目的:探讨驱动蛋白超家族蛋白14(KIF14)在肺腺癌(LUAD)中的表达情况及对LUAD细胞生物学功能的影响.方法:通过TCGA、GEO数据库分析KIF14 mRNA在LUAD中表达情况,采用Kaplan-Meier法分析KIF14 表达与患者预后的关系.利用单样本基因集富集分析(ssGSEA)和TIMER数据库探索KIF14表达水平与肿瘤免疫浸润的关系.通过功能富集分析注释KIF14 在肺腺癌中的生物学功能.进一步进行体外实验和免疫组织化学验证.通过实时荧光定量PCR(qPCR)和Western blot检测KIF14 在LUAD细胞系中表达情况;构建KIF14 干扰序列并将其转染入A549 细胞中,将其分为NC组(转染KIF14 阴性对照序列)、siKIF14 组(转染靶向KIF14 的siRNA序列).Western blot检测细胞中KIF14 沉默效果;CCK-8、平板克隆形成实验检测siKIF14 对LUAD细胞增殖能力的影响;TUNEL凋亡实验检测siKIF14 对LUAD细胞凋亡能力的影响;Transwell和划痕实验检测siKIF14 对LUAD细胞侵袭和迁移能力的影响.结果:生物信息数据库分析显示,KIF14 mRNA在LUAD中高表达(P<0.05),且KIF14 mRNA高表达者累积总生存(OS)率较差(P<0.05);功能富集分析表明KIF14 参与细胞分裂、细胞周期、DNA复制等过程.ssGSEA和TIM-ER分析显示KIF14 表达与免疫细胞浸润呈负相关.LUAD 组织及 A549 细胞中 KIF14 均高表达(P<0.05);沉默KIF14 表达能够抑制A549 细胞的增殖、侵袭和迁移能力,诱导细胞凋亡(P<0.05).结论:KIF14 可为肺腺癌诊治过程中的潜在靶点,它可能通过调控细胞增殖、迁移及侵袭等特性影响肺腺癌的发生、发展.

Objective To investigate the correlation between the expression levels of interleukin（IL）-33 and soluble growth stimulating expression gene 2 protein（sST2） in pleural effusion and inflammatory response. Methods A total of 108 patients with pleural effusion admitted to Hebei Provincial Chest Hospital from March 2019 to March 2022 were included in the study retrospectively. Patients with tuberculous pleural effusion（TPE） were included in the TPE group（n=45）, and patients with malignant pleural effusion（MPE） were included in the MPE group（n=60）. In the same period, 50 healthy adults who underwent physical examination in our hospital were selected as the control group（n=50）. The levels of IL-33 and sST2 in pleural fluid and serum IL-33, sST2, IL-6, C-reactive protein（CRP） and tumor necrosis factor-α（TNF-α） were detected. The correlation between pleural effusion, serum IL-33 and sST2 and serum inflammatory factors in patients with TPE and MPE was analyzed by Pearson correlation coefficient. The diagnostic value of pleural effusion and serum IL-33 and sST2 in TPE was analyzed by receiver operating characteristic（ROC） curve. Results The levels of IL-33 and sST2 in pleural effusion were（83.52±21.09）,（1 108.37±236.15） ng/L, respectively, which were higher in TPE group than those in MPE group [（53.16±11.24）,（726.49±151.28） ng/L], the differences were statistically significant（P＜0.05）. The serum levels of IL-33 and sST2 in TPE group and MPE group were higher than those in control group, and TPE group was（74.13±18.49）,（1 454.07±477.19） ng/L, respectively, which was higher than that in MPE group[（58.46±16.52）,（1 228.65±374.64） ng/L], the differences were statistically significant（P＜0.05）. The levels of serum IL-6, CRP and TNF-α in TPE group and MPE group were higher than those in control group, and TPE group was higher than MPE group, the differences were statistically significant（P＜0.05）. Pleural effusion and serum IL-33 and sST2 were positively correlated with serum IL-6, CRP and TNF-α levels in TPE patients（P＜0.05）. The pleural effusion and serum IL-33 and sST2 were positively correlated with serum IL-6, CRP and TNF-α levels in MPE patients（P＜0.05）. ROC analysis showed that IL-33≥69.142 ng/L in pleural effusion, sST2≤943.715 ng/L in pleural effusion, IL-33≥65.172 ng/L in serum, and sST2≥1308.664 ng/L in serum were the best cut-off values for TPE（P＜0.05）. Conclusion Pleural effusion and serum IL-33 and sST2 are closely related to the occurrence and development of TPE and MPE, and are positively correlated with the levels of inflammatory factors such as IL-6, CRP, TNF-α. In clinical practice, TPE and MPE can be identified by detecting pleural effusion and serum IL-33 and sST2.

