Since chronic inflammation is a typical feature of polycystic ovary syndrome (PCOS), both clinical and experimental studies have demonstrated that resveratrol (RES) can effectively alleviate it. However, the underlying mechanism remains unclear. To further investigate this, granulosa cells (GCs) derived from PCOS patients, lipopolysaccharide (LPS)-treated human granulosa cells (KGN), LPS-induced chronic inflammation mouse models, and dehydroepiandrosterone (DHEA)-induced PCOS mouse models were treated with RES. The expression of inflammatory cytokines, including interleukin (IL)-6, IL-1β, chemoattractant protein-1 (MCP-1), and cyclooxygenase-2 (COX2), as well as absent in melanoma 2 (AIM2), was examined. Additionally, ovarian morphological changes in these mouse models were assessed using hematoxylin-eosin (HE) staining. The results showed that the expression of these inflammatory cytokines and AIM2 increased significantly in GCs derived from PCOS patients and LPS-induced KGN cells, as well as in the ovaries of LPS-induced chronic inflammation and DHEA-induced PCOS mouse models. Furthermore, blocking AIM2 in LPS-treated KGN cells and mice with LPS-induced inflammation or PCOS significantly reduced the upregulation of inflammatory cytokines, similar to the results observed following RES treatment. In addition, LPS-induced phosphorylation of the JAK2/STAT3 pathway in KGN cells was completely abolished by RES treatment. Notably, LPS-induced upregulation of AIM2 and these inflammatory cytokines was completely reversed by blocking the JAK2/STAT3 pathway using AZD-1480 and SH-4-54, respectively. Further in vivo studies showed that ovarian morphological and estrous cycle disturbances in DHEA-induced PCOS mouse models were effectively ameliorated by RES and A151. In conclusion, RES alleviates chronic inflammation in PCOS by inhibiting AIM2 via blocking the JAK2/STAT3 pathway. Our findings suggest that the targeted inhibition of AIM2 could represent a novel therapeutic approach for PCOS.

Ovulatory dysfunction is a typical symptom of polycystic ovary syndrome (PCOS) and is widely recognized as a major obstacle to fertility. Numerous studies have reported a close association between the programmed cell death (PCD) of granulosa cells (GCs) and ovulatory dysfunction in PCOS. Recently, a novel type of PCD exhibiting features of pyroptosis, apoptosis, and necroptosis was identified and termed PANoptosis. However, whether the PANoptosis occurs in PCOS GCs and plays a role in ovulatory dysfunction remains unclear. In this study, we found that the expression levels of PANoptosis-related molecules (NLRP3, CASP1, IL-1β, CASP3, MLKL), the PANoptosis regulator AIM2, IFN-γ, the IFN-responsive transcription factor 1 (IRF1), and HAS2 were significantly increased in GCs collected from PCOS patients, DHT-treated KGN cells, and the ovaries of DHEA-induced PCOS mouse models. Most importantly, KGN cells treated with a combination IFN-γ and lipopolysaccharide (LPS) exhibited similar effects. While, blocking IRF1 (using RNA interference-based knockdown), HAS2 (using 4-MU), JAK2 (using AZD-1480), or STAT3 (using SH-4-54) completely abolished these effects. Mechanistically, we revealed that elevated IFN-γ promotes AIM2 expression and AIM2-PANoptosome assembly through the JAK2/STAT3/IRF1/HAS2 axis. Further in vivo studies showed that inhibiting HAS2 (using 4-MU) and AIM2 (using A151) increased the number of oocytes retrieved from DHEA-induced PCOS mice. Taken together, our results suggest that AIM2-PANoptosis occurs in the GCs of PCOS and is triggered by the IFN-γ/IRF1/HAS2 signalling pathway, thereby resulting in ovulatory dysfunction. This study therefore provides valuable potential therapeutic targets for the treatment of this typical PCOS symptom.

