挑选自然感染圆环病毒Ⅱ型的病猪56头,随机分为高、中、低剂量中药配合干扰素治疗组、治疗对照组和空白对照组.各组于处理后1周统计疗效,结果表明:中药高、中剂量配合干扰素组的治愈率和总有效率显著高于空白对照组和低剂量组.表明中草药制剂配合干扰素对猪圆环病毒Ⅱ型感染疗效较好.

Chinese herbal extract has been confirmed to be effective on precautions of diseases induced by porcine reproductive and respiratory syndrome virus (PRRSV),porcine circus virus (PCV-2),etc.The target of this test was to determine the effect of Chinese herbal extract on anti -infectious potential of Marc -145 cell in PRRSV challenge,within the consistency scope of cell safety.Polygonum Cuspidatum Sieb.et Zucc,Nv -zhenzi, Herba Polygoni Perfoliati and other 7 kinds of Chinese herbs were chosen for the aqueous extraction.Then the stock solution were concentrated by rotary evaporation to 25 mg/mL and diluted to 10 levels:2 500,1 250, 625.0,312.5,156.25,78.13,39.06,19.53,9.77,4.88 μg/mL.The injection method followed by:1)injecting 0.1 mL dose into the Marc-145 cell culture and hatching 2 h,then injecting 0.1 mL PRRSV(100 TCID50),4 replicates for each level;2)injecting 0.1 mL PRRSV and hatching 2 h,then injecting 0.1 mL dose, 4 replicates for each level;3)injecting 0.1 mL dose and 0.1 mL PRRSV at the same time,6 replicates for each level, and there were both virus and cell control with each treatment. Results showed that 19.53~2 500 μg/mL dose may work significantly and 39.06 μg/mL do the best with method 1;156.25~2 500 μg/mL dose may work remarkably and 1 250 μg/mL do the best with method 2;with method 3,19.53~2 500 μg/mL may work efficiently and 2 500 μg/mL do the best.This could be one mechanism of action of Chinese herbal extract to prevent PRRSV infection.

犬瘟热(Canine distemper,CD)是由犬瘟热病毒(Canine distemper virus,CDV)引起的一种急性、热性、败血陛、高度接触性传染病,致死率很高.

体外抗病毒试验结果显示多肽药物Alloferon 1对犬瘟热病毒具有较好的抑制作用,在一定浓度范围内药物浓度越高,对病毒的抑制效果越好。利用脑内注射犬瘟热病毒建立犬瘟热病小鼠模型,然后用Alloferon 1对犬瘟热病毒感染的小鼠进行体内保护性试验。体内试验结果表明,多肽药物Alloferon 1对犬瘟热病毒感染的小鼠有明显的保护作用,可望成为犬瘟热病防治的一种新型药物。