目的 探讨胸腔积液内白细胞介素27(IL-27)、肿瘤坏死因子α(TNF-α)、糖类抗原15-3(CA15-3)及血管内皮生长因子(VEGF)在结核、肺癌性胸膜炎鉴别中的价值.方法 回顾性选取2018年3月至2021年3月在河北省胸科医院就诊的100例因胸腔积液患者,根据细胞学检验结果分为结核性胸膜炎组62例与肺癌性胸膜炎组38例.比较两组均检测胸腔积液内IL-27、TNF-α、CA15-3及VEGF水平,在受试者工作特征(ROC)曲线的辅助下明确胸腔积液内IL-27、TNF-α、CA15-3及VEGF诊断肺癌性胸膜炎的价值,最后通过一致性分析胸腔积液内IL-27、TNF-α、CA15-3及VEGF联合诊断肺癌性胸膜炎的效能.结果 结核性胸膜炎组患者的IL-27水平为(504.74±113.25)ng/L,显著高于肺癌性胸膜炎组[(504.74±113.25)ng/L],TNF-α、CA15-3及VEGF水平为(47.31±21.48)pg/L、(21.33±9.01)KU/L、(145.31±73.94)pg/L,显著低于肺癌性胸膜炎组[(94.98±57.74)pg/L、(64.71±21.48)KU/L、(419.33±101.46)pg/L],差异均有统计学意义(P<0.05).经ROC分析证实,胸腔积液IL-27、TNF-α、CA15-3及VEGF均能用于肺癌性胸膜炎的诊断,曲线下面积分别为0.935、0.840、0.949、0.934;联合诊断中以胸腔积液IL-27≤402.920 ng/L、TNF-α≥69.188 pg/L、CA15-3≥54.082 KU/L、VEGF≥192.705 pg/L为阳性,任一指标为阳性则判定为联合诊断阳性.经一致性分析证实,联合诊断中阳性32例,敏感度为84.2％,特异度为96.8％,准确率为92.0％,阳性预测值为0.941,阴性预测值为0.910,Kappa=0.827.结论 结核、肺癌性胸膜炎患者胸腔积液内IL-27、TNF-α、CA15-3及VEGF均存在明显差异,且通过ROC分析、一致性分析均证实IL-27、TNF-α、CA15-3及VEGF联合应用于结核、肺癌性胸膜炎的鉴别具有更高的价值.