The proliferation and migration of porcine trophectoderm (pTr) cells are crucial processes during the early stages of embryo implantation in sows. The effects of deoxynivalenol (DON) and chlorogenic acid (CGA), a plant-derived compound, on pTr cells are currently unclear. In this study, pTr cells were treated with DON at different times (24, 48, and 72 h) and different concentrations (0.5, 1, and 2 μg/mL) to construct a pathological model of DON-induced pTr cells by detecting the expression levels of genes related to cell proliferation, migration, and oxidative stress, as well as the cell viability and the cell migration ability. Subsequently, CGA intervention experiments revealed that CGA could promote the proliferation, migration, and antioxidant ability of pTr cells and alleviate the damage induced by DON in pTr cells. Finally, RNA-seq technology combined with experiments illustrated that CGA might alleviate the damage of DON-induced pTr cells by regulating the PI3K/AKT signaling pathway. In conclusion, this study explored the toxicological effect of DON and the alleviation effect of CGA on DON at the pTr cells level, which provided new insights and an experimental basis for using CGA to alleviate the reproductive toxicity induced by DON.

Kynurenine (KYN) is a primary tryptophan derivative found in the human body and fermented foods. Previous studies have shown that KYN is an aryl hydrocarbon receptor (AHR) agonist and is important in regulating various physiological activities, including female reproduction. Progesterone is a vital steroid hormone that facilitates embryo implantation and maintains pregnancy. However, whether KYN affects its biosynthesis remains unclear. To gain understanding, in vitro luteinized porcine granulosa luteal (pGL) cells were treated with KYN. The results showed that KYN disrupted progesterone biosynthesis by decreasing the expression of steroidogenic acute regulatory protein (STAR) and 3beta-hydroxysteroid dehydrogenase (HSD3B) in pGL cells. In addition, the expression of three transcription factors of STAR and HSD3B (GATA4, GATA6, and CEBPB) decreased after KYN treatment. Furthermore, the AHR blockade results showed comparable effects to those of KYN treatment, and subsequent knockdown experiments confirmed these results. These findings suggest that KYN inhibits progesterone biosynthesis in pGL cells by downregulating GATA4, GATA6, and CEBPB expression through AHR. Thus, our results showed for the first time a previously unknown connection between KYN and progesterone biosynthesis.

Mi pigs, a Chinese native breed found in Jintan and Yangzhong in Jiangsu Province, were recorded as having only a few hundred members in the latest national livestock and poultry genetic resources survey. To explore their conservation and breeding prospects, 18 SINE Retrotransposon Insertion Polymorphisms (sine-rips) were analyzed using PCR to assess the population structure and genetic diversity of Mi pigs. These pigs were grouped into eight families based on a UPGMA phylogenetic tree. The genetic distances between the Mi pig populations and commercial breeds ranged from 0.3712 to 0.7609, indicating significant divergence. Conversely, they showed a closer genetic relationship with other local Jiangsu breeds, with distances varying from 0.0943 to 0.6122, a finding supported by the UPGMA tree. The populations displayed a substantial degree of outbreeding, with Fis values from -0.4744 (M5) to -0.0847 (M8) and Fst values from 0.0534 (M3, M8) to 0.2265 (M2, M7), highlighting their genetic diversity which is crucial for the conservation of Mi pigs. Despite this diversity, the population sizes were uneven, with M5, M7, and M8 having 6, 5, and 7 individuals, respectively. These findings lay a theoretical foundation for the ongoing conservation and breeding efforts for Mi pigs.

Abstract Kynurenine (KYN) is a primary tryptophan derivative found in the human body and fermented foods. Previous studies have shown that KYN is an Aryl hydrocarbon receptor (AHR) agonist and is important in regulating various physiological activities, including female reproduction. Progesterone is a vital steroid hormone that facilitates embryo implantation and maintains pregnancy. However, whether KYN affects its biosynthesis remains unclear. To gain understanding, in vitro luteinized porcine granulosa luteal (pGL) cells were treated with KYN. The results showed that KYN disrupted progesterone biosynthesis by decreasing the expression of STAR and HSD3B in pGL cells. In addition, the expression of three transcription factors of STAR and HSD3B (GATA4, GATA6, and CEBPB) decreased after KYN treatment. Furthermore, the AHR blockade results showed comparable to those of KYN treatment, and subsequent knockdown experiments confirmed these results. These findings suggest that KYN inhibits progesterone biosynthesis in pGL cells by downregulating GATA4, GATA6, and CEBPB expression through AHR. Thus, our results showed for the first time, a previously unknown connection between KYN and progesterone biosynthesis.

