Objectives To investigate the protective effect and mechanism of a gonadotropin-releasing hormone antagonist(GnRH-ant) against an ovarian function injury induced by pelvic radiotherapy in a rat model.Method 1.Ten female Wistar rats were randomly assigned to gonadotropin-releasing hormone agonist(GnRH-a) and GnRH-ant groups using the random number table method.The rats were subcutaneously injected with goserelin (0.25 mg once)or cetrorelix (5 μg/day for 10 days).Changes in luteinizing hormone(LH) and estradiol(E2) in each group were dynamically observed.2.Forty female Wistar rats were randomly divided into four groups(control,GnRH-ant,R,and GnRH-ant +R) and then given corresponding treatments.Ovarian wet weight,levels of serum LH,E2,and AMH,and the number of follicles at every stage were compaed between groups through analysis of variance and independent sample t-test.Results 1.In the GnRH-a group,LH and E2 levels increased initially and then gradually decreased,reaching a low value in approximately 10 d.In the GnRH-ant group,LH and E2 levels decreased rapidly,reaching the minimum value in 4 d without flare-up effect.2.After pelvic radiotherapy,the ovarian wet weight in the GnRH-ant+R group was significantly higher[(58.3±9.1) mg vs.(37.8±7.1) mg,t=5.61,P=0.000]than that in group R.In the GnRH-ant+R group,the levels of E2[(57.49±13.45) pg/mL vs.(16.64±6.54) pg/mL,t=8.64,P=0.000] and AMH[(5.47±1.32) mIU/mL vs.(2.23±0.72) mIU/mL,t=6.81,P=0.000] were significantly higher than those in group R.The FSH level in GnRH-ant+R group was significantly lower[(27.74±7.75) mIU/mLvs.(8.35 ±1.43) mIU/mL,t=7.75,P=0.000] than that in group R.The number of primordial and primary follicles in group GnRH-ant+R was significantly higher(46.2±12.3 vs.27.6±5.1,t=4.42,P=0.000) than that in group R.Conclusions GnRH-ant can rapidly induce ovarian inhibition without flare-up effect.Subcutaneous injection of GnRH-ant before pelvic radiotherapy can inhibit the ovary and stop the follicles in the primary and primordial follicle stages,thus reducing the damage induced by radiotherapy.

Objective To investigate the protective effect of gonadotropin-releasing hormone agonist (GnRHa) on ovarian function in rats during pelvic irradiation and its possible mechanism.Methods 1.Five female Fischer-344 rats were subcutaneously injected with 0.25 mg of GnRHa.Changes in serum follicle-stimulating hormone(FSH),estradiol(E2),and anti-mullerian hormone(AMH) levels were observed continually.2.Forty female rats were randomly divided into four groups(Control,GnRHa,R,and GnRHa+ R) and given their corresponding treatments.At 20 days after pelvic irradiation,the weight;ovarian wet weight;serum FSH,E2,and AMH levels;and follicular numbers of the four groups were compared using one-way ANOVA and independent-sample t-test.The apoptotic index and microvessel density(MVD) in the ovarian tissue of each group were also compared.Results 1.Treatment with GnRHa inhibited ovarian function.Under this treatment,serum FSH and E2 declined,reached the minimum values in approximately 15 days,and then normalized in approximately 20 days.2.After pelvic radiotherapy,the GnRHa+R and R groups showed different degrees of ovarian function damage compared with the control group,but the damage to the GnRHa+R group was less severe compared with that to the other groups.The GnRHa+R group showed higher E2(t =12.79,P ＜ 0.01),lower FSH(t =4.65,P ＜ 0.01),and relatively higher AMH (t =5.65,P＜ 0.01) compared with the R group.Follicular classification revealed significantly more primordial and primary follicles in the GnRHa+R group than in the R group(t=7.70,P＜0.01).Tunnel and CD31 staining showed that the apoptotie index and MVD were significantly higher in the R group than in the GnRHa+R group(t =8.20 and 9.83,both P ＜ 0.05).Conclusions Administration of GnRHa before radiotherapy can significantly decrease radiation damage to ovarian function in rats.GnRHa exerts its protective effect against ovarian functional impairment by inhibiting follicular development in primordial and primary follicles.It decreases the blood supply and oxygen of ovarian tissue,thereby reducing the radiation sensitivity of the ovary.

