OBJECTIVE:To investigate the effect of Fushengong Decoction on the expression of nephrin mRNA in renal tissue of rats with chronic renal failure (CRF).METHODS:Fifty five male SD rats were randomly divided into five groups: control group, CRF model group, and low, medium and high Fushengong Decoction dose groups. Rats in control group were fed with standard chow, while the other four groups were fed with adenine to make CRF. The rats in control group received intra-gastric normal saline (NS) of 20 mL/(kg · d) for 30 d, while those in low, medium and high Fushengong Decoction dose groups received Fushengong Decoction at the dose of 4 g/kg, 8 g/kg and 16 g/kg respectively, once a day for 30 d. After that, 24 h urinary protein in urine was measured, blood urea nitrogen (BUN) and serum creatinine (SCr) were detected. Histomorphology of glomerulus were studied by HE staining, and the expression of nephrin were detected by immunohistochemistry and RT-PCR.RESULTS:Compared to the control group, the levels of 24 h urinary protein, BUN and SCr increased significantly (P < 0.05) and the expression of nephrin protein and mRNA decreased significantly (P < 0.05) in CRF model group. The renal interstitium showed fibrotic lesions in model group. The levels of 24 h urinary protein, BUN and SCr decreased significantly after the treatment of Fushengong Decoction (P < 0.05), while the expression of nephrin protein and mRNA increased significantly (P < 0.05).CONCLUSION:Fushengong Decoction could reduce urinary protein and relieve renal damage in rats with CRF by improving the expression of nephrin and reducing the injury of podocytes.

目的 观察复肾功方治疗慢性肾功能衰竭的可能作用机制.方法 将55只SD大鼠随机分为正常组、模型组及复肾功方低、中、高剂量组,每组11只.正常组自由饮食,其余4组大鼠食用含0.5％腺嘌呤饲料制造慢性肾功能衰竭模型.造模后正常组和模型组给予生理盐水20 ml/kg灌胃;复肾功方低、中、高剂量组分别以4、8、16 g/kg灌胃复肾功方,每日1次,连续30天.检测各组大鼠血肌酐(SCr)和尿素氮(BUN)水平,HE染色检测肾组织形态学改变,免疫组化检测音猬因子(Shh)、神经胶质瘤相关癌基因同源蛋白1 (Gli1)的表达. 结果 与正常组比较,模型组大鼠SCr和BUN水平及肾组织Shh、Gli1水平明显升高(P＜0.05),肾间质出现明显纤维化病变.复肾功方各剂量组大鼠SCr和BUN水平及肾组织Shh、Gli1水平较模型组均明显降低,其中复肾功方高剂量组各指标水平最低,3组间两两比较差异有统计学意义(P＜0.05).结论 复肾功方能明显改善慢性肾功能衰竭大鼠肾功能,且随剂量增加改善效果更好,其机制可能与抑制Shh信号通路的激活有关.

Objective:To investigate the effect of Tongfeng Xiaoting Decoction on the inflammatory factors in ankle joint synovial tissue of acute gouty arthritis rats(AGA).Method:60 male SD rats were randomly divided into 6 groups:normal group, model group, colchicine group and low, medium and high Tongfeng Xiaoting Decoction groups (10 in each group).AGA model was established by induced monosodium urate (MSU) method except the normal group. Two days before AGA model was established, rats in normal and model groups received intra-gastric normal saline(NS) by 20ml/kg.d for qd, colchicine group received colchicine by 0.3mg/kg.d for qd, while those in low, medium and high Tongfeng Xiaoting Decoction groups received Tongfeng Xiaoting Decoction at the liquid of 2g /kg,4g /kg and 8g/kg respectively, once daily, until 7 days after model was formed, continuously for 9 days. After the experiment, serum uric acid was detected, the expression of IL-1β、IL-6、TNF-α、TGF-β1 were detected by ELISA and the expression of TGF-β1 and TGF-βR1 were detected by immunohistochemistry. Result:Serum uric acid and the content of IL-1β、IL-6、TNF-α、TGF-β1 in ankle joint synovial membrane were increased significantly in the model group compared to that of the normal group(P<0.05).Serum uric acid and the content of IL-1β、IL-6、TNF-α、TGF-β1 in ankle joint synovial membrane were decreased significantly in Tongfeng Xiaoting Decoction groups compared to that of the model group(P<0.05). The expression of TGF-β1and TGF-βR1 in ankle joint synovial membrane were increased significantly in Tongfeng Xiaoting Decoction groups compared to that of the others group(P<0.05).Conclusion: The mechanism of treating gouty arthritis by Tongfeng Xiaoting Decoction may be through up-regulating the expression of TGF-β1 ,TGF-rβ1 and inhibiting the expression of IL-1β、IL-6、TNF-αin synovial tissue.