BackgroundPolycystic ovary syndrome (PCOS) is a heterogeneous metabolic and endocrine disorder that causes anovulatory infertility and abnormal folliculogenesis in women of reproductive age. Several studies have revealed inflammation in PCOS follicles, and recent evidence suggests that Berberine (BBR) effectively reduces inflammatory responses in PCOS, however, the underlying mechanisms remain unclear.PurposeTo determine the underlying mechanisms by which BBR alleviates inflammation in PCOS.Study designPrimary human GCs from healthy women and women with PCOS, and KGN cells were used for in vitro studies. ICR mice were used for in vivo studies.MethodsGene expression was measured using RT-qPCR. HAS2, inflammatory cytokines, and serum hormones were assayed by ELISA. Protein expression profiles were assayed by Western blot. Chronic low-grade inflammatory mouse models were developed by intraperitoneal injection with LPS, and PCOS mouse models were established by subcutaneous intraperitoneal injection of DHEA. BBR and 4-MU were administered by gavage. Ovarian morphologic changes were evaluated using H&E staining. HAS2 expression in the ovary was assayed using Western blot and immunohistochemistry.ResultsOur results confirmed that HAS2 expression and hyaluronan (HA) accumulation are closely associated with inflammatory responses in PCOS. Data obtained from in vitro studies showed that HAS2 and inflammatory genes (e.g., MCP-1, IL-1β, and IL-6) are significantly upregulated in PCOS samples and LPS-induced KGN cells compared to their control groups. In addition, these effects were reversed by blocking HAS2 expression or HA synthesis using BBR or 4-MU, respectively. Furthermore, HAS2 overexpression induces the expression of inflammatory genes in PCOS. These results were further confirmed in LPS- and DHEA-induced mouse models, where inflammatory genes were reduced by BBR or 4-MU, and ovarian morphology was restored.ConclusionsOur results define previously unknown links between HAS2 and chronic low-grade inflammation in the follicles of women with PCOS. BBR exerts its anti-inflammatory effects by down-regulating HAS2. This study provides a novel therapeutic target for alleviating ovarian inflammation in women with PCOS.

肌 内 脂 肪(Intramuscular Fat, IMF)是指肌肉样品中通过化学方法提取的脂肪,是嵌入在猪肌肉纤维之间的脂肪组织,主要来源于脂肪细胞和肌细胞.肌内脂肪是猪肉品质的重要指标,是影响猪肉品质的重要性状之一,与风味、多汁性、嫩度等密切相关,决定猪肉的品质和营养价值.肌内脂肪含量较高时,猪肉具有更好的嫩度、多汁性和适口性.十几年来,养猪生产中由于对较高瘦肉率和较低背膘厚的追求,商品猪肌内脂肪含量一直在下降.胴体瘦肉率每提高一个百分点,肌内脂肪含量降低0.07％.肌内脂肪的沉积受多种因素调控,不同品种猪在脂肪代谢相关酶活性、相关基因表达量等方面均存在较大差异.通过改变日粮中能量、蛋白质以及脂肪酸比例,肌内脂肪也会受到不同程度的影响.进一步研究发现,脂肪调控基因通过调控脂代谢与脂生成的过程调控肌内脂肪沉积的进程.为了适应消费者对猪肉品质的需求,研究猪肌内脂肪沉积的相关机制,寻找提高肌内脂肪含量的策略,对优质猪肉的生产至关重要.

集约化畜禽场的臭气排放对环境和人类健康的危害引发越来越多的关注.臭气处理的方法有多种,其中生物法因其低成本、除臭效率高的优势得到了更为广泛的应用.在综述不同生物除臭技术原理的基础上,对影响除臭性能的主要因素(填料、pH、营养、温度、微生物)进行分析.总结新型生物除臭设备及技术的研究进展,并对今后生物除臭的发展趋势进行展望.

Lipopolysaccharide (LPS), also known as endotoxin, is a component of the outer membrane of gram-negative bacteria. LPS is released into the surrounding environment during bacterial death and lysis. Due to its chemical and thermal stability, LPS can be detected anywhere and easily exposed to humans and animals. Previous studies have shown that LPS causes hormonal imbalances, ovarian failure, and infertility in mammals. However, the potential mechanisms remain unclear. In this study, we investigated the effects and mechanisms of LPS on tryptophan degradation, both in vivo and in vitro. The effects of kynurenine, a tryptophan derivative, on granulosa cell function and reproductive performance were explored. Results showed that p38, NF-κB, and JNK signaling pathways were involved in LPS-induced Ido1 expressions and kynurenine accumulation. Furthermore, the kynurenine decreased estradiol production, but increased granulosa cell proliferation. In vivo, experiments showed that kynurenine decreased estradiol and FSH production and inhibited ovulation and corpus luteum formation. Additionally, pregnancy and offspring survival rates decreased considerably after kynurenine treatment. Our findings suggest that kynurenine accumulation disrupts hormone secretion, ovulation, corpus luteal formation, and reproductive performance in mammals.

脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)是一种常见的污染粮食、饲料和食品的霉菌毒素,严重影响人和牲畜的健康.猪对DON非常敏感,DON可造成猪拒食、生长抑制、呕吐以及器官损伤、细胞毒性、免疫毒性等毒害作用.本文主要综述了 DON对猪的毒害作用、毒理机制以及解毒剂开发等方面的研究进展,尤其关注DON毒理作用的遗传调控研究,旨在为从遗传本质提高猪对脱氧雪腐镰刀菌烯醇的抵抗力提供更全面的参考.

为筛选猪总产仔数和产活仔数性状关键SNP分子标记或候选基因,采用Illumina porcine 50K芯片对同一猪场186头加系大白猪能繁母猪开展了全基因组SNP扫描,后又对扫描结果进行了质量控制、Beagle填充和SNP基因型分型,结合这些试验猪的表型性状测定和群体结构分析结果,进行全基因组关联分析(GWAS).群体结构分析结果表明,试验所用的加系大白猪样本未出现群体分层现象;在18对(36条)常染色体上一共得到36867个有效SNP标记,用于试验猪的GWAS分析.GWAS结果表明,这些加系大白猪所有胎次总产仔数共显著关联到2个SNP,分别为seq-rs323899658和seq-rs329781338,其中seq-rs329781338 SNP又注释到3个基因,分别为SSBP1、WEE2和KIAA1147.产活仔数共显著关联到7个SNP,分别为seq-rs81238474、seq-rs321377412、seq-rs340736313、seq-rs80782154、seq-rs81244816、seq-rs81467772和seq-rs329781338,这7个SNP中有5个SNP有基因注释,共注释到22个基因,分别为E-phA1、TAS2R60、FAM131B、CLCN1、CASP2、TMEM139、TRBV21OR9-2、PRSS2、TRBV19、TRBV24-1、U6、SSBP1、WEE2、KI-AA1147、NKX2-8、ALKBH3、HSD17B12、U6、HOMER2、WHAMM、FSD2和SCARNA15.候选基因GO功能和KEGG通路分析结果表明,这些基因GO功能中最显著的生物学过程为多生物过程的调控、分子功能为非跨膜/跨膜蛋白酪氨酸激酶活性,KEGG通路为错配修复.结合GWAS和基因功能注释结果,WEE2和EphA1基因可作为提高加系大白猪总产仔数和产活仔数的关键候选基因.

Type I collagen is the most abundant extracellular matrix (ECM) protein in the mammalian ovary, and comprises two COL1A1 subunits and one COL1A2 subunit. Matrix metalloproteinase 1 (MMP1) is a typical collagenase of type I collagen, that can be detected in ovarian follicles and early corpus luteum. Previous studies demonstrated that MMP1-mediated degradation of type I collagen plays a functional role in regulating corpus luteum formation, and transforming growth factor β1 (TGF-β1) inhibits luteinization and progesterone production in granulosa cells (GCs). Whether TGF-β1 regulates the expression of MMP1, COL1A1, or the deposition of type I collagen during corpus luteum formation remains to be elucidated. This study aimed to investigate the molecular mechanisms through which TGF-β1 regulates MMP1 expression and type I collagen deposition in GCs. Our results show that TGF-β1 upregulates COL1A1 expressions and downregulates MMP1 expression. Inhibition approaches, including pharmacological inhibitors such as p38 inhibitor (SB203580), ERK1/2 inhibitor (U0126), AKT inhibitor (LY294002), and GSK-3β inhibitor (LiCl), as well as knockdown using siRNA specific to these genes, were used. Our results suggest that TGF-β1 decreases MMP1 production via an ALK5-mediated AKT/GSK-3β-dependent signaling pathway, and a decrease in MMP1 levels and an increase in COL1A1 levels synergistically promote type I collagen deposition in GCs. Collectively, these findings provide novel insights into the underlying molecular mechanisms by which TGF-β1 upregulates type I collagen deposition in GCs.

