目的:探讨miR-376c-3p在乳腺癌中的表达水平、生物学功能及分子调控机制.方法:通过实时荧光定量PCR检测乳腺上皮MCF-10A细胞及乳腺癌细胞中miR-376c-3p表达水平;然后,用miR-376c-3p mimic转染乳腺癌MCF-7与MDA-MB-231细胞,用平板克隆实验、划痕实验以及Transwell实验分别检测其增殖与迁移能力.利用Starbase数据库预测miR-376c-3p的下游靶基因,并通过蛋白质印迹实验进行验证.结果:与人乳腺上皮MCF-10A细胞相比,乳腺癌MCF-7和MDA-MB-231细胞中miR-376 c-3p表达水平显著降低(P＜0.01);平板克隆实验显示,过表达miR-376c-3p可以显著抑制乳腺癌细胞增殖(P均＜0.05);Transwell实验和划痕实验表明,miR-376c-3p表达上调可抑制乳腺癌细胞迁移能力;通过生物信息学预测分析,结果显示miR-376 c-3p可以与谷氨酸受体相互作用蛋白1(glutamate receptor interacting protein 1,GRIP1)结合,且蛋白质印迹实验证实过表达miR-376c-3p可以抑制乳腺癌细胞GRIP1表达,同时波形蛋白表达水平明显下调,E-钙黏蛋白表达明显上调.结论:在乳腺癌细胞中,miR-376c-3p表达下调;在体外,过表达miR-376c-3p可显著抑制乳腺癌细胞的增殖和迁移能力,可能通过靶向GRIP1调节乳腺癌细胞上皮间质转化进程.

目的 探讨高迁移率族蛋白-1(HMGB-1)和血管内皮生长因子(VEGF)在直肠癌组织中表达的相关性及其在直肠癌诊断中的应用价值.方法 用免疫组化法检测127例直肠癌和22例直肠黏膜组织中HMGB-1和VEGF的表达情况,分析两者的相关性,并分析其与结肠癌临床病理参数的关系.结果 HMGB-1在直肠癌组织与组织对照组中的阳性率分别为74.8％和13.6％,VEGF分别为42.5％和9.1％,差异均有统计学意义(x2分别为31.1、8.9,P均＜0.01)；两项指标与结肠癌患者的性别、年龄和分化程度无关,但与肿瘤浸润程度和淋巴结转移有关；Spearman相关性分析结果表明,两者之间存在正相关关系(r =0.507,P＜0.05).结论 HMGB-1和VEGF可作为直肠癌侵袭、转移的检测指标.

Objective: To investigate the expression and significance of tumor susceptibility gene 101(TSG101) and EGFR in gastric cancer.Methods: The expression level of TSG101 and EGFR in the tissues of 42 patients with gastric cancer and in the correspondent normal tissues adjacent to the gastric cancer was detected by immunohistochemistry.Results: The positive expression rate of TSG101 in gastric cancer was lower than those in correspondent normal tissues(χ2=12.22,P0.05).The positive expression rate of EGFR in gastric cancer was higher than those in correspondent normal tissues(χ2=10.16,P0.05).Conclusion: The low-express of TSG101 and high-express of EGFR may correlate to the occurrence and development of gastric cancer.