1临床资料患者女,35岁,间断发热、腹痛13年,间断巩膜黄染、尿黄8年,进行性加重三天入院。患者于13年前无明显诱因出现间断发热、腹痛症状,最初未在意,11年前症状加重就诊于上海东方肝胆医院,确诊为"肝内胆管囊性扩张症",随后施于对症保守治疗,好转出院,之后上述症状反复出现,均给

Objective TRPM8 as a clinical biomarker and potential drug target may provide a guidance in accurately judging the malignant potential and prognosis of pancreatic cancer.Downregulating the expression of TRPM8 leads to inhibition of the proliferation of pancreatic cancer cells and this may provide a new target for personalized pancreatic cancer treatment.Methods The pancreatic cancer tissues of 77 patients and the normal pancreas tissues of 30 persons were collected and examined by immunohistochemistry.PCR,gene knockout,Western Blot,CCK8 and patch clamp experiments were performed on pancreatic cancer cell lines BxPC-3,PANC1 and normal pancreatic cell line HPCY-5.Results Immunohistochemistry and Western Blot experiments showed that the TRPM8 channel protein was highly expressed in cancer cells,and the high expression of channel proteins was related to the degree of tumor differentiation (P ＜ 0.05).The detection of the TRPM8 mRNA in pancreatic cancer cell lines BxPC-3 and PANC1 by QPCR showed absolutely high expressions relative to the normal cell line HPCY-5.The results of patch clamp and CCK8 showed that the membrane currents and proliferation of tumor cells were significantly decreased after TRPM8 gene knockout,and the difference is significant (P ＜ 0.05).Conclusions The TRPM8 ion channel was higher in pancreatic cancer cells than that in normal pancreatic cells.Overexpression of TRPM8 was closely related to tumor cell proliferation and invasion ability.

副神经节瘤指位于肾上腺外的嗜铬细胞瘤，是一种罕见的副神经节组织的内分泌肿瘤。原发于肝脏的非功能性副神经节瘤极其罕见，目前国内外仅报道5例［1-3］。吉林大学第二医院2015年4月收治1例。现报道如下。

目的:探讨当前甲状腺微小癌(TMC)的诊断及治疗现状,并为进一步规范外科术式的选择提供临床依据.方法:回顾2013年2月-2015年2月间28例手术和病理均证实为TMC患者的临床资料,对其诊断、手术方式及预后等进行分析.结果:28例TMC患者术前彩超检查均发现病灶,15例彩超下细针穿刺确诊者4例(26.7％),术中冷冻切片确诊者27例(96.4％),术后病理确诊者1例(3.6％).所有患者行手术治疗,包括甲状腺全切除术8例(28.6％)、甲状腺次全切除术7例(25.0％)、甲状腺患侧腺叶+峡部切除术10例(35.7％)、患侧腺叶切除术3例(10.7％).术后均获随访,无复发、转移或死亡者.结论:TMC的检出率有增高趋势,目前的术前明确诊断仍较为困难,外科手术是主要治疗手段;如何提高术前诊断率与规范化手术治疗已成为当务之急.