本试验旨在研究放牧对育肥后期苏山猪生长性能、胴体性状以及肌肉中氨基酸和脂肪酸含量的影响.选取健康、体重为(75.0±2.0)kg的苏山猪80头(公母各占1/2),随机分为放牧组和对照组,每组2个重复,每个重复20头.放牧组猪群每天11:00—17:00在果园中放牧,自由采食黑麦草、俄罗斯草等青绿饲料.对照组猪群舍饲.预试期7 d,正试期30 d.结果表明:1)放牧组平均日增重和屠宰率均低于对照组,差异达到显著水平(P<0.05);放牧组胴体骨率高于对照组,差异达到极显著水平(P<0.01).2)放牧组背最长肌失水率低于对照组,差异达到极显著水平(P<0.01);亮度(L?)值和红度(a?)值高于对照组,黄度(b?)值低于对照组,但差异均未达到显著水平(P>0.05).3)放牧组背最长肌中不饱和脂肪酸含量高于对照组,总饱和脂肪酸含量低于对照组,但差异均未达到显著水平(P>0.05).放牧组背最长肌中缬氨酸含量高于对照组,胱氨酸含量低于对照组,差异均达到极显著水平(P<0.01).4)放牧组背最长肌中总必需氨基酸、总鲜味氨基酸和总氨基酸含量均低于对照组,但差异未达到显著水平(P>0.05).放牧组背最长肌中棕榈油酸含量高于对照组,差异达到显著水平(P<0.05);月桂酸、硬脂酸、花生酸等饱和脂肪酸含量低于对照组,且差异均达到极显著水平(P<0.01).综上所述,育肥后期苏山猪进行放牧对其生长性能影响不显著,但对其胴体性状和肌肉品质具有一定程度的改善作用,是生产优质猪肉的一种重要养殖模式.

花生藤粉是花生收获后形成的一种农副产品,含有丰富的粗蛋白质、粗脂肪、碳水化合物、粗纤维等营养物质.花生藤粉不仅营养丰富,而且质地松软,可以作为一种优质的畜禽饲料原料.花生藤粉主要用于牛、兔、羊、鹅等草食动物生产中,通过合理的调制、微生物发酵等,可以提高饲料利用率,有利于草食动物生长发育和肌肉品质的改善.近年来生产中发现,花生藤粉也可作为一种粗纤维饲料应用于养猪生产中,但添加比例和应用范围尚不明确,需要进行深入研究.

Cholesterol is a precursor to steroid hormones and can be obtained from serum LDL or de novo synthesis in steroidogenic cells. Before luteinizing hormone (LH) surge-induced ovulation, follicles remain avascular, and cholesterol required for progesterone production in granulosa cells (GCs) is derived from de novo biosynthesis. Previous studies have verified that the intrafollicular TGF-β1 plays inhibitory roles in GCs luteinization, vascularization, and progesterone production. Nevertheless, the regulatory function of TGF-β1 on de novo cholesterol synthesis in granulosa-lutein (GL) cells remains largely unknown. We aim to investigate this aspect in this study using in vivo cultured human GL cells. Our results suggested that TGF-β1 significantly suppresses intracellular cholesterol levels and down-regulates the expression of the final step enzyme, DHCR24, that catalyzes de novo cholesterol synthesis. We used specific inhibitors and siRNA-mediated knockdown approaches demonstrate that TGF-β1 suppression of DHCR24 expression in GL cells is mediated by the GSK-3β/EZH2/H3K27me3 signaling pathway. Further ChIP assays revealed that elevated H3K27me3 levels in the promoter region of DHCR24 play a vital role in TGF-β1-induced DHCR24 down-regulation, and RNA-sequencing results confirmed these findings. Notably, our study provides a novel insight into the molecular mechanisms by which TGF-β1 suppresses de novo cholesterol biosynthesis in GL cells.

