold tolerance of grafted seedlings. Our results showed that the higher survival rate and shooting rate were observed in radicle (obtained from the germinated seed diameter ≥ 15 mm, D3) as the rootstock. Also, the same growth indicators were found in the green branch as scion and radicle as rootstock (GB\R) were higher than that of other grafting combinations. In addition, the grafted seedlings of LJ43 as rootstock had the best growth rate, and the vascular bundle bridge was completely established in SCZ as scion and LJ43 as rootstock (SCZ/LJ43) graft combination, accompanied with a higher survival rate, shoot rate and leaf number of new shoots and cold tolerance in field experiments. Our findings provide a viable tea micrografting method, which has the potential to substitute traditional tea cuttings for tea seedling propagation and thus meet the requirements of tea cultivation.

The 2-oxoglutarate-dependent dioxygenase (2OGD) superfamily is critical for plant primary and secondary metabolism, but its evolutionary dynamics in radish (Raphanus sativus L.) remain uncharacterized. This study identified 165 radish 2OGD members with distinct physicochemical properties, including amino acid lengths ranging from 122 to 546, molecular weights from 13.1 to 60.1 kDa, and predominant subcellular localizations in the cytoplasm, nucleus, and chloroplast. Phylogenetic analysis clustered these 2OGD genes into 20 clades, functionally categorized into groups involved in hormone metabolism, flavonoid biosynthesis, and specialized metabolite synthesis. Chromosomal localization revealed uneven distribution across 9 chromosomes, with 89 pairs of segmental duplicates and significant syntenic relationships with Arabidopsis 2OGD genes, indicating expansion via gene duplication. Two ANS homologs, RsANS1 and RsANS2, in the LDOX clade were highly expressed in red radish taproots, their overexpression in Arabidopsis enhanced anthocyanin content.​ This study clarifies the evolutionary dynamics of the radish 2OGD superfamily, confirms the role RsANS1 and RsANS2 in anthocyanin biosynthesis, and lays a foundation for investigating the functions of 2OGD genes in regulating metabolite diversification and phenotypic development in radish.

Sponge gourd (Luffa cylindrica (L.) Roem.) is a valuable vegetable crop with diverse fruit shapes, yet the differences in metabolites and the underlying molecular mechanisms in fruits with different shapes remain poorly understood. In this study, we integrated morphological, metabolomic and transcriptomic analyses to investigate the growth dynamics, metabolite profiles, and gene expression patterns in two distinct Luffa varieties—‘S’ and ‘L’—which significantly differ in fruit shape and size. Growth monitoring revealed that the ‘L’ variety reached a maximum length 2.2 times that of ‘S’, while the ‘S’ variety developed a diameter 2.4 times larger than ‘L’. Metabolomic profiling identified 1,835 metabolites across 13 categories, revealing significant differences between the two varieties. A total of 470 differentially accumulated metabolites (DAMs) were detected, with phenylpropanoid biosynthesis and starch and sucrose metabolism pathways showing significant enrichment. Transcriptome analysis identified 4,456 differentially expressed genes (DEGs), with marked alterations in the same pathways. In ‘L’ fruits, higher expression of phenylpropanoid-related genes was associated with increased flavonoid accumulation, while in ‘S’ fruits, elevated expression of the INV, glgC and CELB genes correlated with higher levels of D-sucrose and D-trehalose. This study provides novel insights into the molecular mechanisms regulating fruit quality in Luffa and highlights potential targets for genetic improvement.

[Objective] TIFY gene are involved in the regulation of growth and development in plants. This experiment involves the identification of the TIFY family members in Luffa cylindrica, selecting the highly expressed members in fruits, and further cloning and investigating their expression patterns in different tissues, in order to provide basis for the functional identification of these genes. [Methods] Bioinformatics methods were used to identify the TIFY gene family members in L. cylindrica. Transcriptome analysis was performed to screen the highly expressed members in L. cylindrica fruits, followed by cloning genes using RT-PCR. Tissue expression was analyzed using qRT-PCR. [Results] L. cylindrica genome contains 17 TIFY genes, which were unevenly distributed across 9 chromosomes, with CDS lengths ranging from 297 bp to 1 353 bp, classifing into four subfamilies. Transcriptome analysis revealed that LcTIFY2, LcTIFY7, LcTIFY11, and LcTIFY13 were highly expressed in L. cylindrica fruits. The CDS sequences of these genes were cloned, comprising 3, 5, 6, and 2 exons, respectively. Tissue expression analysis indicated that all four genes had the highest expression in the peel of L. cylindrica fruits. Additionally, LcTIFY7 and LcTIFY13 showed significantly higher expression in reproductive organs, while LcTIFY11 exhibited significantly higher expression in female flower buds and ovaries. [Conclusion] The TIFY family members LcTIFY2, LcTIFY7, LcTIFY11 and LcTIFY13 may play roles in reproductive growth and fruit development in L. cylindrica.