目的 探讨DNA损伤诱导转录因子4(DDIT4)在肺腺癌细胞中的表达及其对细胞增殖、凋亡的影响和可能机制.方法 实时荧光定量PCR(qRT-PCR)和蛋白质印迹检测DDIT4在肺腺癌细胞系(H1299和A549)及正常肺支气管上皮细胞(BEAS-2B)中表达;慢病毒转染抑制A549细胞中DDIT4表达,分为DDIT4敲低慢病毒(sh-DDIT4,分为sh-DDIT4#1、sh-DDIT4#2和sh-DDIT4#3)组和转染空病毒(NC)组.蛋白质印迹检测细胞中DDIT4沉默效果;CCK-8、平板克隆形成实验检测sh-DDIT4对肺腺癌细胞增殖能力的影响;TUNEL凋亡实验检测sh-DDIT4对肺腺癌细胞凋亡能力的影响;Transwell和划痕实验检测sh-DDIT4对肺腺癌细胞侵袭和迁移能力的影响.结果 qRT-PCR结果显示,DDIT4 mRNA在BEAS-2B、H1299和A549细胞中相对表达水平分别为1.01±0.04、2.19±0.09和3.10±0.09,差异有统计学意义,F=183.00,P<0.001.蛋白质印迹结果显示,DDIT4蛋白在BEAS-2B、H1299和A549中表达水平分别为0.39±0.02、0.98±0.01和1.00±0.02,差异有统计学意义,F=457.00,P<0.001.DDIT4 mRNA在NC、sh-DDIT4#1、sh-DDIT4#2和sh-DDIT4#3组中表达量分别为1.01±0.02、0.58±0.02、0.59±0.02和0.27±0.04,差异有统计学意义,F=150.40,P<0.001;DDIT4蛋白在各组中表达量分别为1.12±0.01、0.61±0.02、0.62±0.02和0.23±0.05,差异有统计学意义,F=145.10,P<0.001.CCK-8增殖实验结果显示,120 h时NC和sh-DDIT4组细胞生长率分别为0.91±0.04和0.57±0.01,差异有统计学意义,t=7.71,P<0.001.克隆形成实验结果显示,NC和sh-DDIT4组细胞克隆形成数量分别为120.70±6.64和10.67±0.88,差异有统计学意义,t=16.42,P<0.001.EdU实验结果显示,NC和sh-DDIT4组细胞的EdU阳性率分别为(64.33±1.76)％和(13.33±1.20)％,差异有统计学意义,t=23.89,P<0.001.细胞凋亡结果显示,NC和sh-DDIT4组细胞凋亡率分别为(22.33±1.45)％和(49.67±0.88)％,差异有统计学意义,t=16.08,P<0.001.细胞划痕实验结果显示,36 h时NC和sh-DDIT4组细胞愈合度分别(84.33±1.86)％和(44.67±2.03)％,差异有统计学意义,t=14.43,P<0.001.Transwell侵袭实验结果显示,NC和sh-DDIT4组穿过小室的细胞数量分别为为(118.00±3.79)和(28.00±1.73)个,差异有统计学意义,t=21.62,P<0.001.结论 DDIT4在肺腺癌细胞中表达上调,沉默DDIT4表达可抑制肺腺癌细胞增殖、迁移及侵袭,诱导细胞凋亡.

目的 探讨持续负压吸引延长带管时间对颈部淋巴结核术后切口的影响.方法 回顾性分析132例颈部淋巴结核术后引流情况按引流方式分为2组,治疗组92例,采用持续负压吸引延长带管时间的引流方式;对照组40例,采用橡皮片或引流管的引流方式.比较2组患者术后引流量、术后住院时间、术后带管时间、切口愈合不良率、术后复发率、切口舒适度等指标.结果 2组术后住院时间分别为(20.63±12.14)d、(17.87±5.51)d,差异无统计学意义(t=1.375,P>0.05).术后引流量分别为(82.80±45.11)ml、(141.99±80.65)ml,差异有统计学意义(t=-5.368,P<0.05);术后带管时间分别为(6.15±2.66)d、(14.95±2.87)d,差异有统计学意义(t=-16.371,P<0.05),术后切口愈合不良率对照组为27.50％,治疗组为9.78％,差异有统计学意义(χ2=6.087,P<0.05).术后复发率对照组为17.50％,治疗组为10.87％,差异无统计学意义(χ2=1.092,P>0.05).2组切口舒适度差异有统计学意义,(Z=-7.911,P<0.05).结论 持续负压吸引延长带管时间可以降低颈部淋巴结核术后切口感染率,是适合颈部淋巴结核手术的引流方式.

目的 探讨功能性颈淋巴结清扫术治疗颈部淋巴结核的疗效.方法 选择2012年6月—2016年5月在河北省胸科医院胸外科收治的颈淋巴结核病例80例,按随机数字表法分为研究组和对照组,每组各40例,研究组采用功能性颈淋巴结清扫术治疗,对照组采用颈淋巴结核病灶清除术治疗,比较两者在术后住院时间、术后引流时间、清除淋巴结数目、淋巴结核阳性率、切口一期愈合率、术后复发率及术后颈肩部功能情况,比较手术前后炎性因子变化.结果 研究组术后住院时间、引流时间均明显低于对照组(t/P=16.015/0.000,t/P=12.360/0.000),清除淋巴结数目明显多于对照组(χ2/P=27.990/0.000),淋巴结结核阳性率研究组低于对照组(χ2/P=14.160/0.000);切口一期愈合率研究组明显优于对照组(χ2/P=6.050/0.014);2组术后15d血清炎性细胞因子(CRP、IL-6、ESR)水平均高于术前,但研究组各指标水平低于对照组(t/P=10.456/0.001,χ2/P=7.255/0.001,χ2/P=4.979/0.001);随访1年研究组术后复发率低于对照组(χ2/P=5.165/0.023),2组颈肩部功能障碍比较差异无统计学意义(P>0.05).结论 功能性颈淋巴结清扫术治疗颈部淋巴结核,术后引流时间、住院时间短,术后复发率低,不影响颈肩部功能.