目的:研究芪红脑苏汤活血化瘀作用对局灶性脑缺血再灌注损伤大鼠的脑保护作用,并探讨其作用机制.方法:以线栓法复制局灶性脑缺血再灌注损伤大鼠模型,观察芪红脑苏汤对局灶性脑缺血再灌注损伤大鼠脑组织病理形态改变及脑组织中TNF-α、ICAM-1含量的影响.结果:与模型组比较,芪红脑苏汤31.4g/kg、15.7g/kg、7.9g/kg剂量组均能降低大鼠神经功能缺失评分及其病理评分分值;芪红脑苏汤31.4g/kg剂量组能降低缺血部位TNF-α表达,31.4g/kg、15.7g/kg剂量组能降低缺血部位ICAM-1的表达;芪红脑苏汤31.4g/kg、15.7g/kg、7.9g/kg剂量组均能降低缺血部位TNF-α含量,15.7g/kg、7.9g/kg剂量组能降低缺血部位ICAM-1的含量.结论:芪红脑苏汤的活血化瘀作用对局灶性脑缺血再灌注大鼠的脑损伤具有较好的保护作用,其作用机制可能与降低脑内TNF-α、ICAM-1含量有关.

目的:观察芪红脑苏汤治疗对小鼠体外凝血时间、血瘀证大鼠血小板聚集率、凝血因子及血液流变学和体内血栓形成的影响.方法:50只KM小鼠随机分为空白对照组、法华林钠0.5 mg/kg剂量组、芪红脑苏汤15.7、31.4、62.8g/kg剂量组,连续灌胃给药7天,观察各组小鼠体外凝血时间的变化.60只SD大鼠随机分为空白对照组、模型对照组、尼莫地平8mg/kg剂量组、芪红脑苏汤7.9、15.7、31.4g/kg剂量组,连续灌胃14天后,皮下注射盐酸肾上腺素联合冰水内游泳复制血瘀证模型,观察芪红脑苏汤对血小板聚集率、凝血功能及血液流变学指标的影响.50只KM小鼠,随机分为空白对照组、尼莫地平16mg/kg剂量组、芪红脑苏汤15.7、31.4、62.8g/kg剂量组,连续灌胃给药7天后,除空白对照组外,各组小鼠尾静脉注射血栓诱导剂,对照组注射等体积生理盐水,观察各组小鼠的偏瘫数,偏瘫的恢复数及死亡数,计算偏瘫率与恢复率.结果:芪红脑苏汤15.7g/kg、62.8g/kg均能延长小鼠凝血时间;芪红脑苏汤7.9g/kg、31.4g/kg均能抑制急性血瘀证模型大鼠血小板聚集,芪红脑苏汤7.9g/kg、15.7、31.4g/kg均能延长PT和APTT,7.9、15.7、31.4g/kg能降低大鼠全血粘度,31.4 g/kg能降低血浆黏度、红细胞压积、纤维蛋白;芪红脑苏汤15.7 g/kg、31.4 g/kg、62.8 g/kg均能降低"胶原蛋白-肾上腺素"混合诱导剂致体内血栓偏瘫小鼠偏瘫率和死亡率.结论:芪红脑苏汤具有活血化瘀作用.

Objective:To establish a quality control model for Bio-Psycho-Social integrative intervention for relapse prevention based in community,and explore the effect of this model on relapse intervention.Methods:After the quality control model established by experts consultation,we applied this model in 7 communities of ShiZhong administrative area,Leshan,SiChuan province,and other 27 communities as control,the integrative intervention was used in all these areas.The effects were compared between the two groups.Results:In experiment communities,64.0% of the objects relapsed in half a year and 82.0% relapsed in a year,with 84.0% and 93.0% as control.In experiment communities,52.0% of patients took part in methadone maintenance treatment,which is higher than control communities(25.5%);Both the rate of involving in compulsive isolated drug rehabilitation and death rate were lower than control group.What's more,some patients of experimental group begin to earn their living by their own efforts successfully.Conclusion:The Bio-Psycho-Social integrative intervention model for relapse prevention is effective and carrying out a scientific,reasonable and easy-operational quality control can improve this effect.

目的了解乐山市艾滋病感染者/药物依赖患者目前的生存环境,为确定综合干预防复吸的干预重点提供参考依据。方法采用问卷调查、座谈会等方法进行调查,并对调查数据进行分析评估。结果艾滋病感染者/药物依赖患者普遍感受到来自社会生存环境的严重歧视,住房问题、医疗保健问题、自助自救是该群体目前最需要解决的问题。结论各级政府相关职能部门应加强社区宣传教育,进一步消除歧视,加大对艾滋病感染者/药物依赖患者的关怀支持;政府应就针对目标人群的廉租房补贴、医疗救助、低保机制及自助自救等方面进行更深入的政策开发。