Effect of ants Formica rufa was studied on the fed and unfed stages of the ticks Ixodes persulcatus and Dermacentor nuttalli in vivo. The unfed and the fed tick stages of D. nuttalli had a higher survival than the unfed and the fed tick stages of I. persulcatus. 85 % formic acid causes death of ixodes ticks within 5 minutes, 60 % formic acid – within 10 minutes. Ants F. rufa prey on ixodes ticks and can reduce their populations. Ants F. rufa can be used as a biological control for ixodes ticks in small territories.

Contamination of dogs with Toxocara canis and efficiency of prevention measures of toxocarosis in cynological center of Altai Republic is studied.

The epidemiological and epizootiological features of toxocariasis foci were investigated in the Republic of Altai. The results of coproovoscopic examinations of dogs, those of serological screening for toxocariasis among different age and occupational groups and coniingents at risk for HIV infection, hepatitis B and C, patients with allergic and bronchopulmonary diseases, toxocariasis mortality rates in the Republic of Altai, and the materials of sanitary and parasitological studies of soil and other environmental constituents for Toxocara canis eggs provide support for the poor situation of toxocariasis in the region. Relying on the current standards and the findings of this study, the Republic of Altai may be referred to as a moderately dangerous area at medium risk for contamination in terms of epidemic, epizootic, and environmental hazards. The results of assessing the quality of laboratory diagnosis of zoonotic helminthisms call for further improvement of the professional knowledge, abilities, and skills of the employees from health care and veterinary facilities, as well as economic objects within production laboratory control, basing on the principles and requirements of the environmental management system and the food quality and safety management system.

One investigated the sanitary-parasitological and epizootological peculiarities of T. canis infection in the Republic of Altai. The results of coproovoscopic examination of dogs, sanitary-parasitological testing of soil, water of open reservoirs and production of plant origin confirmed the high incidence of T. canis infection in that region. Based on the obtained data and existing standards the Republic of Altai in respect of epidemic hazard of environment (soil) could be attributed to the category of “moderately hazardous” territory.

The time of development and survival of Toxocara canis eggs in the soil of Gorno-Altaisk was experimen- tally investigated in 2011-2014. In July 2011, T.canis eggs matured at 3-5-cm soil depths from the stage of 1-2 blastomeres to invading larva within 12 days at a daily average temperature of +23°C and at a relative humidity of 82%. At 3-5-cm soil depths, more than 70% of invaded T. canis eggs preserved their viability through- out the experimental period (4 years). The paper gives evidence for the seasonal survival of invaded T. canis eggs in relation of the length of soil stay.

Serologic testing of Lyme disease, human granulocytic anaplasmosis and human monocytotopic ehrlichiosis, showed the prevalence of these infections and the risk of mixed infection. The patients with tick-borne encephalitis were found to have its monoinfection and mixed infection in 66.1% and 33.9% of cases, respectively.

The authors investigated the effects of disinfectants, such as desavid (brand name Desavid), delegol, DP-2T, sulfochloranthione, and hydrogen peroxide on Toxocara canis eggs in the pure culture, Desavid BAS on them in the household-fecal sludge, and sodium hypochlorite in the soil cover. Only desavid (brand name Desavid), delegol, and sodium hypochlorite have a marked ovicidal effect.

Contamination of dogs with Toxocara canis and efficiency of prevention measures of toxocarosis in cynological center of Altai Republic is studied

Six household chemicals were tested for effects on cultured Toxocara canis eggs in the mobile larval stage. The cleansing gel Comet (Double Effect) and its 10%, 25%, and 50% aqueous solutions have the most pronounced ovicidal and larvicidal effects under laboratory conditions (+19-22 degrees C). Domestos and Belizna (Bleach) are also effective. These agents are recommended for the disinvasion and washing of premises for canids: aviaries of dog-breeding centers; farms for silver and polar foxes; shelters for stray dogs; rooms in veterinary clinics; and apartments where domestic animals (dogs) are present.

The insectoacaricid Briz 25% e.c. was tested for its effects on Toxocara canis eggs in pure culture. In the solar-illuminated laboratory at 22-23°C, T. canis eggs of pure culture were destroyed on days 17-18 when in 1.25% aqueous emulsion of the insectoacaricid Briz 25% e.c. (0.313% with reference to the active ingredient cypermethrin). Within the egg, the parasite failed to die not only in the stage of protoblast (5-6 days), but also in the stages of semiformed (4 days) and mobile larva (7-8 days), i.e. for 16-17 days of the experiment. After eggshell destruction, T. canis larvae actively entered the working aqueous emulsion of Briz 25% e.c. and died within the first hours in the test agent. The working aqueous emulsion of Briz 25% e.c. (1.25% with reference to the agent or 0.313% to the active ingredient cypermethrin) exerted ovicidal and larvicidal activities against the pathogen of T. canis in the pure culture on days 17-18 at 22-23°C.