[Objective] To explore the expression level and clinical significance of poly ADP-ribose polymerase (PARP) protein in esophageal cancer.[Methods] A total of 84 cases with esophageal cancer undergoing the operation in our hospital were selected from May 2014 to February 2015 and divided into the observation group.There were 20 health-checkup examinees with normal esophageal mucosa in the control group.The mRNA and protein expression levels of PARP in both groups were detected by quantitative real-time polymerase chain reaction (QRT-PCR) and immunohistochemistry.The relationship between PARP expression and clinical pathological factors was analyzed.[Results] The mRNA expression level of PARP was significantly higher than that in the control group (P＜0.05).The positive protein expression rate of PARP in esophageal cancer tissues was also significantly higher than that in normal esophageal mucosa (P＜0.05).In patients with esophageal cancer,the expression rate of PARP was correlated with the differentiation degree of esophageal cancer,TMN stage,invasion depth and lymph node metastasis (P＜0.05),while it was unrelated to age,gender and tumor sites (P ＞ 0.05).[Conclusion] The over-expression of PARP may be involved in the occurrence and development of esophageal cancer.The detection of PARP expression may play an auxiliary role in the treatment for esophageal cancer.

Objective To investigate the optimal operation time of empyema necessitates.Methods Clinical data of 72 patients with empyema necessitatis admitted during January 2011 and January 2016 was retrospectively aualyzed.The patients were divided into three groups according to duration between having the first symptom of chest and performing operation.Group A was the duration less than 2 weeks;group B was the duration between 2 to 4 weeks;group C was the duration more than 4 weeks.Operation time,intraoperative bleeding volume,postoperative drainage volume,drainage duration,postoperative hospitalization and incidence rate of postoperative complications were observed in three groups.Results In group A,values of intraoperative bleeding volume,drainage duration and postoperative hospitalization were significantly lower than those in group B and C (P ＜ 0.05).There were no significant differences in operation time,postoperative drainage volume and incidence rate of postoperative complications among three groups (P ＞ 0.05).Conclusion The optimal operation time of empyema necessitates is within two weeks between having the first symptom of chest and performing operation,and patients show quickly postoperative recovery.

Objective To investigate clinical efficacy of focal cleaning with minimal incision of thoracotomy in treatment of empyema necessitates. Methods A total of 67 patients with empyema necessitates during January 2011 and January 2016 were divided into observation group (n=36), who were treated with focal cleaning with minimal incision of thoracotomy, and control group (n=31) who were treated with traditional operation. Efficacy, incision length, intraoper-ative bleeding volume, postoperative pain score, operative duration, postoperative draining volume, laying time of drain-age tube, postoperative hospital stay and incidence rate of complications were observed in two groups. Results All oper-ations were completed successfully in both groups. At the 1st d after surgery, the chest X-ray showed that the abscess cav-ity disappeared, and the lung recruitment was good. Values of incision length, intraoperative bleeding volume, postopera-tive pain score, postoperative draining volume, laying time of drainage tube and hospital stay in observation group were lower than those in control group (P<0. 05). There were no significant differences in operative duration and incidence rate of complications in two groups (P>0. 05). Conclusion On the basis of enough understanding indications and per-forming preoperative evaluation, focal cleaning with minimal incision of thoracotomy in treatment of empyema necessitates has the advantages such as shorter incision length, less bleeding volume and postoperative draining volume, lower postop-erative pain score and shorter postoperative hospital stay.

