目的 了解沈阳地区不同年龄段患者中人巨细胞病毒?(HCMV)?的感染状况.方法 选择疑似HCMV感染患者123616例,采用荧光定量PCR技术检测HCMV?DNA,化学发光免疫分析法检测血清HCMV?IgM和IgG抗体,收集患者临床诊断信息.结果 HCMV?DNA、IgM抗体、IgG抗体阳性率分别为12.48％、2.34％、95.58％.>28?d～1岁组患儿HCMV?DNA阳性率?(31.52％)、IgM抗体阳性率?(9.86％)?最高.不同体液HCMV?DNA阳性率不同,尿HCMV?DNA阳性率?(14.61％)?大于血清?(2.36％)?和肺泡灌洗液?(2.17％).HCMV?DNA阳性率与IgM抗体阳性率呈正相关,HCMV?DNA阳性患者中,HCMV?IgM抗体阳性者病毒载量高于IgM抗体阴性者.HCMV感染的临床诊断包括肺炎、神经系统损害、肝炎、血液病等.结论 不同年龄段患者的HCMV感染状况存在差异.婴儿是HCMV易感人群,HCMV感染后主要累及肺脏、肝脏、神经系统等.多种体液样本HCMV?DNA检测以及HCMV?DNA与IgM抗体联合检测对HCMV检测和诊治有重要的意义.
目的 探讨沈阳地区手足口病病原体分布及流行特征,为手足口病的防控和临床诊治提供参考依据.方法 采用描述性流行病学方法,对2014—2018年中国医科大学附属盛京医院就诊的手足口病患儿病例信息及病原学监测数据进行分析.结果 共接诊临床诊断手足口病患儿25571例,17263例(67.51％)肠道病毒检测阳性,其中男患儿阳性检出率为69.72％(10305/14780),女患儿阳性检出率为64.48％(6958/10791),男女患儿阳性检出率比较,差异有统计学意义(χ2=78.167,P<0.001).手足口病肠道病毒检测阳性患儿中,<6岁者占92.59％,其中3～岁年龄组占比最高(24.53％).手足口病全年均有发病,但季节分布明显,呈单峰状分布,每年7、8月份最高,占比为29.94％～52.30％.手足口病分离病原体EV-71占12.71％,CV-A16占24.38％,其他肠道病毒占62.91％.结论 除EV-71和CV-A16外沈阳地区手足口病患儿感染其他肠道病毒所占比例较高,每年7、8月份为手足口病高发期,以<6岁的儿童发病为主,男孩发病居多.
Objective To investigate the distribution of human papillomavirus (HPV) infection characteristics and genotypes in Shenyang area of Liaoning province.Methods HPV genes were detected in cervical exfoliated cells from 55,548 patients by amplification and diversion hybridization.Results A total of 9,566 patients were positive for HPV infection with a positive rate of 17.22％.Additionally,the positive rate of high risk HPV infection was 14.57％ and the positive rate of single genotype HPV infection was 13.63％.Totally,12,360 HPV viruses were detected.Among them,10,879 HPV viruses were classified into high risk genotypes (10,879 out of 12,360,88.02％).The genotypes in women with ages less than 20 were 16/11/6/51/58/52 genotypes;the susceptible HPV genotypes in other women were 16/58/52/53/39/51/81 genotypes.Conclusions HPV infections in Shenyang are mainly infections with high risk viruses and single infection.The infection rate and genotype distribution of HPV are different in different age groups.More suitable HPV vaccine prophylaxis can be taken according to the epidemic characteristics of HPV in this area.