Autophagy, a highly conserved cellular process in plants, plays a vital role in regulating abiotic stress responses and senescence. Eggplant is a widely cultivated vegetable crop, its fruit is susceptible to cold damage, and its molecular mechanisms underlying stress tolerance, fruit ripening, and senescence remain largely uncharacterized. In this study, we systematically identified 41 Autophagy-Related Genes (ATGs) in eggplant and through bioinformatics methods, we analyzed their gene structures, evolutionary features, chromosomal locations, and promoter elements. Additionally, we conducted RNA-seq transcriptome analysis of various eggplant organs as well as under different stress conditions, and assessed the expression patterns of the SmATG8 under cold stress in eggplant fruit. Our analysis results show that the SmATGs promoters have diverse cis-regulatory elements, with most SmATGs exhibiting low and stable expression levels in different tissues. Some genes displayed tissue-specific expression patterns. Under 4 °C low-temperature stress, the expression of the SmATG8 family was continuously upregulated, indicating that autophagy plays a crucial role in defending against cold damage in eggplant. Furthermore, we found that ATGs in dicots and monocots exhibit distinct homology patterns. Within the dicots, eggplant and pepper, both being Solanaceae plants, their ATGs exhibit high homology, suggesting that SmATGs in Solanaceae plants have unique homologous relationships. The analysis of SmATGs unveiled their potential contributions to plant stress responses, fruit ripening, and senescence, thus offering a theoretical foundation for further exploration of stress resistance and senescence mechanisms in eggplant.

Peach tree is one of the most important fruit trees in the world, and it has been cultivated for more than 7,500 years. In recent years, the genome and population resequencing of peach trees have been published continuously, which has effectively promoted the research of peach tree genetics and breeding. In order to promote the further mining and utilization of these data, we integrated and constructed a comprehensive peach genome and variation database (PPGV, http://peachtree.work/home ). The PPGV contains 10 sets of published peach tree genome data, as well as genomic variation information for 1,378 peach tree samples (the resequencing data of 1,378 samples were aligned with the high-quality genomes of Lovell, CN14 and Chinesecling, respectively, for mutation detection). A variety of useful and flexible tools, such as BLAST, Gene ID Convert, KEGG/GO Enrichment, Primer Design and Gene function, were also specially designed for searching data and assisting in breeding.

The TBL (Trichome Birefringence-Like) gene family, which participates in the initiation of trichomes and the acetylation of xylan in a variety of plant species, plays a significant role in plant biology. However, there is little information regarding TBL family members in pear (Pyrus bretschneideri Rehd). Here, 65 PbrTBL genes were identified in Pyrus bretschneideri genome. Phylogenetic, gene structure, expression pattern and cis-element of promoter analysis were performed and compared. Expression profiling across different tissues and in response to Botryosphaeria dothidea (B. dothidea) infection highlighted the dynamic and coordinated response of PbrTBL genes, with PbrTBL43 showing significant upregulation. Subcellular localization of PbrTBL43 to the plasma membrane and the enhanced susceptibility to B. dothidea infection upon PbrTBL43 silencing further support its role in pathogen resistance. This study enhances our understanding of the PbrTBL gene family's multifaceted involvement in pear biology and provides a foundation for future research aimed at improving pear resistance to diseases and environmental challenges.

