[基金项目] 国家自然科学基金资助项目(No. 81201607);河北杰出青年基金资助项目(No. H2014206320);河北人才培养项目资助 (No. A201401040)。Project supported by the National Natural Science Foundation of China (No.81201607), the Outstanding Youth Foundation of Hebei Province (No. H2014206320), and the Foundation for High Level Talents of Hebei Province (No. A201401040) [作者简介] 李欢(1993-),女,硕士生,主要从事肿瘤免疫治疗的临床与基础研究,E-mail: vandol_lh@163.com [通信作者] 刘丽华(LIU Lihua,corresponding author),博士,教授,主任医师,博士生导师,主要从事肿瘤免疫与生物治疗,E-mail: lihualiu567@hotmail.com 桥接整合因子1通过 c-MYC途径抑制非小细胞肺癌A549细胞中PD-L1 的表达

Objective To observe the pathophysiological change of acute lung injury in rats with severe acute pancreatitis(SAP),and to explore its pathogenesis mechanism .Methods The 240 healthy male SD(Sprague-Dawley) rats from January 2008 to 2009 January were enrolled in the study , these rats were randomly divided into 8 groups according to random digits table,7 observation groups and control group ,the observation groups included 7 different time points of SAP ,ie. 1h group,3h group,6h group,9h group,12h group,18h group,24h group and control group ,with 30 rats in each group.The changes of serum amylase , the ratio of lung tissue wet/dry weight , lung myeloperoxidase ( MPO ) activity and the lungs permeability index were detected and compared among the 8 groups.Results The lung injury score,the ratio of lung tissue wet/dry weight and lung MPO levels in observation groups were gradually increased ,as compared with those in control group , moreover,which in 7 time points were significantly higher than those in control group ( P <0.05).The lung permeability index in observation groups was gradually decreased ,as compared with that in control group ( P <0.05).The MPO levels in lung tissue were positively correlated to lung injury score ( r =0.946, P <0.01),and the ratio of lung tissue wet/dry weight was positively correlated to lung injury score ( r =0.879, P <0.01),however,lungs permeability index was negatively correlated to lung injury score( r =0.859, P <00.1 ).Conclu sion The lung injury degree of rats with SAP is gradually increased with prolongation of course of disease ,moreover ,the MPO levels in lung tissue are continually increased .