Objective To observe the expression of acid -sensing ion channels (ASICs) in renal tissues from patients with Henoch -Sch?nlein purpura nephritis (HSPN) and to explore the action and mechanism of ASICs in the inflammatory injury of renal tissues. Methods Immunohistochemical methods were used to investigate the expression and localization of ASICs in renal tissue from patients with HSPN and patients with dropsical nephritis.The expressions of ASICs were semiquantitatively assessed by the percentage of posi-tive area of ASIC1a, ASIC2a, and ASIC3.The correlation between expression of ASICs and quantity of 24 -hour urinary protein was analyzed.According to different expressions of α1 -MG and β2 -MG in the test of urine trace protein, patients with HSPN were divid-ed into two groups.The expressions of ASICs in renal tubules of those two groups were assessed respectively.Results Immunohisto-chemical analysis revealed that higher expression of ASIC1a, ASIC2a and ASIC3 was noted in renal tubules from patients with HSPN. The expression of ASIC3 in patients with HSPN was positively correlated with quantity of 24 -hour urinary protein.Compared with the group with normal expressions of α1 -MG and β2 -MG, the expression of ASIC3 was significantly increased in the group with abnormal expressions of α1 -MG and β2 -MG (P <0.01) and higher expression of ASIC2a was noted in the group with abnormal expressions ofα1 -MG (P <0.01).Conclusions This study suggested an excitive role of ASICs in the pathogenesis of the injury of renal tubules in patients with HSPN.The expression of ASICs may be related to the production of proteinuria, especially microalbuminuria.

目的研究新合成的全反式维甲酸（ATRA）衍生物（4-氨基-2-三氟甲基苯基维甲酸酯）对人肝癌细胞株HepG2体外增殖、凋亡及分化情况的影响。方法3种浓度（1、5、10μmol/L）的ATRA衍生物分别处理HepG2细胞,1、2、3、7d后用MTT法检测4-氨基-2-三氟甲基苯基维甲酸酯对HepG2细胞增殖的影响;酶动力学法检测γ-谷氨酰转肽酶（γ-GT）比活性和甲胎蛋白（AFP）分泌量变化;流式细胞术分析细胞周期及凋亡情况;Western blot检测HepG2细胞中凋亡相关蛋白的表达量。结果10μmol/L的4-氨基-2-三氟甲基苯基维甲酸酯可明显诱导HepG2细胞凋亡,凋亡率达到49%,并且使代表肝细胞恶变的γ-GT比活性、AFP分泌量明显下降,Western blot显示4-氨基-2-三氟甲基苯基维甲酸酯显著抑制Bcl-2、Bcl-xS/L和p-Erk表达。结论4-氨基-2-三氟甲基苯基维甲酸酯是肝癌细胞HepG2有效的分化诱导剂,并明显促进肝癌细胞凋亡。