Sugars produced by photosynthesis provide energy for biological activities and the skeletons for macromolecules; they also perform multiple physiological functions in plants. Sugar transport across plasma membranes mediated by the Sugar Will Eventually be Exported Transporter (SWEET) genes substantially affects these processes. However, the evolutionary dynamics and function of the SWEET genes are largely unknown in radish, an important Brassicaceae species. Genome-wide identification and analysis of the RsSWEET genes from the recently updated radish reference genome was conducted using bioinformatics methods. The tissue-specific expression was analyzed using public RNA-seq data, and the expression levels in the bud, stamens, pistils, pericarps and seeds at 15 and 30 days after flowering (DAF) were determined by RT‒qPCR. Thirty-seven RsSWEET genes were identified and named according to their Arabidopsis homologous. They are unevenly distributed across the nine radish chromosomes and were further divided into four clades by phylogenetic analysis. There are 5–7 transmembrane domains and at least one MtN3_slv domain in the RsSWEETs. RNA-seq and RT‒qPCR revealed that the RsSWEETs exhibit higher expression levels in the reproductive organs, indicating that these genes might play vital roles in reproductive organ development. RsSWEET15.1 was found to be especially expressed in siliques according to the RNA-seq data, and the RT‒qPCR results further confirmed that it was most highly expressed levels in the seeds at 30 DAF, followed by the pericarp at 15 DAF, indicating that it is involved in seed growth and development. This study suggests that the RsSWEET genes play vital roles in reproductive organ development and provides a theoretical basis for the future functional analysis of RsSWEETs in radish.

Our previous study found that large-leaf yellow tea (LYT) had interesting hypoglycemic activity in high-fat diet-induced obese mice and highly safety in healthy mice. To study the anti-diabetic potential of LYT, the present study further investigated the preventive effects and mechanisms of action of LYT administration on diabetes and diabetic nephropathy in high-fat diet plus streptozotocin-induced diabetic mice. Results showed that LYT infusions (1/100 and 1/50, m/V) as drinking fluid for 4 weeks reduced diabetic polydipsia and polyuria, enhanced glucose tolerance and insulin sensitivity, and lowered fasting blood glucose level. The underlying mechanisms involve downregulation of gluconeogenesis (lower protein levels of TXNIP and FBP and enzyme activity of FBP), upregulation of lipid catabolism (higher protein levels of CPT-1α and PPARα), downregulation of lipogenesis (lower protein level of SREBP-1), and modification of the structure and abundance of gut microbiota to modulate metabolic homeostasis. Moreover, LYT administration prevented diabetic nephropathy, possibly due to reduced glucose-caused osmotic diuresis and lowered levels of renal PKC-β2, NLRP3 as well as membrane PKC-α, AQP2 and glycosylated AQP2 proteins. Taken together, LYT exhibits the activities in alleviating diabetic symptoms, ameliorating glucose and lipid dysmetabolism and fatty liver, and preventing diabetic nephropathy in diabetic mice. These activities may be explored for the prevention and treatment of diabetes in humans.

IntroductionExcessive calorie intake and physical inactivity have dramatically increased nutrient overload-associated disease, becoming a global public health issue. Chimonanthus salicifolius S. Y. Hu (CHI) is a homology plant of food and medicine in China and shows several health benefits. MethodsThis work investigated the antioxidant activity, the alleviating effects, and the mechanism of action on diabetes and hyperlipidemia of CHI leaves. Results and discussionResults showed that CHI leaves infusion displayed in vitro antioxidant activity measured by ABTS and ferric reducing antioxidant power methods. In wild-type Kunming mice, CHI leaves infusion consumption activated the hepatic antioxidant enzymes, including glutathione reductase, glutathione S-transferase, glutathione peroxidase and thioredoxin reductase as well as thioredoxin reductase 1. In alloxan-induced type 1 diabetic mice, CHI leaves infusion ameliorated diabetic symptoms, including polyuria, polydipsia, polyphagia and hyperglycemia, in a dose-dependent and time-course manners. The mechanism involved CHI leaves up-regulating renal water reabsorption associated protein - urine transporter A1-and promoting the trafficking of urine transporter A1 and aquaporin 2 to the apical plasma membrane. Despite this, in high-fat diet-induced hyperlipidemic golden hamsters, CHI leaves powder did not significantly effect on hyperlipidemia and body weight gain. This might be attributed to CHI leaves powder increasing the calorie intake. Interestingly, we found that CHI leaves extract containing a lower dose of total flavonoid than CHI leaves powder pronouncedly reduced the levels of total cholesterol, triglyceride, and low-density lipoprotein cholesterol in serum in golden hamsters fed a high-fat diet. Furthermore, CHI leaves extract elevated the diversity of gut microbiota and the abundance of Bifidobacterium and Ruminococcaceae_UCG-014. It also decreased the abundance of Lactobacillus at the genus level in golden hamsters fed a high-fat diet. Overall, CHI leaves benefit oxidative stress prevention and metabolic syndrome amelioration in vivo.

