Objective:To comparative investigate the HPV16 virus-specific immune characteristics of HPV vaccinees, cervical precancerous lesions patients and cervical cancer patients.Methods:Six female HPV vaccinees, five patients with cervical precancerous lesions or cervical cancer and 8 healthy female volunteers from Beijing were recruited as subjects. Peripheral anticoagulated blood specimens were collected from these subjects and the peripheral blood mononuclear cells (PBMCs) and plasma were isolated. After culturing in vitro with the stimulation of L1 protein peptide pool derived from HPV16 for 9-10 days, the percentage of CD4 + and CD8 + -T lymphocytes secreting cytokines IFN-γ, IL-2 and TNF-α in the PBMCs were detected by flow cytometry. The level of HPV16 IgG antibody in plasma was determined by ELISA. Results:The IgG levels of vaccinees were significantly higher than those of healthy donors as well as patients with cervical precancerous lesions or cervical cancer. Moreover, the specific CD4 + T cell immune level of vaccinees against HPV16 was significantly higher than that of healthy controls. Furthermore, there was a positive correlation between the level of specific T cell immunity and the level of antibody against HPV16 in all the donors (Pearson, r>0.4, p<0.05). Conclusions:The virus-specific immune responses, i. e. humoral responses as well as cellular immune responses are presenting diversely among different HPV-related subjects. HPV vaccinees possess robust humoral immunity and CD4 + T cell responses.

女性绝经前后,由于性激素波动或减少,多数女性会出现一系列以自主神经系统功能紊乱为主,伴有神经心理症状的症候群,这就是更年期综合征. 更年期多发生于45岁～55岁,90％的妇女可出现轻重不等的症状,有人在绝经过渡期症状已开始出现,持续到绝经后2年～3年,少数人可持续到绝经后5年～10年症状才有所减轻或消失. 每位女性都会经历更年期.虽然这是个生理过程,但“生理现象”并非都是友善的,当影响到人们正常生活,甚至给健康带来威胁时,这个特殊时期就需要专门对待了.若不妥善应对,绝经带来的相关症状、导致的各种疾病将会极大影响女性的生活质量.

目的 探讨低频电刺激治疗产后乳量不足的疗效.方法 选取顺产分娩的初产妇203例,随机分为治疗组102例和对照组101例.治疗组采用低频电刺激治疗,对照组仅予母乳喂养指导.比较两组产妇治疗后泌乳情况.结果 治疗组促进泌乳效果明显优于对照组,差异有统计学意义(P<0.05).结论 低频电刺激治疗能有效促进产后乳汁分泌,效果明显,值得临床推广应用.

目的:观察生物电刺激治疗产后慢性子宫复旧不全的临床疗效.方法将100例产后42天仍阴道出血的产妇随机分为治疗组52例和对照组48例.治疗组采用生物电刺激治疗,对照组未予治疗.结果治疗组子宫复旧总有效率为86.5％,高于对照组的50.0％,治疗组疗效优于对照组,差异有统计学意义(P<0.05).结论:生物电刺激治疗可有效促进产后子宫复旧,安全可靠,经济方便,且无明显不良反应,值得临床推广应用.

目的 观察生物电刺激治疗薄型子宫内膜的临床疗效.方法 选择40例薄型子宫内膜患者,随机分为实验组及对照组各20例,实验组采用生物电刺激治疗,对照组不予治疗,观察比较两组子宫内膜变化.结果 实验组疗效明显优于对照组,差异有统计学意义(P<0.05).结论:生物电刺激治疗薄型子宫内膜,疗效明显且副作用少,值得临床推广应用.

目的 探讨坤泰胶囊配合人工周期治疗卵巢储备功能下降所致月经稀发的疗效.方法 选择门诊符合卵巢储备功能下降所致月经稀发入选标准的患者,采用坤泰胶囊配合人工周期治疗,观察疗效.结果 治疗总有效率达100%.结论 坤泰胶囊配合人工周期治疗卵巢储备功能下降所致月经稀发疗效显著,不良反应少,安全性高.