近年来结直肠癌已成为新发癌症病例发病率第三高和癌症死亡原因第三高的原因，而中国是结直肠癌年均新发病例数和死亡病例数第一的国家 [1-2] 。PLOD1（前胶原赖氨酸2-氧代戊二酸5-双加氧酶1）是编码赖氨酸羟化酶LH1的基因，该基因存在于染色体1p36，包含19个外显子 [3-4] 。赖氨酰羟化酶是一种膜结合的同源二聚体蛋白，定位于内质网的蓄水池，可以催化赖氨酰残基羟基化，所得的羟赖氨酸基团是胶原蛋白中碳水化合物的附着位点，对分子间交联的稳定性至关重要 [5] 。本实验通过二维色谱质谱联用技术以及免疫印迹试验，分析PLOD1在结直肠癌组织及癌旁组织中的表达情况，旨在探究其与结直肠癌发生、发展之间的关系。

Abstract Objective Here, Our study aimed to find the plasma biomarkers related to the diagnosis of multiple sclerosis (MS) through Liquid chromatography Mass Spectrometry (LC/MS) technology. Methods Tandem mass tag (TMT) quantitative LC/MS proteomics method was used to determine the differentially expressed proteins (DEPs) in the plasma samples of 22 MS patients and 22 healthy controls. and the functional annotations of the DEPs were analyzed by GO and IPA. The candidate protein was validated by ELISA. The receiver operating characteristic (ROC) curves were used to determine the predictive potential of the biomarker. Results LC/MS analysis of plasma proteomic identified 88 DEPs among the quantified 375 proteins, of which 39 proteins were up-regulated and 49 proteins were down-regulated. These proteins are involved in immunity/inflammation and Neurological diseases related pathways. A protein panel consisting of Alpha-1-antitrypsin (SERPINA1) and Protein S100-A9 (S100A9) were with an area under the curve (AUC) of 0.991. A MS treatment related protein, DPP4, was validated by ELISA. Conclusion DEPs related to MS can be found in the plasma proteome, which may become biomarkers for MS diagnosis. Our study layed the foundation for the further application plasma proteomics in the diagnosis of MS.

Objective To observe expression changes of platelet membrane glycoproteins(MG) CD61、CD62P in peripheral blood of patients with transient ischemic attack(TIA) and its clinical significance, and to research the effects of aspirin on platelet MG. Methods To mensurate CD61、CD62P expression of 20 cases of TIA patients with flowcytometry(FCM) and monoclonal antibody, including group progressing to infarction and non-progressing group. Whether taking aspirin orally or not, samples were divided into 2 groups. And compare the results between groups and compare with those of 18 cases of healthy volunteers. Results Result The expression of CD61、CD62P in healthy volunteers was lower while that of TIA patients was higher P 0 01; To compare positive percentage of patients progressing to infarction , and that of nonprogressing patients P 0 01;After TIA patients took orally aspirin more than 6 months regularly, the activation rate of platelet and that of patients without aspirin taken orally, P 0 01; To compare that of male TIA patients and female, P 0 05.Conclusion Activation of platelet is an important factor to the onset of TIA, and the higher activation is, the easier thrombosis is formed. CD61、CD62P is able to reflect the activation of platelet so as to forecast the onset of TIA and to estimate its evolvement trend; meanwhile, aspirin plays a remarkable suppressive effect on CD61、CD62P expression.