Cold stress is one of the major abiotic stresses limiting tea production. The planting of cold-resistant tea cultivars is one of the most effective measures to prevent chilling injury. However, the differences in cold resistance between tea cultivars remain unclear. In the present study, we perform a transcriptomic and metabolomic profiling of Camellia sinensis var. "Shuchazao" (cold-tolerant, SCZ) and C. sinensis var. assamica "Yinghong 9" (cold-sensitive, YH9) during cold acclimation and analyze the correlation between gene expression and metabolite biosynthesis. Our results show that there were 51 differentially accumulated metabolites only up-regulated in SCZ in cold-acclimation (CA) and de-acclimation (DA) stages, of which amino acids accounted for 18%. The accumulation of L-arginine and lysine in SCZ in the CA stage was higher than that in YH9. A comparative transcriptomic analysis showed an enrichment of the amino acid biosynthesis pathway in SCZ in the CA stage, especially "arginine biosynthesis" pathways. In combining transcriptomic and metabolomic analyses, it was found that genes and metabolites associated with amino acid biosynthesis were significantly enriched in the CA stage of SCZ compared to CA stage of YH9. Under cold stress, arginine may improve the cold resistance of tea plants by activating the polyamine synthesis pathway and CBF (C-repeat-binding factor)-COR (cold-regulated genes) regulation pathway. Our results show that amino acid biosynthesis may play a positive regulatory role in the cold resistance of tea plants and assist in understanding the cold resistance mechanism differences among tea varieties.

Increasing evidence shows that selenium and polyphenols are two types of the most reported compounds in tumor chemoprevention due to their remarkable antitumor activity and high safety profile. The cross-talk between polyphenols and selenium is a hot research topic, and the combination of polyphenols and selenium is a valuable strategy for fighting cancer. The current work investigated the combination anti-peritoneal carcinomatosis (PC) effect of selenium nanoparticles (SeNPs) and green tea (Camellia sinensis) polyphenol (-)-epigallocatechin-3-gallate (EGCG) in mice bearing murine hepatocarcinoma 22 (H22) cells. Results showed that SeNPs alone significantly inhibited cancer cell proliferation and extended the survival time of mice bearing H22 cells. Still, the potential therapeutic efficacy is accompanied by an approximately eighty percent diarrhea rate. When EGCG was combined with SeNPs, EGCG did not affect the tumor proliferation inhibition effect but eliminated diarrhea triggered by SeNPs. In addition, both the intracellular selectively accumulated EGCG without killing effect on cancer cells and the enhanced antioxidant enzyme levels in ascites after EGCG was delivered alone by intraperitoneal injection indicated that H22 cells were insensitive to EGCG. Moreover, EGCG could prevent SeNP-caused systemic oxidative damage by enhancing serum superoxide dismutase, glutathione, and glutathione peroxidase levels in healthy mice. Overall, we found that H22 cells are insensitive to EGCG, but combining EGCG with SeNPs could protect against SeNP-triggered diarrhea without compromising the suppressing efficacy of SeNPs on PC in mice bearing H22 cells and attenuate SeNP-caused systemic toxicity in healthy mice. These results suggest that EGCG could be employed as a promising candidate for preventing the adverse reactions of chemotherapy including chemotherapy-induced diarrhea and systemic toxicity in cancer individuals.

