目的 观察田基黄水提物对肝癌细胞HepG2凋亡的影响并探讨其作用机制.方法 人肝癌细胞HepG2经培养后分为4组,其中,对照(A)组不加入田基黄水提物处理,B、C、D组分别加入浓度为0.5、1.0、2.0 mg/ml的田基黄水提物处理24 h.通过倒置显微镜观察各组细胞形态变化,流式细胞术检测各组肝癌细胞早期凋亡情况及线粒体膜电位的改变;利用逆转录聚合酶链反应(RT-PCR)检测各组线粒体上相关基因B细胞淋巴瘤(Bcl)-2和Bcl-2相关X蛋白(Bax)的mRNA表达.结果 B、C、D组可见人肝癌细胞HepG2出现明显的凋亡形态学变化;流式细胞术检测发现,B、C、D组细胞膜内侧的磷脂酰丝氨酸(PS)外翻,提示早期细胞凋亡.与A组相比,B、C、D组人肝癌细胞HepG2凋亡率明显增加,且呈剂量依赖性(P<0.05);线粒体膜电位检测提示,与A组相比,B、C、D组细胞线粒体膜电位改变率明显升高,并呈剂量依赖性(P<0.05);与A组相比,B、C、D组还明显上调了Bax mRNA表达水平,显著下调Bcl-2 mRNA表达水平(P<0.05).结论 田基黄水提物可通过线粒体凋亡通路来诱导肝癌细胞凋亡.

Objective Difference of protein expressions of highly griseofulvin-producing Penicillum griseofulvum F208 during fermentation was confirmed by proteomics techniques in order to explore the key enzymes in the course of biosynthesis of griseofulvin. Method The techniques of two-dimensional gel electrophoresis and mass spectrometry were used to analyze the differential expression of proteins from P.griseofulvum F208 during the course of fermentation. Result The expressed proteins had been found to differ from each other between the sample cultured at 72 h and 192 h(which was in antibiotic production phase).The two special protein spots increased in abundance in sample cultured at 192 h were successfully identified as serine hydroxymethyl transferase and S-adenosylmethionine synthetase respectively. Conclusion A set of method of two-dimensional gel electrophoresis for total proteins from Penicillium griseofulvum mycelia were established.And the two special protein spots identified might be related with biosynthesis of griseofulvin.

Objective To explore the inhibitory effect of different extract parts from Hypericum japonicum on human hepatoma cell line HepG2.Methods Hypericum japonicum was suspended in 95% ethanol,and then extracted successively with petroleum ether,methylene chloride,ethyl acetate and n-butanol.Human hepatoma cell line HepG2 were cultured in vitro and treated with different extract parts from Hypericum japonicum,then cellular morphology was observed via phase-contrast microscopy,and the cell viability was determined by MTT assay.Results The four extract parts from Hypericum japonicum with a certain concentration inhibited the growth of human hepatoma cell line HepG2 in a dose dependent manner.Conclusion The four extract parts from Hypericum japonicum can inhibit the growth of human hepatoma cell line HepG2 effectively.