Objective To investigate the role of electric knife sharp separation in decortication treatment for patients with chronic tuberculous empyema .Methods From August 2012 to February 2015, 92 cases of stage III of chronic tubercu-lous empyema were randomly divided into two groups in Hebei Chest Hospital .The experimental group had 46 cases and con-trol group had 46 cases.In experimental group , patients were treated with decortication by using electric knife sharply separa-ted the fiber board, in control group, traditional decortication was performed .The operation time, intraoperative blood loss, drainage quantity after 24 h postoperatively , intubation time , the amount of blood transfusion , and the incidence of postopera-tive hydro-pneumothorax , the incidence of postoperative bleeding and the incidence of liquefaction of the subcutaneous fat and wound infection were compared between the two groups .Results The experimental group and the control group ’ s average op-eration time were (253.59 ±71.64) min and (255.11 ±68.02) min,there was no statistically difference between the two groups( P >0.05).The intraoperative blood loss, drainage quantity after 24h postoperatively, intubation time,the amount of blood transfusion of experimental group [(163.37 ±104.03) ml, (285.37 ±84.57) ml,(7.91 ±2.79 ) d, (115.22 ± 168.61)ml] were significantly decreased compared to control [(611.30 ±184.25)ml, (502.17 ±173.76)ml, (10.24 ± 3.75) d, (602.17 ±268.73)ml, t =-14.358,-7.609,-3.377,-10.066, P <0.01],incidence of postoperative hydro-pneumothorax and incidence of postoperative bleeding of control group (17.39%, 13.04%) was higher than experimental group (P <0.05).The incidence of liquefaction of the subcutaneous fat and wound infection were 8.70% and 13.04%(P <0.05).Conclusion Electric knife sharp separation for decortication can reduce intraoperative blood loss , postoperative 24 h after drainage and shorten the intubation time , reduce the amount of blood products transfusion , reduce the incidence rate of intrathoracic bleeding and pneumothorax after operation , has the value of popularization and application .

Objective To explore the effect of continuous negative pressure drainage in the postoperative recovery of tuberculous empyema necessitatis.Methods Sixty-eight patients with tuberculous empyema necessitatis were researched,34 patients treated by continuous negative pressure drainage were in the observation group,and the other 34 patients treated by passive drainage were in the control group.The amount of post-operation drainage,drainage duration,postoperative visual analogue scale(VAS) score,postoperative hospital stay,lung recruitment,and the rate of poor incision healing were compared between the two groups.Results The drainage duration and the postoperative hospital stay of the observation group was shorter than the control group,the difference was statistically significant(P<0.05).The amount of post-operation drainage and the postoperative VAS score of the two groups were not significantly different(P>0.05).The rate of poor incision healing of the observation group was lower than the control group,the difference was statistically significant(P<0.05).Conclusion On the basis of strict indications,continuous negative pressure drainage showed many positive effects in postoperative recovery of tuberculous empyema necessitates.

Objective To explore the efficacy of autologous pleural patch in surgical operation on the patient with chronic tuberculous empyema.Methods Autologous pleura patches were used to repair the pulmonary wound in the surgical operations for 7 patients with chronic tuberculous empyema,who received surgical management in Hebei Provincial Chest Hospital from August 2012 to November 2014.The leak time of the patient and the time of the chest tube were recorded,the pulmonary re-examination was observed by chest X-ray,and the follow-up results were recorded.Results The time of persistent pulmonary air leak of 6 patients was not more than 10 hours after operation,1 patient was not more than 24 hours.Review of X-ray chest showed that good for lung,4-7 d after operation removal of thoracic dosed drainage tube,there was no death and no chest infection in 7 patients and review of CT chest showed no significant residual cavity.After follow-up for 6-24 months 7 patients had satisfactory efficacy,there was no pneumothorax and thoracic infection,no residual cavily.Conclusion Autologous pleura can be used to patch the pulmonary wound in the surgical operation on the patient with chronic tuberculous empyema.

Objective To explore the effect of local anesthesia monoporate thorascopic surgery in treatment of traumatogenic hemopneumothorax. Methods A total of 180 patients with traumatogenic hemopneumothorax during June 2009 and February 2014 were divided into two groups (n = 90 each) with block randomization method. The observation group was treated with local anesthesia monoporate thoracic surgery, and the control group received traditional thoracic closed drainage. In the two groups, the postoperative drainage volume and the keeping tube time of thoracic closed drain-age were observed, and the time from admission to transference exploratory thoracotomy, complications and the average hospital stay were also recorded. Results All the patients were cured and discharged. The postoperative drainage vol-ume, the keeping tube time of thoracic closed drainage, the time from admission to transference exploratory thoracotomy, complications and the average hospital stay in observation group were superior to those in control group (P < 0. 05). The difference in number of transference exploratory thoracotomy was not statistically significant (P > 0. 05). Conclusion The local anesthesia monoporate thorascopic surgery in treatment of traumatogenic hemopneumothorax may reduce bleed-ing volume, incidence rate of complications, the keeping tube time of closed drainage of pleural cavity and average hospi-tal stay. It has better curative effect than the traditional therapy of thoracic closed drainage.