Accumulated evidence shows that melatonin possesses the potential to improve lipid metabolism by modifying gut microbiota and glucose metabolism via regulating the melatonin receptor signaling pathway. However, the contribution of melatonin consumption on glucose homeostasis by affecting gut microbiota has not been investigated in diabetes. In the current work, we investigated the effect of melatonin administration on gut microbiota and glucose homeostasis in db/db mice, a type 2 diabetes model with leptin receptor deficiency. Administration of melatonin through drinking water (at 0.25% and 0.50%) for 12 weeks decreased diabetic polydipsia and polyuria, increased insulin sensitivity and impeded glycemia. The accumulated fecal levels of total short-chain fatty acids (SCFAs) and acetic acid are positively correlated with diabetes-related parameters-homeostasis model assessment of insulin resistance (HOMA-IR) index and fasting blood glucose (FBG) level. The reprogramming of gut microbiota structure and abundance and the reduction of fecal levels of SCFAs, including acetic acid, butyric acid, isovaleric acid, caproic acid, and isobutyric acid, by melatonin may be beneficial for enhancing insulin sensitivity and lowering FBG, which were verified by the results of correlation analysis between acetic acid or total SCFAs and HOMA-IR and FBG. In addition, the melatonin downregulated hepatic genes, including fructose-1,6-bisphosphatase 1, forkhead box O1 alpha, thioredoxin-interacting protein, phosphoenolpyruvate carboxy-kinase (PEPCK), PEPCK1 and a glucose-6-phosphatase catalytic subunit, that responsible for gluconeogenesis support the result that melatonin improved glucose metabolism. Overall, results showed that the melatonin supplementation reduced fecal SCFAs level via reprogramming of gut microbiota, and the reduction of fecal SCFAs level is associated with improved glucose homeostasis in db/db mice.

BACKGROUND:Sugar not only is an important biomacromolecule that plays important roles in plant growth, development, and biotic and abiotic stress tolerance but also provides a skeleton for other macromolecules, such as proteins and nucleic acids. Sugar transporter proteins (STPs) play essential roles in plant sugar transport and ultimately affect the abovementioned life processes. However, the evolutionary dynamics of this important gene family in Brassicaceae crops are still largely unknown, and the functional differentiation of radish STP genes remains unclear.RESULTS:In the present study, a comparative genomic study of STP genes in five representative Brassicaceae crops was conducted, and a total of 25, 25, 28, 36 and 49 STP genes were individually identified in Raphanus sativus (Rs), Brassica oleracea (Bo), B. rapa (Br), B. napus (Bn) and B. juncea (Bj), which were divided into four clades by phylogenetic analysis. The number of STP genes was no direct correlation with genome size and the total number of coding genes in Brassicaceae crops, and their physical and chemical properties showed no significant difference. Expression analysis showed that radish STP genes play vital roles not only in flower and seedpod development but also under heavy metal (cadmium, chromium and lead), NaCl and PEG-6000 stresses, Agrobacterium tumefaciens infection, and exogenous sugar treatment. RsSTP13.2 was significantly upregulated in the resistant radish cultivar by A. tumefaciens infection and induced by heavy metal, NaCl and PEG-6000 stress, indicating that it is involved in resistance to both biotic and abiotic stress in radish.CONCLUSIONS:The present study provides insights into the evolutionary patterns of the STP gene family in Brassicaceae genomes and provides a theoretical basis for future functional analysis of STP genes in Brassicaceae crops.

基于中国知网(CNKI)中国期刊全文数据库,检索年份为2001-2019年,这19年间有关茶叶抗氧化研究论文,对发文量、期刊、作者、机构、学科进行文献计量学统计分析,并综述分析研究热点.此为茶叶抗氧化的深入研究提供新的思路和方法.

CBFs play important roles in tea plant cold tolerance. In our study, 16 tea varieties were used to investigate the relationship between the expression level of CsCBFs and cold tolerance in field experiments. A strong and positive correlation was found between cold stress-regulated CsCBF1, CsCBF3 and CsCBF5 expression levels (R-2 > 0.8) in tea mesophyll cells and cold tolerance in 16 tea varieties. A previous study reported that CsCBF1 and CsCBF3 were important components associated with cold tolerance in tea plants; thus, the function of CsCBF5 in the CsCBF family was targeted. Our previous study reported that CsCBF5 was localized in the nucleus and exhibited transcriptional activity. In the current study, MDA content in leaves was significantly increased in CsCBF5-silenced leaves, which exhibited poor cold tolerance, compared with WT plants under cold stress. In contrast, increased germination rates and antioxidant enzyme activities under cold conditions compared with WT plants. Furthermore, CsCBF5 overexpression in Arabidopsis promoted the expression levels of the cold-regulated genes AtCOR15a, AtCOR78, AtERD4 and AtRD29B; however, the expression levels of downstream genes, including CsCOR47, CsCOR413, CsERD4 and CsRD29B, were significantly reduced in CsCBF5-silenced tea leaves. Taken together, our results indicated that CsCBF5 could function as a positive regulator in the cold stress response.