目的对海藻酸钙和卡波姆凝胶经支气管镜介入兔肺支气管内的安全性进行评价,为含抗结核药物凝胶肺内应用提供依据。方法 45只日本长耳兔随机分成对照组、海藻酸钙组和卡波姆组,每组15只,实验观测4周。检测体温、体重、血常规、T细胞亚群及肺病理学改变。结果各组间体温、体重、血常规及外周血T细胞亚群的比较均无显著性差异(P>0.05)。海藻酸钙组和卡波姆组凝胶介入前与介入后CD3+、CD4+、CD8+百分比比较亦无显著性差异(P>0.05)。海藻酸钙组和卡波姆组的受试动物左肺底细支气管无凝胶成分残留,肺大体及光镜病理观察与对照组相比均未产生明显变化。光镜病理观察可见海藻酸钙组的右肺底有局灶性肺泡破裂,肺泡间隔无炎性细胞浸润。卡波姆凝胶组的左肺底偶见局灶性肺不张。结论海藻酸钙和卡波姆凝胶肺内应用均具有较好的安全性,可作为含抗结核药物凝胶的良好基质。

Objective To evaluate the effect of carbomer gel and e-gum bronchoscopy intervention in the rabbit lung and provide evidence for using antituberculosis medicament gel administered in lung.Methods Forty-five Japanese long-eared rabbits were randomly divided into control group,carbomer group and e-gum group.Animal temperature,avoirdupois,hemic routine,T lymphocyte subsets,and pathology were tested.Results There were no statistical difference in animal temperature,avoirdupois,hemic routine and T lymphocyte subsets of each group.There were no statistical difference in percent of CD3+,CD4+,CD8+between before and after injection in carbomer group and e-gum group.Lung tissue pathology of carbomer group was no abnormality.There was no carbomer gel in left lung.In e-gum group,a small amount of gel was found in left bronchia of the rabbit.One of rabbits got pneumonia and lymphocyte was found in Spacing of alveolus. Conclusions Carbomer gel and e-gum can be used in lung for security and be good medium to develop antituberculosis medicament gel.