Objective To investigate the expression and clinical significance of CXCR4,HPA and MMP-2 in esophageal squamous cell cancer( ESCC). Methods One hundred and twenty specimens of ESCC and 10 specimens of adjacent ESCC normal tissue were stained by immunohistochemisty,then the expression levels of CXCR4,HPA,MMP-2 were detected,and the correlation among the three factors was analyzed. Results The positive expression rates of CXCR4,HPA,MMP-2 in ESCC tissues were 74. 17%,58. 33%,67. 50%,respectively,which were significantly higher than those in adjacent ESCC normal tissues( P 0. 05). The expression levels of CXCR4,HPA,MMP-2 were correlated to the infiltration depth,size,lymph node metastasis,TNM staging of ESCC( P 0. 05). The expression of CXCR4 was positively related with that of HPA /MMP-2( r = 0. 267 / r = 0. 206,P 0. 05),however,the expression of HPA was not correlated to that of MMP-2( P 0. 05). Conclusion CXCR4 plays an important role during the progress of ESCC,which may increase activity of HPA /MMP-2 through certain mechanisms,as a result,which may promote the invasion and metastasis of ESCC.

Objective To find a new therapy to treat the tuberculous pleurisy by thoracoscope.Methods 90 cases of patients with tuberculous pleurisy were randomly selected for this study.They were treated with the same chemotherapy.The experimental group accepted the extra therapy with thoracoscope besides thoracocentesis,while the control group accepted the conventional treatment of the thoracocentesis.Clinical symptoms,pleural effusion,thickness of pleura,hospital stay and the rate forming empyema after 3 months were recorded.Results The experimental group got a better results than the control group,the difference has a statistical significance.The therapy of tuberculous pleurisy by thoracoscope is an effective method.Conclution Our therapy is worthy for extending in clinic because it's safer,more convenient and could get pathological diagnosis at the same time.

Objective: To investigate the expression and clinical significance of CXCR4,VEGF in NSCLC.Methods: CXCR4-VEGF expression was evaluated by immunohistochemistry in 86 selected cases of primary NSCLC and 13 cases in chest wall NSCLC metastases and 20 samples of normal lung tissues.The relationship of the expression and clinical pathological features were analyzed.Results: The positive rate of CXCR4 in NSCLC was significantly higher than in normal lung tissue,but it was significantly lower than in chest wall NSCLC metastases.The positive rate of VEGF in NSCLC tissue was significantly higher than in normal lung tissue.The positive rate of VEGF in normal lung tissue was significantly higher than in chest wall NSCLC metastases.There was significant positive correlation between the expression of CXCR4 and lymph node metastasis and TNM stage(P < 0.05).There was significant positive correlation between the expressions of VEGF and lymph node metastasis,TNM stage(P < 0.05).The expression of CXCR4 had positive correlation with VEGF in NSCLC(P < 0.05).Conclusion: CXCR4-VEGF pathway plays a role in NSCLC metastases and may be new prognostic marker in NSCLC.Moreover,these results suggest that targeted inhibition of CXCR4 could be introduced to control metastasis development in NSCLC.

Objective To investigate the expression and clinical significance of CXCR4,MMP-2in non-small cell lung cancer.Methods Expressions of CXCR4and MMP-2were evaluated by immunohistochemical staining in 60surgically resected specimens of non-small cell lung cancer,30cases of regional metastasized lymph nodes and 20samples of normal lung tissues.The relationship between the two protein was analyzed.Results CXCR4protein was detected in 68.33% cases of primary NSCLC,in 93.33% cases of regional metastasized lymph nodes,and in 20% cases of normal lung tissues.MMP-2expression in 58.33%in NSCLC,in 43.33%in metastasized lymph nodes and in 15%cases of normal lung tissues.Expression of CXCR4was associated with lymph node metastasis and TNM stage(P0.05)MMP-2 expression was significantly associated with lymph node metastasis,the degree of?histological differentiation and TNM stage(P0.05).CXCR4expression was positively correlated with MMP-2expression(P0.05).Conclusion CXCR4 may play an important role during the progression of NSCLC and there might be some regulatory mechanism existing between CXCR4and MMP-2.