10 lesioned lung tissues from typical swine mycoplasmosis were grinded,cleaned,and subjected to isolation,and a mycoplasma strain was obtained.The mycoplasma was proved to be Mycoplasma hyopneumoniae (Mhp)by culture character,serological identification,biochemical identification,PCR test and sequencing.The purefied isolated strain was named Mhp strain S.Afterwards,Homology comparison was performed on genetic sequence and amino acid sequence of P46 and P97 R1 region with the available sequence of other strains,the results show that the amino acid sequence of P46 gene is more than 99% homology with other strains,the number of P97 R1 region amino acid repeat sequence of the Mhp strain S is 11,different from other strains.The immunogenicity test results showed that the isolation strain had good immunogenicity.

选用猪、兔、鸡、小鼠和豚鼠5种试验动物分别制备猪肺炎支原体高免阳性血清,并测定了其对猪肺炎支原体的代谢抑制效价.再从兔高免血清中纯化特异性抗体,比较抗体纯化前后的代谢抑制效价是否发生变化.结果表明,5种试验动物高免血清对猪肺炎支原体的代谢抑制价分别为0、104、103、105、102,纯化后的抗体仍可抑制猪肺炎支原体的生长,代谢抑制价没有明显变化.由此可见,不同动物来源的高免血清对猪肺炎支原体的代谢抑制价不同,代谢抑制作用的主要作用物质为特异性抗体;首次证明病原靶动物——猪的高免抗血清对猪肺炎支原体没有代谢抑制作用,其他支原体是否也表现为对靶动物(或人)抗血清的耐受性还有待进一步研究.