旨在利用YE1-BE3-FNLS载体对猪的CD163基因进行C＞T的单碱基突变,形成终止密码子TAA,从而导致CD163基因翻译的提前终止.利用网站在猪CD163基因的第7外显子筛选到高效率的sgRNA序列使其符合C5位点,并且C5后续的两个碱基为A A,CAA与起始密码子ATG之间的碱基数为3的整数倍.PCR扩增CD163-sgRNA 和 YE1-BE3-FNLS 载体中的 APOBEC-Cas9n-UGI 片段,通过体外转录形成 CD163-sgRNA 和APOBEC-Cas9n-UGI mRNA.体外培养猪卵母细胞并进行孤雌激活,利用显微操作仪对孤雌胚胎进行CD163-sgRNA和APOBEC-Cas9n-UGI mRNA注射,待胚胎发育至桑椹胚和囊胚阶段,提取胚胎基因组DNA对单碱基编辑区域进行PCR扩增测序.结果显示,在49个候选sgRNA中成功筛选出一个CD163-sgRNA,利用PCR成功扩增了 CD163-sgRNA 和 APOBEC-Cas9n-UGI 片段,注射 CD163-sgRNA 和 APOBEC-Cas9n-UGI mRNA 后的猪孤雌胚胎发育到2～4细胞期.挑选注射的20个2～4细胞期胚胎经过PCR扩增与产物测序后发现有12个胚胎的CD163基因的第7外显子的预期位点C(num1479)＞T,单碱基编辑效率约60％.对CD163-sgRNA的off-tar-get 分析发现,在错配3个碱基的情况下该CD163-sgRNA有5个off-target区域,对这5个区域进行PCR扩增和sanger测序分析后发现都没有发生C＞T的突变.本研究利用YE1-BE3-FNLS工具在胚胎水平上对猪的CD163基因进行了单碱基编辑,成功使得该基因第7外显子预期位点(num1479)C变为T,从而使得密码子CAA变为TAA,可提前终止CD163基因的翻译.

Mycoplasma hyopneumoniae (M. hyopneumoniae, Mhp) is the causative agent of mycoplasma pneumonia of swine (MPS). M. hyopneumoniae infection causes inflammation in pigs and leads to considerable economic losses in the pig industry. Pregnane X receptor (PXR) is a pluripotent gene regulatory protein that plays an important role in regulating cytochrome P-450 (CYP) in pigs in the context of inflammatory responses, drug metabolism, homeostasis, etc. We previously reported that cytochrome P450 3A29 (CYP3A29) expression was significantly upregulated in pigs infected with M. hyopneumoniae compared with healthy control pigs. This experiment mainly focused on identifying the role of PXR in the regulation of CYP3A29 and inflammatory factors after M. hyopneumoniae infection by establishing pig alveolar macrophage (PAM) cells in which PXR was overexpressed or silenced. Our results showed that the overexpression of PXR could significantly improve the protein and the mRNA expression levels of CYP3A29 with and without M. hyopneumoniae infection in PAM cells. After the expression of PXR was inhibited, protein and mRNA expression levels of CYP3A29 were significantly reduced with and without M. hyopneumoniae infection in PAM cells. Moreover, PXR can regulate the mRNA expression levels of IL-6 and IL-8 during M. hyopneumoniae infection of PAM cells. In conclusion, these results suggest that PXR positively regulates CYP3A29 expression during the inflammatory response caused by M. hyopneumoniae infection.

利用chopchop网站对大鼠Inhba基因的第二外显子设计sgRNA位点,通过合成sgRNA寡核苷酸、酶切连接构建到px330载体,再转染px330–Inhba–sgRNA载体到大鼠L6细胞,通过T7E1酶切、T载体克隆测序来验证sgRNA编辑Inhba基因的效率.结果表明:转染pX330–Inbha–sgRNA载体后,Inhba基因的编辑区域的PCR产物能被T7E1酶切割预期条带;T载体克隆测序显示,随机选取的20个单克隆中有5个克隆在预期切割位点附近出现不同长度的碱基缺失,估测编辑效率为25％.可见,本研究设计的sgRNA能够有效利用CRISPR/Cas9系统对Inhba基因进行编辑.

1 背景与意义 近年来,江苏省养猪业在区域发展、产能结构、组织形式等方面均发生了剧烈的变化,生产效率、环境污染和重大疫病防控等问题仍十分严峻.楼房养猪模式是适应新时期养猪业发展需要而兴起的一种新型养殖模式.相比于传统猪舍,楼房猪舍养猪最大优点是节约了土地,提高了土地的利用率.江苏经济发达地区人多地少,工商业发达,猪场建设用地仍面临困境,而采用楼房猪舍养猪就成为了江苏经济发达地区发展养猪业的必然选择.