Objective:To extract stem cells through normal urine and to investigate the effects of conditioned medium(CM) from human urine derived stem cells(hUSCs)on podocyte lesion induced by high glucose. Methods:hUSCs were isolated from void-ed urine of healthy men. After identification and proliferation,hUSCs - CM was prepared. According to the different culture condi-tions,human podocytes were divided into normal glucose group,high permeability group,high glucose group(HG),and HG +hUSCs - CM(1 × ,5 × ,10 × )group. Real - time PCR was applied to detect the expression levels of synaptopodin,and human podocytes apoptosis in each group were examined by flow cytometry after cultured for 24 hours. Results:Compared with incubated in NG,the expression of synaptopodin mRNA in HG was found to decrease(vs. NG,P < 0. 05). HUSCs - CM increased the expression of podocytic synaptopodin mRNA(vs. HG,P < 0. 05). Early Podocytes apoptosis rate was mildly higher in HG group than in NG group(P < 0. 05),hUSCs - CM reduced the early podocytes apoptosis in a dose - dependent manner(vs. HG,P < 0. 05). Conclu-sion:hUSCs - CM prevents podocytic apoptosis and injury induced by HG,and may be a new therapeutic method to ameliorate the progress of DKD.